Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations
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2009
Authors
Ravanić, N.Filipić, Slavica

Nikolić, Katarina

Popović, Gordana

Vovk, Irena
Simonovska, Breda
Agbaba, Danica

Article (Published version)

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A simple and reliable TLC method for analysis of -lipoic acid ( LA) with post-chromatographic derivatisation with palladium(II) chloride immersion reagent has been developed and evaluated. Separation of LA was performed on 20 cm x 10 cm RPTLC plates with 2-propanol-methanol-acetone-water-acetic acid 6: 4: 2: 8: 0.2 (v/v) as mobile phase. Yellow complexes formed in situ were scanned at 375 nm. The migration distance of LA was 43.0 mm. The relationship between peak area and amount of LA applied was evaluated by use of linear (1.0-3.0 mu g per band) and second-degree polynomial ( 0.5-5.0 mu g per band) regression functions. The correlation coefficient (r = 0.999), the limit of quantification (0.39 mu g per band), recovery (98.5-105.2%), and precision (1.8-2.9%) obtained by use of the procedure were satisfactory. The method was used for analysis of LA in several drug formulations and selected dietary supplement preparations. The LA content was 99.5-101.0% in the drug formulations, 98.8-99....5% in three of five dietary supplements tested, and 48.0-185.0% in two other dietary supplements.
Keywords:
alpha-Lipoic acid / thin-layer chromatography / densitometry / dietary supplementsSource:
Acta Chromatographica, 2009, 21, 3, 433-441Publisher:
- Akademiai Kiado Zrt, Budapest
Funding / projects:
DOI: 10.1556/AChrom.21.2009.3.7
ISSN: 1233-2356
WoS: 000270187600007
Scopus: 2-s2.0-70349614946
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Institution/Community
PharmacyTY - JOUR AU - Ravanić, N. AU - Filipić, Slavica AU - Nikolić, Katarina AU - Popović, Gordana AU - Vovk, Irena AU - Simonovska, Breda AU - Agbaba, Danica PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1170 AB - A simple and reliable TLC method for analysis of -lipoic acid ( LA) with post-chromatographic derivatisation with palladium(II) chloride immersion reagent has been developed and evaluated. Separation of LA was performed on 20 cm x 10 cm RPTLC plates with 2-propanol-methanol-acetone-water-acetic acid 6: 4: 2: 8: 0.2 (v/v) as mobile phase. Yellow complexes formed in situ were scanned at 375 nm. The migration distance of LA was 43.0 mm. The relationship between peak area and amount of LA applied was evaluated by use of linear (1.0-3.0 mu g per band) and second-degree polynomial ( 0.5-5.0 mu g per band) regression functions. The correlation coefficient (r = 0.999), the limit of quantification (0.39 mu g per band), recovery (98.5-105.2%), and precision (1.8-2.9%) obtained by use of the procedure were satisfactory. The method was used for analysis of LA in several drug formulations and selected dietary supplement preparations. The LA content was 99.5-101.0% in the drug formulations, 98.8-99.5% in three of five dietary supplements tested, and 48.0-185.0% in two other dietary supplements. PB - Akademiai Kiado Zrt, Budapest T2 - Acta Chromatographica T1 - Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations VL - 21 IS - 3 SP - 433 EP - 441 DO - 10.1556/AChrom.21.2009.3.7 ER -
@article{ author = "Ravanić, N. and Filipić, Slavica and Nikolić, Katarina and Popović, Gordana and Vovk, Irena and Simonovska, Breda and Agbaba, Danica", year = "2009", abstract = "A simple and reliable TLC method for analysis of -lipoic acid ( LA) with post-chromatographic derivatisation with palladium(II) chloride immersion reagent has been developed and evaluated. Separation of LA was performed on 20 cm x 10 cm RPTLC plates with 2-propanol-methanol-acetone-water-acetic acid 6: 4: 2: 8: 0.2 (v/v) as mobile phase. Yellow complexes formed in situ were scanned at 375 nm. The migration distance of LA was 43.0 mm. The relationship between peak area and amount of LA applied was evaluated by use of linear (1.0-3.0 mu g per band) and second-degree polynomial ( 0.5-5.0 mu g per band) regression functions. The correlation coefficient (r = 0.999), the limit of quantification (0.39 mu g per band), recovery (98.5-105.2%), and precision (1.8-2.9%) obtained by use of the procedure were satisfactory. The method was used for analysis of LA in several drug formulations and selected dietary supplement preparations. The LA content was 99.5-101.0% in the drug formulations, 98.8-99.5% in three of five dietary supplements tested, and 48.0-185.0% in two other dietary supplements.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Acta Chromatographica", title = "Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations", volume = "21", number = "3", pages = "433-441", doi = "10.1556/AChrom.21.2009.3.7" }
Ravanić, N., Filipić, S., Nikolić, K., Popović, G., Vovk, I., Simonovska, B.,& Agbaba, D.. (2009). Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations. in Acta Chromatographica Akademiai Kiado Zrt, Budapest., 21(3), 433-441. https://doi.org/10.1556/AChrom.21.2009.3.7
Ravanić N, Filipić S, Nikolić K, Popović G, Vovk I, Simonovska B, Agbaba D. Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations. in Acta Chromatographica. 2009;21(3):433-441. doi:10.1556/AChrom.21.2009.3.7 .
Ravanić, N., Filipić, Slavica, Nikolić, Katarina, Popović, Gordana, Vovk, Irena, Simonovska, Breda, Agbaba, Danica, "Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations" in Acta Chromatographica, 21, no. 3 (2009):433-441, https://doi.org/10.1556/AChrom.21.2009.3.7 . .