Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats
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2009
Authors
Stojić-Vukanić, ZoricaRauski, Aleksandra
Kosec, Duško
Radojević, Katarina
Pilipović, Ivan
Leposavić, Gordana
Article (Published version)
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A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP... TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009
Keywords:
adrenalectomy / thymic hypercellularity / thymocyte apoptosis / thymocyte developmentSource:
Experimental Biology and Medicine, 2009, 234, 9, 1067-1074Publisher:
- Royal Soc Medicine Press Ltd, London
Funding / projects:
DOI: 10.3181/0902-RM-63
ISSN: 1535-3702
PubMed: 19546352
WoS: 000269966600008
Scopus: 2-s2.0-70349128618
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PharmacyTY - JOUR AU - Stojić-Vukanić, Zorica AU - Rauski, Aleksandra AU - Kosec, Duško AU - Radojević, Katarina AU - Pilipović, Ivan AU - Leposavić, Gordana PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1197 AB - A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009 PB - Royal Soc Medicine Press Ltd, London T2 - Experimental Biology and Medicine T1 - Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats VL - 234 IS - 9 SP - 1067 EP - 1074 DO - 10.3181/0902-RM-63 ER -
@article{ author = "Stojić-Vukanić, Zorica and Rauski, Aleksandra and Kosec, Duško and Radojević, Katarina and Pilipović, Ivan and Leposavić, Gordana", year = "2009", abstract = "A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009", publisher = "Royal Soc Medicine Press Ltd, London", journal = "Experimental Biology and Medicine", title = "Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats", volume = "234", number = "9", pages = "1067-1074", doi = "10.3181/0902-RM-63" }
Stojić-Vukanić, Z., Rauski, A., Kosec, D., Radojević, K., Pilipović, I.,& Leposavić, G.. (2009). Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats. in Experimental Biology and Medicine Royal Soc Medicine Press Ltd, London., 234(9), 1067-1074. https://doi.org/10.3181/0902-RM-63
Stojić-Vukanić Z, Rauski A, Kosec D, Radojević K, Pilipović I, Leposavić G. Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats. in Experimental Biology and Medicine. 2009;234(9):1067-1074. doi:10.3181/0902-RM-63 .
Stojić-Vukanić, Zorica, Rauski, Aleksandra, Kosec, Duško, Radojević, Katarina, Pilipović, Ivan, Leposavić, Gordana, "Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats" in Experimental Biology and Medicine, 234, no. 9 (2009):1067-1074, https://doi.org/10.3181/0902-RM-63 . .