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dc.creatorStojić-Vukanić, Zorica
dc.creatorRauski, Aleksandra
dc.creatorKosec, Duško
dc.creatorRadojević, Katarina
dc.creatorPilipović, Ivan
dc.creatorLeposavić, Gordana
dc.date.accessioned2019-09-02T11:16:46Z
dc.date.available2019-09-02T11:16:46Z
dc.date.issued2009
dc.identifier.issn1535-3702
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1197
dc.description.abstractA number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009en
dc.publisherRoyal Soc Medicine Press Ltd, London
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/145049/RS//
dc.rightsrestrictedAccess
dc.sourceExperimental Biology and Medicine
dc.subjectadrenalectomyen
dc.subjectthymic hypercellularityen
dc.subjectthymocyte apoptosisen
dc.subjectthymocyte developmenten
dc.titleDysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Ratsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРауски, Aлександра; Стојић-Вуканић, Зорица; Радојевић, Катарина; Косец, Душко; Пилиповић, Иван; Лепосавић, Гордана;
dc.citation.volume234
dc.citation.issue9
dc.citation.spage1067
dc.citation.epage1074
dc.citation.other234(9): 1067-1074
dc.citation.rankM22
dc.identifier.wos000269966600008
dc.identifier.doi10.3181/0902-RM-63
dc.identifier.pmid19546352
dc.identifier.scopus2-s2.0-70349128618
dc.identifier.rcubconv_2216
dc.type.versionpublishedVersion


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