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Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations

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Authors
Ravanić, N.
Filipić, Slavica
Nikolić, Katarina
Popović, Gordana
Vovk, Irena
Simonovska, Breda
Agbaba, Danica
Article (Published version)
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Abstract
A simple and reliable TLC method for analysis of -lipoic acid ( LA) with post-chromatographic derivatisation with palladium(II) chloride immersion reagent has been developed and evaluated. Separation of LA was performed on 20 cm x 10 cm RPTLC plates with 2-propanol-methanol-acetone-water-acetic acid 6: 4: 2: 8: 0.2 (v/v) as mobile phase. Yellow complexes formed in situ were scanned at 375 nm. The migration distance of LA was 43.0 mm. The relationship between peak area and amount of LA applied was evaluated by use of linear (1.0-3.0 mu g per band) and second-degree polynomial ( 0.5-5.0 mu g per band) regression functions. The correlation coefficient (r = 0.999), the limit of quantification (0.39 mu g per band), recovery (98.5-105.2%), and precision (1.8-2.9%) obtained by use of the procedure were satisfactory. The method was used for analysis of LA in several drug formulations and selected dietary supplement preparations. The LA content was 99.5-101.0% in the drug formulations, 98.8-99....5% in three of five dietary supplements tested, and 48.0-185.0% in two other dietary supplements.

Keywords:
alpha-Lipoic acid / thin-layer chromatography / densitometry / dietary supplements
Source:
Acta Chromatographica, 2009, 21, 3, 433-441
Publisher:
  • Akademiai Kiado Zrt, Budapest
Projects:
  • Sinteza, kvantitativni odnosi između strukture/osobina i aktivnosti, fizičko-hemijska karakterizacija i analiza farmakološki aktivnih supstanci (RS-142071)

DOI: 10.1556/AChrom.21.2009.3.7

ISSN: 1233-2356

WoS: 000270187600007

Scopus: 2-s2.0-70349614946
[ Google Scholar ]
2
2
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1170
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy
TY  - JOUR
AU  - Ravanić, N.
AU  - Filipić, Slavica
AU  - Nikolić, Katarina
AU  - Popović, Gordana
AU  - Vovk, Irena
AU  - Simonovska, Breda
AU  - Agbaba, Danica
PY  - 2009
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1170
AB  - A simple and reliable TLC method for analysis of -lipoic acid ( LA) with post-chromatographic derivatisation with palladium(II) chloride immersion reagent has been developed and evaluated. Separation of LA was performed on 20 cm x 10 cm RPTLC plates with 2-propanol-methanol-acetone-water-acetic acid 6: 4: 2: 8: 0.2 (v/v) as mobile phase. Yellow complexes formed in situ were scanned at 375 nm. The migration distance of LA was 43.0 mm. The relationship between peak area and amount of LA applied was evaluated by use of linear (1.0-3.0 mu g per band) and second-degree polynomial ( 0.5-5.0 mu g per band) regression functions. The correlation coefficient (r = 0.999), the limit of quantification (0.39 mu g per band), recovery (98.5-105.2%), and precision (1.8-2.9%) obtained by use of the procedure were satisfactory. The method was used for analysis of LA in several drug formulations and selected dietary supplement preparations. The LA content was 99.5-101.0% in the drug formulations, 98.8-99.5% in three of five dietary supplements tested, and 48.0-185.0% in two other dietary supplements.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Chromatographica
T1  - Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations
VL  - 21
IS  - 3
SP  - 433
EP  - 441
DO  - 10.1556/AChrom.21.2009.3.7
ER  - 
@article{
author = "Ravanić, N. and Filipić, Slavica and Nikolić, Katarina and Popović, Gordana and Vovk, Irena and Simonovska, Breda and Agbaba, Danica",
year = "2009",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/1170",
abstract = "A simple and reliable TLC method for analysis of -lipoic acid ( LA) with post-chromatographic derivatisation with palladium(II) chloride immersion reagent has been developed and evaluated. Separation of LA was performed on 20 cm x 10 cm RPTLC plates with 2-propanol-methanol-acetone-water-acetic acid 6: 4: 2: 8: 0.2 (v/v) as mobile phase. Yellow complexes formed in situ were scanned at 375 nm. The migration distance of LA was 43.0 mm. The relationship between peak area and amount of LA applied was evaluated by use of linear (1.0-3.0 mu g per band) and second-degree polynomial ( 0.5-5.0 mu g per band) regression functions. The correlation coefficient (r = 0.999), the limit of quantification (0.39 mu g per band), recovery (98.5-105.2%), and precision (1.8-2.9%) obtained by use of the procedure were satisfactory. The method was used for analysis of LA in several drug formulations and selected dietary supplement preparations. The LA content was 99.5-101.0% in the drug formulations, 98.8-99.5% in three of five dietary supplements tested, and 48.0-185.0% in two other dietary supplements.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Chromatographica",
title = "Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations",
volume = "21",
number = "3",
pages = "433-441",
doi = "10.1556/AChrom.21.2009.3.7"
}
Ravanić N, Filipić S, Nikolić K, Popović G, Vovk I, Simonovska B, Agbaba D. Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations. Acta Chromatographica. 2009;21(3):433-441
Ravanić, N., Filipić, S., Nikolić, K., Popović, G., Vovk, I., Simonovska, B.,& Agbaba, D. (2009). Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations.
Acta ChromatographicaAkademiai Kiado Zrt, Budapest., 21(3), 433-441.
https://doi.org/10.1556/AChrom.21.2009.3.7
Ravanić N., Filipić Slavica, Nikolić Katarina, Popović Gordana, Vovk Irena, Simonovska Breda, Agbaba Danica, "Analysis of alpha-Lipoic Acid in Drug Formulations and Dietary Supplement Preparations" 21, no. 3 (2009):433-441,
https://doi.org/10.1556/AChrom.21.2009.3.7 .

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