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dc.creatorVujić, Zorica
dc.creatorCrevar, Milkica
dc.creatorObradović, Vladimir
dc.creatorKuntić, Vesna
dc.creatorUskoković-Marković, Snežana
dc.date.accessioned2019-09-02T11:15:45Z
dc.date.available2019-09-02T11:15:45Z
dc.date.issued2009
dc.identifier.issn0009-5893
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1160
dc.description.abstractA rapid and simple liquid chromatographic method with UV detection has been developed for the determination of hydrochlorothiazide (HCTZ), cilazapril (CL) and its active metabolite cilazaprilat (CLT) in urine. Sample preparation for urine consisted of solid-phase extraction using styrene-divinylbenzene (SDB-2) cartridges. The chromatographic system was a Zorbax Eclipse XDB-C-18 column with a mixture of methanol and 10 mM phosphate buffer, pH 2.3 with gradient (20 to 60% of methanol) as mobile phase at a flow rate of 1.0 mL min(-1). The detection was performed at the wavelength of 206 nm. Enalapril maleat was used as an internal standard. The detector response was linear in the range of 2.4-30.0, 1.6-15.0 and 1.8-20.0 mu g mL(-1) for HCTZ, CL and CLT, respectively. LOQ was determined to be 2.4, 1.6 and 1.8 mu g mL(-1) for HCTZ, CL and CLT, respectively. Both intra- and inter-day precision were within acceptable limits. The method has been applied to urine samples obtained from three hypertensive patients after intake of HCTZ and CL therapeutic dose.en
dc.publisherSpringer Heidelberg, Heidelberg
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142072/RS//
dc.rightsrestrictedAccess
dc.sourceChromatographia
dc.subjectColumn liquid chromatographyen
dc.subjectSolid phase extractionen
dc.subjectHydrochlorothiazide in urineen
dc.subjectCilazapril and cilazaprilaten
dc.titleSimultaneous Determination of Hydrochlorothiazide, Cilazapril and Its Active Metabolite Cilazaprilat in Urine by Gradient RP-LCen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractОбрадовић, Владимир; Кунтић, Весна; Цревар, Милкица; Ускоковић-Марковић, Снежана; Вујић, Зорица;
dc.citation.volume70
dc.citation.issue7-8
dc.citation.spage1221
dc.citation.epage1225
dc.citation.other70(7-8): 1221-1225
dc.citation.rankM23
dc.identifier.wos000271069400029
dc.identifier.doi10.1365/s10337-009-1286-6
dc.identifier.scopus2-s2.0-78149490514
dc.identifier.rcubconv_2239
dc.type.versionpublishedVersion


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