Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals
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Miseljić, BranislavaPopović, Gordana
Agbaba, Danica
Marković, Slavko
Simonovska, Breda
Vovk, Irena
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A gradient reversed-phase column high-performance liquid chromatographic method was developed for the detection and quantification of norfloxacin and its major impurities in norfloxacin-containing pharmaceuticals. Chromatographic separations were performed under the following experimental conditions: column, Zorbax SB RP-18 (5 mu m, 250 x 4.6 mm); injection volume, 20 mu L; mobile phase, 0.05 M NaH2PO4 (pH 2.5)-acetonitrile (87 + 13) for 16 min and (58 + 42) for 9 min (stepwise gradient); and flow rate, 1.3 mL/min. All analyses were performed at 25 degrees C, and the eluate was monitored at 275 nm using a diode array detector. Linearity (correlation coefficient = 0.999), recovery (99.3-101.8%), relative standard deviation (0.2-0.7%), and quantitation limit (0.12-0.47 mu g/mL) were evaluated and found to be satisfactory. The method is simple, rapid, and convenient for purity control of norfloxacin in both raw materials and dosage forms.
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Journal of AOAC International, 2008, 91, 2, 332-338Publisher:
- AOAC Int, Gaithersburg
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PharmacyTY - JOUR AU - Miseljić, Branislava AU - Popović, Gordana AU - Agbaba, Danica AU - Marković, Slavko AU - Simonovska, Breda AU - Vovk, Irena PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1077 AB - A gradient reversed-phase column high-performance liquid chromatographic method was developed for the detection and quantification of norfloxacin and its major impurities in norfloxacin-containing pharmaceuticals. Chromatographic separations were performed under the following experimental conditions: column, Zorbax SB RP-18 (5 mu m, 250 x 4.6 mm); injection volume, 20 mu L; mobile phase, 0.05 M NaH2PO4 (pH 2.5)-acetonitrile (87 + 13) for 16 min and (58 + 42) for 9 min (stepwise gradient); and flow rate, 1.3 mL/min. All analyses were performed at 25 degrees C, and the eluate was monitored at 275 nm using a diode array detector. Linearity (correlation coefficient = 0.999), recovery (99.3-101.8%), relative standard deviation (0.2-0.7%), and quantitation limit (0.12-0.47 mu g/mL) were evaluated and found to be satisfactory. The method is simple, rapid, and convenient for purity control of norfloxacin in both raw materials and dosage forms. PB - AOAC Int, Gaithersburg T2 - Journal of AOAC International T1 - Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals VL - 91 IS - 2 SP - 332 EP - 338 UR - https://hdl.handle.net/21.15107/rcub_farfar_1077 ER -
@article{ author = "Miseljić, Branislava and Popović, Gordana and Agbaba, Danica and Marković, Slavko and Simonovska, Breda and Vovk, Irena", year = "2008", abstract = "A gradient reversed-phase column high-performance liquid chromatographic method was developed for the detection and quantification of norfloxacin and its major impurities in norfloxacin-containing pharmaceuticals. Chromatographic separations were performed under the following experimental conditions: column, Zorbax SB RP-18 (5 mu m, 250 x 4.6 mm); injection volume, 20 mu L; mobile phase, 0.05 M NaH2PO4 (pH 2.5)-acetonitrile (87 + 13) for 16 min and (58 + 42) for 9 min (stepwise gradient); and flow rate, 1.3 mL/min. All analyses were performed at 25 degrees C, and the eluate was monitored at 275 nm using a diode array detector. Linearity (correlation coefficient = 0.999), recovery (99.3-101.8%), relative standard deviation (0.2-0.7%), and quantitation limit (0.12-0.47 mu g/mL) were evaluated and found to be satisfactory. The method is simple, rapid, and convenient for purity control of norfloxacin in both raw materials and dosage forms.", publisher = "AOAC Int, Gaithersburg", journal = "Journal of AOAC International", title = "Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals", volume = "91", number = "2", pages = "332-338", url = "https://hdl.handle.net/21.15107/rcub_farfar_1077" }
Miseljić, B., Popović, G., Agbaba, D., Marković, S., Simonovska, B.,& Vovk, I.. (2008). Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals. in Journal of AOAC International AOAC Int, Gaithersburg., 91(2), 332-338. https://hdl.handle.net/21.15107/rcub_farfar_1077
Miseljić B, Popović G, Agbaba D, Marković S, Simonovska B, Vovk I. Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals. in Journal of AOAC International. 2008;91(2):332-338. https://hdl.handle.net/21.15107/rcub_farfar_1077 .
Miseljić, Branislava, Popović, Gordana, Agbaba, Danica, Marković, Slavko, Simonovska, Breda, Vovk, Irena, "Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals" in Journal of AOAC International, 91, no. 2 (2008):332-338, https://hdl.handle.net/21.15107/rcub_farfar_1077 .