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Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats

Authorized Users Only
2009
Authors
Stojić-Vukanić, Zorica
Rauski, Aleksandra
Kosec, Duško
Radojević, Katarina
Pilipović, Ivan
Leposavić, Gordana
Article (Published version)
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Abstract
A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP... TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009

Keywords:
adrenalectomy / thymic hypercellularity / thymocyte apoptosis / thymocyte development
Source:
Experimental Biology and Medicine, 2009, 234, 9, 1067-1074
Publisher:
  • Royal Soc Medicine Press Ltd, London
Projects:
  • Neuroendokrina modulacija imunskog odgovora: uloga simpato-adrenomedularnog sistema (RS-145049)

DOI: 10.3181/0902-RM-63

ISSN: 1535-3702

PubMed: 19546352

WoS: 000269966600008

Scopus: 2-s2.0-70349128618
[ Google Scholar ]
11
11
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1197
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy
TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Rauski, Aleksandra
AU  - Kosec, Duško
AU  - Radojević, Katarina
AU  - Pilipović, Ivan
AU  - Leposavić, Gordana
PY  - 2009
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1197
AB  - A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009
PB  - Royal Soc Medicine Press Ltd, London
T2  - Experimental Biology and Medicine
T1  - Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats
VL  - 234
IS  - 9
SP  - 1067
EP  - 1074
DO  - 10.3181/0902-RM-63
ER  - 
@article{
author = "Stojić-Vukanić, Zorica and Rauski, Aleksandra and Kosec, Duško and Radojević, Katarina and Pilipović, Ivan and Leposavić, Gordana",
year = "2009",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/1197",
abstract = "A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009",
publisher = "Royal Soc Medicine Press Ltd, London",
journal = "Experimental Biology and Medicine",
title = "Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats",
volume = "234",
number = "9",
pages = "1067-1074",
doi = "10.3181/0902-RM-63"
}
Stojić-Vukanić Z, Rauski A, Kosec D, Radojević K, Pilipović I, Leposavić G. Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats. Experimental Biology and Medicine. 2009;234(9):1067-1074
Stojić-Vukanić, Z., Rauski, A., Kosec, D., Radojević, K., Pilipović, I.,& Leposavić, G. (2009). Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats.
Experimental Biology and MedicineRoyal Soc Medicine Press Ltd, London., 234(9), 1067-1074.
https://doi.org/10.3181/0902-RM-63
Stojić-Vukanić Zorica, Rauski Aleksandra, Kosec Duško, Radojević Katarina, Pilipović Ivan, Leposavić Gordana, "Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats" 234, no. 9 (2009):1067-1074,
https://doi.org/10.3181/0902-RM-63 .

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