Beneficial effects of dimethyl fumarate on experimental autoimmune myocarditis
Samo za registrovane korisnike
2008
Autori
Milenković, Marina
Arsenović-Ranin, Nevena

Božić, Dragana

Bufan, Biljana

Jančić, Ivan

Stojić-Vukanić, Zorica

Članak u časopisu (Objavljena verzija)

Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background. Fumaric acid esters (FAE) have been proven to be effective for the systemic treatment of psoriasis and multiple sclerosis, Thl cell-mediated chronic inflammatory diseases, but their effect on autoimmune rnyocarditis has not yet been addressed. We investigated the effect of dimethyl fumarate (DMF) on myosin-induced experimental autoimmune myocarditis (EAM). Methods. Dark Agouti (DA) rats immunized with porcine cardiac myosin were orally treated with 5 and 15 mg/kg body weight (bw) DMF either from days 0-10 (early treatment groups) or from days 10-21 (late treatment groups) after induction of EAM. All rats were sacrificed on day 21 after immunization and hearts were evaluated macroscopically and microscopically. Levels of TNF-alpha and IL-10 in serum and lymph node cells culture supernatants were detected by ELISA. Results. Both early and late treatment with 15 mg/kg body weight (bw) DMF markedly C, reduced the severity of myocarditis by comparing the incidence, heart weight ...bw ratio, macroscopic and microscopic scores, and number of OX-6+ cells in the myocardium. Further, levels of tumor necrosis factor-alpha (TNF-alpha) in serum and culture supernatants of lymph node cells stimulated with ConA or myosin were significantly lower in DNIF-treated EAM animals compared with vehicle-treated EAM rats. There was no significant difference in serum levels of interleukin-10 between DMF- and vehicle-treated EAM rats. Conclusions. These results show for the first time that DMF ameliorates experimental autoimmune rnyocarditis and may be acted, at least in part, by interfering with the production of TNF-alpha.
Ključne reči:
rats / experimental autoimmune myocarditis / dimethyl fumarate / tumor necrosis factor-alpha / interleukin-10Izvor:
Archives of Medical Research, 2008, 39, 7, 639-646Izdavač:
- Elsevier Science Inc, New York
DOI: 10.1016/j.arcmed.2008.07.003
ISSN: 0188-4409
PubMed: 18760191
WoS: 000259418800002
Scopus: 2-s2.0-50049108903
Institucija/grupa
PharmacyTY - JOUR AU - Milenković, Marina AU - Arsenović-Ranin, Nevena AU - Božić, Dragana AU - Bufan, Biljana AU - Jančić, Ivan AU - Stojić-Vukanić, Zorica PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1107 AB - Background. Fumaric acid esters (FAE) have been proven to be effective for the systemic treatment of psoriasis and multiple sclerosis, Thl cell-mediated chronic inflammatory diseases, but their effect on autoimmune rnyocarditis has not yet been addressed. We investigated the effect of dimethyl fumarate (DMF) on myosin-induced experimental autoimmune myocarditis (EAM). Methods. Dark Agouti (DA) rats immunized with porcine cardiac myosin were orally treated with 5 and 15 mg/kg body weight (bw) DMF either from days 0-10 (early treatment groups) or from days 10-21 (late treatment groups) after induction of EAM. All rats were sacrificed on day 21 after immunization and hearts were evaluated macroscopically and microscopically. Levels of TNF-alpha and IL-10 in serum and lymph node cells culture supernatants were detected by ELISA. Results. Both early and late treatment with 15 mg/kg body weight (bw) DMF markedly C, reduced the severity of myocarditis by comparing the incidence, heart weight bw ratio, macroscopic and microscopic scores, and number of OX-6+ cells in the myocardium. Further, levels of tumor necrosis factor-alpha (TNF-alpha) in serum and culture supernatants of lymph node cells stimulated with ConA or myosin were significantly lower in DNIF-treated EAM animals compared with vehicle-treated EAM rats. There was no significant difference in serum levels of interleukin-10 between DMF- and vehicle-treated EAM rats. Conclusions. These results show for the first time that DMF ameliorates experimental autoimmune rnyocarditis and may be acted, at least in part, by interfering with the production of TNF-alpha. PB - Elsevier Science Inc, New York T2 - Archives of Medical Research T1 - Beneficial effects of dimethyl fumarate on experimental autoimmune myocarditis VL - 39 IS - 7 SP - 639 EP - 646 DO - 10.1016/j.arcmed.2008.07.003 ER -
@article{ author = "Milenković, Marina and Arsenović-Ranin, Nevena and Božić, Dragana and Bufan, Biljana and Jančić, Ivan and Stojić-Vukanić, Zorica", year = "2008", abstract = "Background. Fumaric acid esters (FAE) have been proven to be effective for the systemic treatment of psoriasis and multiple sclerosis, Thl cell-mediated chronic inflammatory diseases, but their effect on autoimmune rnyocarditis has not yet been addressed. We investigated the effect of dimethyl fumarate (DMF) on myosin-induced experimental autoimmune myocarditis (EAM). Methods. Dark Agouti (DA) rats immunized with porcine cardiac myosin were orally treated with 5 and 15 mg/kg body weight (bw) DMF either from days 0-10 (early treatment groups) or from days 10-21 (late treatment groups) after induction of EAM. All rats were sacrificed on day 21 after immunization and hearts were evaluated macroscopically and microscopically. Levels of TNF-alpha and IL-10 in serum and lymph node cells culture supernatants were detected by ELISA. Results. Both early and late treatment with 15 mg/kg body weight (bw) DMF markedly C, reduced the severity of myocarditis by comparing the incidence, heart weight bw ratio, macroscopic and microscopic scores, and number of OX-6+ cells in the myocardium. Further, levels of tumor necrosis factor-alpha (TNF-alpha) in serum and culture supernatants of lymph node cells stimulated with ConA or myosin were significantly lower in DNIF-treated EAM animals compared with vehicle-treated EAM rats. There was no significant difference in serum levels of interleukin-10 between DMF- and vehicle-treated EAM rats. Conclusions. These results show for the first time that DMF ameliorates experimental autoimmune rnyocarditis and may be acted, at least in part, by interfering with the production of TNF-alpha.", publisher = "Elsevier Science Inc, New York", journal = "Archives of Medical Research", title = "Beneficial effects of dimethyl fumarate on experimental autoimmune myocarditis", volume = "39", number = "7", pages = "639-646", doi = "10.1016/j.arcmed.2008.07.003" }
Milenković, M., Arsenović-Ranin, N., Božić, D., Bufan, B., Jančić, I.,& Stojić-Vukanić, Z.. (2008). Beneficial effects of dimethyl fumarate on experimental autoimmune myocarditis. in Archives of Medical Research Elsevier Science Inc, New York., 39(7), 639-646. https://doi.org/10.1016/j.arcmed.2008.07.003
Milenković M, Arsenović-Ranin N, Božić D, Bufan B, Jančić I, Stojić-Vukanić Z. Beneficial effects of dimethyl fumarate on experimental autoimmune myocarditis. in Archives of Medical Research. 2008;39(7):639-646. doi:10.1016/j.arcmed.2008.07.003 .
Milenković, Marina, Arsenović-Ranin, Nevena, Božić, Dragana, Bufan, Biljana, Jančić, Ivan, Stojić-Vukanić, Zorica, "Beneficial effects of dimethyl fumarate on experimental autoimmune myocarditis" in Archives of Medical Research, 39, no. 7 (2008):639-646, https://doi.org/10.1016/j.arcmed.2008.07.003 . .