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Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography

Authorized Users Only
2008
Authors
Injac, Rade
Mlinarić, Ales
Đorđević-Milić, Vukosava
Karljiković-Rajić, Katarina
Štrukelj, Borut
Article (Published version)
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Abstract
A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, po...lymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.

Keywords:
animal feed additives / Zn bacitracin / oxytetracycline / polymyxin B / sulfacetamide / micellar electrokinetic capillary chromatography (MEKC)
Source:
Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment, 2008, 25, 4, 424-431
Publisher:
  • Taylor & Francis Ltd, Abingdon
Funding / projects:
  • Supstance za farmaceutsku upotrebu: modeliranje, sinteza, fizičko-hemijske i biološke osobine, stepen čistoće i ispitivanje doziranih oblika (RS-142072)

DOI: 10.1080/02652030701584058

ISSN: 1944-0049

PubMed: 18348041

WoS: 000254071100005

Scopus: 2-s2.0-41049110221
[ Google Scholar ]
27
21
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/1103
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Injac, Rade
AU  - Mlinarić, Ales
AU  - Đorđević-Milić, Vukosava
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1103
AB  - A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment
T1  - Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography
VL  - 25
IS  - 4
SP  - 424
EP  - 431
DO  - 10.1080/02652030701584058
ER  - 
@article{
author = "Injac, Rade and Mlinarić, Ales and Đorđević-Milić, Vukosava and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment",
title = "Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography",
volume = "25",
number = "4",
pages = "424-431",
doi = "10.1080/02652030701584058"
}
Injac, R., Mlinarić, A., Đorđević-Milić, V., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment
Taylor & Francis Ltd, Abingdon., 25(4), 424-431.
https://doi.org/10.1080/02652030701584058
Injac R, Mlinarić A, Đorđević-Milić V, Karljiković-Rajić K, Štrukelj B. Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment. 2008;25(4):424-431.
doi:10.1080/02652030701584058 .
Injac, Rade, Mlinarić, Ales, Đorđević-Milić, Vukosava, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography" in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment, 25, no. 4 (2008):424-431,
https://doi.org/10.1080/02652030701584058 . .

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