A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro
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2008
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8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p lt 0.05) only at the highest dose (15 mu ...M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.
Izvor:
Russian Journal of Physical Chemistry A, 2008, 44, 5, 546-552Izdavač:
- Maik Nauka/Interperiodica/Springer, New York
Finansiranje / projekti:
DOI: 10.1134/S1022795408050062
ISSN: 1022-7954
WoS: 000256092000006
Scopus: 2-s2.0-44349130457
Institucija/grupa
PharmacyTY - JOUR AU - Bajić, Vladan AU - Đelić, Ninoslav AU - Potparević, Biljana AU - Živković, Lada AU - Milicević, Z. PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1072 AB - 8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p lt 0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Russian Journal of Physical Chemistry A T1 - A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro VL - 44 IS - 5 SP - 546 EP - 552 DO - 10.1134/S1022795408050062 ER -
@article{ author = "Bajić, Vladan and Đelić, Ninoslav and Potparević, Biljana and Živković, Lada and Milicević, Z.", year = "2008", abstract = "8-chloro-cyclic adenosine 3',5'-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p lt 0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Russian Journal of Physical Chemistry A", title = "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro", volume = "44", number = "5", pages = "546-552", doi = "10.1134/S1022795408050062" }
Bajić, V., Đelić, N., Potparević, B., Živković, L.,& Milicević, Z.. (2008). A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Physical Chemistry A Maik Nauka/Interperiodica/Springer, New York., 44(5), 546-552. https://doi.org/10.1134/S1022795408050062
Bajić V, Đelić N, Potparević B, Živković L, Milicević Z. A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Physical Chemistry A. 2008;44(5):546-552. doi:10.1134/S1022795408050062 .
Bajić, Vladan, Đelić, Ninoslav, Potparević, Biljana, Živković, Lada, Milicević, Z., "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro" in Russian Journal of Physical Chemistry A, 44, no. 5 (2008):546-552, https://doi.org/10.1134/S1022795408050062 . .