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A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro

Samo za registrovane korisnike
2008
Autori
Bajić, Vladan
Đelić, Ninoslav
Potparević, Biljana
Živković, Lada
Milicević, Z.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p lt 0.05) only at the highest dose (15 mu ...M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.

Izvor:
Russian Journal of Physical Chemistry A, 2008, 44, 5, 546-552
Izdavač:
  • Maik Nauka/Interperiodica/Springer, New York
Projekti:
  • Evaluacija dejstva hormona i citostatika prmenom citogenetičkih analiza i Komet testa (RS-143018)

DOI: 10.1134/S1022795408050062

ISSN: 1022-7954

WoS: 000256092000006

Scopus: 2-s2.0-44349130457
[ Google Scholar ]
2
2
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1072
Kolekcije
  • Radovi istraživača / Researchers’ publications
Institucija
Pharmacy
TY  - JOUR
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Milicević, Z.
PY  - 2008
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1072
AB  - 8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p  lt  0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Russian Journal of Physical Chemistry A
T1  - A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro
VL  - 44
IS  - 5
SP  - 546
EP  - 552
DO  - 10.1134/S1022795408050062
ER  - 
@article{
author = "Bajić, Vladan and Đelić, Ninoslav and Potparević, Biljana and Živković, Lada and Milicević, Z.",
year = "2008",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/1072",
abstract = "8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p  lt  0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Russian Journal of Physical Chemistry A",
title = "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro",
volume = "44",
number = "5",
pages = "546-552",
doi = "10.1134/S1022795408050062"
}
Bajić V, Đelić N, Potparević B, Živković L, Milicević Z. A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. Russian Journal of Physical Chemistry A. 2008;44(5):546-552
Bajić, V., Đelić, N., Potparević, B., Živković, L.,& Milicević, Z. (2008). A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro.
Russian Journal of Physical Chemistry AMaik Nauka/Interperiodica/Springer, New York., 44(5), 546-552.
https://doi.org/10.1134/S1022795408050062
Bajić Vladan, Đelić Ninoslav, Potparević Biljana, Živković Lada, Milicević Z., "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro" 44, no. 5 (2008):546-552,
https://doi.org/10.1134/S1022795408050062 .

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