Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography
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2008
Authors
Injac, RadeMlinarić, Ales
Đorđević-Milić, Vukosava

Karljiković-Rajić, Katarina

Štrukelj, Borut
Article (Published version)

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A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, po...lymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.
Keywords:
animal feed additives / Zn bacitracin / oxytetracycline / polymyxin B / sulfacetamide / micellar electrokinetic capillary chromatography (MEKC)Source:
Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment, 2008, 25, 4, 424-431Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
DOI: 10.1080/02652030701584058
ISSN: 1944-0049
PubMed: 18348041
WoS: 000254071100005
Scopus: 2-s2.0-41049110221
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PharmacyTY - JOUR AU - Injac, Rade AU - Mlinarić, Ales AU - Đorđević-Milić, Vukosava AU - Karljiković-Rajić, Katarina AU - Štrukelj, Borut PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1103 AB - A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed. PB - Taylor & Francis Ltd, Abingdon T2 - Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment T1 - Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography VL - 25 IS - 4 SP - 424 EP - 431 DO - 10.1080/02652030701584058 ER -
@article{ author = "Injac, Rade and Mlinarić, Ales and Đorđević-Milić, Vukosava and Karljiković-Rajić, Katarina and Štrukelj, Borut", year = "2008", abstract = "A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment", title = "Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography", volume = "25", number = "4", pages = "424-431", doi = "10.1080/02652030701584058" }
Injac, R., Mlinarić, A., Đorđević-Milić, V., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment Taylor & Francis Ltd, Abingdon., 25(4), 424-431. https://doi.org/10.1080/02652030701584058
Injac R, Mlinarić A, Đorđević-Milić V, Karljiković-Rajić K, Štrukelj B. Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment. 2008;25(4):424-431. doi:10.1080/02652030701584058 .
Injac, Rade, Mlinarić, Ales, Đorđević-Milić, Vukosava, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography" in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment, 25, no. 4 (2008):424-431, https://doi.org/10.1080/02652030701584058 . .