Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography
Abstract
A micellar electrokinetic capillary chromatography was performed at 25 degrees C and 30 kV (under pressure of 15 mbar), with 30 mM berate buffer (pH 9.0), 60 mM sodium dodecysulfate, and 10% (v/v) ethanol as background electrolyte for the determination of sulfamethoxazole and trimethoprim. UV detection was at 205 nm. Recoveries were optimal and acceptable after extraction with ethanol / deionized water (1:1, v/v) for both investigated compounds from laboratory mixtures of standards. The method was shown to be specific, accurate (recoveries were 99.9 +/- 0.4% for sulfamethoxazole and 99.8 +/- 0.3% for trimethoprim), linear over the tested ranges (correlation coefficients >= 0.9990) and precise (RSD below 0.6%). The method was applied to determine sulfamethoxazole and trimethoprim in tablets, powder for cutaneous use and solution for infusion.
Keywords:
sulfamethoxazole / trimethoprim / LLE / micellar electrokinetic capillary chromatography (MEKC) / pharmaceuticalsSource:
Journal of Food and Drug Analysis, 2008, 16, 1, 18-25Publisher:
- Food & Drug Adminstration, Taipei
Collections
Institution
PharmacyTY - JOUR AU - Injac, Rade AU - Kac, Javor AU - Karljiković-Rajić, Katarina AU - Štrukelj, Borut PY - 2008 UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1110 AB - A micellar electrokinetic capillary chromatography was performed at 25 degrees C and 30 kV (under pressure of 15 mbar), with 30 mM berate buffer (pH 9.0), 60 mM sodium dodecysulfate, and 10% (v/v) ethanol as background electrolyte for the determination of sulfamethoxazole and trimethoprim. UV detection was at 205 nm. Recoveries were optimal and acceptable after extraction with ethanol / deionized water (1:1, v/v) for both investigated compounds from laboratory mixtures of standards. The method was shown to be specific, accurate (recoveries were 99.9 +/- 0.4% for sulfamethoxazole and 99.8 +/- 0.3% for trimethoprim), linear over the tested ranges (correlation coefficients >= 0.9990) and precise (RSD below 0.6%). The method was applied to determine sulfamethoxazole and trimethoprim in tablets, powder for cutaneous use and solution for infusion. PB - Food & Drug Adminstration, Taipei T2 - Journal of Food and Drug Analysis T1 - Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography VL - 16 IS - 1 SP - 18 EP - 25 ER -
@article{ author = "Injac, Rade and Kac, Javor and Karljiković-Rajić, Katarina and Štrukelj, Borut", year = "2008", url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/1110", abstract = "A micellar electrokinetic capillary chromatography was performed at 25 degrees C and 30 kV (under pressure of 15 mbar), with 30 mM berate buffer (pH 9.0), 60 mM sodium dodecysulfate, and 10% (v/v) ethanol as background electrolyte for the determination of sulfamethoxazole and trimethoprim. UV detection was at 205 nm. Recoveries were optimal and acceptable after extraction with ethanol / deionized water (1:1, v/v) for both investigated compounds from laboratory mixtures of standards. The method was shown to be specific, accurate (recoveries were 99.9 +/- 0.4% for sulfamethoxazole and 99.8 +/- 0.3% for trimethoprim), linear over the tested ranges (correlation coefficients >= 0.9990) and precise (RSD below 0.6%). The method was applied to determine sulfamethoxazole and trimethoprim in tablets, powder for cutaneous use and solution for infusion.", publisher = "Food & Drug Adminstration, Taipei", journal = "Journal of Food and Drug Analysis", title = "Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography", volume = "16", number = "1", pages = "18-25" }
Injac R, Kac J, Karljiković-Rajić K, Štrukelj B. Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography. Journal of Food and Drug Analysis. 2008;16(1):18-25
Injac, R., Kac, J., Karljiković-Rajić, K.,& Štrukelj, B. (2008). Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography. Journal of Food and Drug AnalysisFood & Drug Adminstration, Taipei., 16(1), 18-25.
Injac Rade, Kac Javor, Karljiković-Rajić Katarina, Štrukelj Borut, "Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography" 16, no. 1 (2008):18-25