In vitro antioxidant activity of ragweed (Ambrosia artemisiifolia L., Asteraceae) herb
Abstract
The antioxidant activity in 70% aqueous acetone extracts of the herb of Ambrosia artemisiifolia L., Asteraceae was evaluated with regards to total polyphenol and flavonoid contents. Antioxidant activity has been assessed by two commonly used in vitro tests, based on determination of ferric-reducing antioxidant power (FRAP assay) and DPPH free radical scavenging ability (DPPH assay), against butylated hydroxytoluene (BHT), ascorbic acid and quercetin, which were used as positive control substances. The results of both antioxidant tests showed that the plant material expressed a considerable activity (DPPH IC50 = 27.6 mu g/ml; FRAP value = 2.37 mmol Fe2+/g), attributed to both flavonoid aglyca and resembling glycosides, as verified by dot-blot TLC analysis.
Keywords:
Ambrosia artemisiifolia / antioxidant activity / FRAP assay / DPPH assay / flavonoids / polyphenolsSource:
Industrial Crops and Products, 2008, 28, 3, 356-360Publisher:
- Elsevier Science BV, Amsterdam
Funding / projects:
- Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija (RS-143012)
DOI: 10.1016/j.indcrop.2008.04.001
ISSN: 0926-6690
WoS: 000259437100015
Scopus: 2-s2.0-49749132004
Collections
Institution/Community
PharmacyTY - JOUR AU - Maksimović, Zoran PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1108 AB - The antioxidant activity in 70% aqueous acetone extracts of the herb of Ambrosia artemisiifolia L., Asteraceae was evaluated with regards to total polyphenol and flavonoid contents. Antioxidant activity has been assessed by two commonly used in vitro tests, based on determination of ferric-reducing antioxidant power (FRAP assay) and DPPH free radical scavenging ability (DPPH assay), against butylated hydroxytoluene (BHT), ascorbic acid and quercetin, which were used as positive control substances. The results of both antioxidant tests showed that the plant material expressed a considerable activity (DPPH IC50 = 27.6 mu g/ml; FRAP value = 2.37 mmol Fe2+/g), attributed to both flavonoid aglyca and resembling glycosides, as verified by dot-blot TLC analysis. PB - Elsevier Science BV, Amsterdam T2 - Industrial Crops and Products T1 - In vitro antioxidant activity of ragweed (Ambrosia artemisiifolia L., Asteraceae) herb VL - 28 IS - 3 SP - 356 EP - 360 DO - 10.1016/j.indcrop.2008.04.001 ER -
@article{ author = "Maksimović, Zoran", year = "2008", abstract = "The antioxidant activity in 70% aqueous acetone extracts of the herb of Ambrosia artemisiifolia L., Asteraceae was evaluated with regards to total polyphenol and flavonoid contents. Antioxidant activity has been assessed by two commonly used in vitro tests, based on determination of ferric-reducing antioxidant power (FRAP assay) and DPPH free radical scavenging ability (DPPH assay), against butylated hydroxytoluene (BHT), ascorbic acid and quercetin, which were used as positive control substances. The results of both antioxidant tests showed that the plant material expressed a considerable activity (DPPH IC50 = 27.6 mu g/ml; FRAP value = 2.37 mmol Fe2+/g), attributed to both flavonoid aglyca and resembling glycosides, as verified by dot-blot TLC analysis.", publisher = "Elsevier Science BV, Amsterdam", journal = "Industrial Crops and Products", title = "In vitro antioxidant activity of ragweed (Ambrosia artemisiifolia L., Asteraceae) herb", volume = "28", number = "3", pages = "356-360", doi = "10.1016/j.indcrop.2008.04.001" }
Maksimović, Z.. (2008). In vitro antioxidant activity of ragweed (Ambrosia artemisiifolia L., Asteraceae) herb. in Industrial Crops and Products Elsevier Science BV, Amsterdam., 28(3), 356-360. https://doi.org/10.1016/j.indcrop.2008.04.001
Maksimović Z. In vitro antioxidant activity of ragweed (Ambrosia artemisiifolia L., Asteraceae) herb. in Industrial Crops and Products. 2008;28(3):356-360. doi:10.1016/j.indcrop.2008.04.001 .
Maksimović, Zoran, "In vitro antioxidant activity of ragweed (Ambrosia artemisiifolia L., Asteraceae) herb" in Industrial Crops and Products, 28, no. 3 (2008):356-360, https://doi.org/10.1016/j.indcrop.2008.04.001 . .