The chromatography methods: High performance liquid chromatography and thin layer chromatography

Abstract

Chromatographic methods represent the most frequently used methods in both qualitative and quantitative studies of various samples. High performance and thin layer chromatography have important place among these methods. They are widely applied in many scientific and technological researches. The choice of specific chromatographic method depends on the type of research, and sometimes two or more methods are combined. The main features, advantages and disadvantages of the two most widely used chromatographic methods has been discussed in this short presentation.

Hromatografske metode se danas često primenjuju kako u kvalitativnim tako i u kvantitativnim ispitivanjima različitih uzoraka. Među njima značajno mesto zauzimaju visoko-efikasna tečna i tankoslojna hromatografija koje se često takmiče u mogućnostima svoje primene. Izbor hromatografske metode u raznim oblastima nauke i tehnologije (medicina, farmaceutska industrija, industrija hrane, kozmetologija, ekologija) najviše zavisi od problema koji treba rešiti, pri čemu često jedna metoda nije dovoljna već je potrebno kombinovati različite hromatografske tehnike.