dc.creator | Stojić-Vukanić, Zorica | |
dc.creator | Rauski, Aleksandra | |
dc.creator | Kosec, Duško | |
dc.creator | Radojević, Katarina | |
dc.creator | Pilipović, Ivan | |
dc.creator | Leposavić, Gordana | |
dc.date.accessioned | 2019-09-02T11:16:46Z | |
dc.date.available | 2019-09-02T11:16:46Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1535-3702 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/1197 | |
dc.description.abstract | A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009 | en |
dc.publisher | Royal Soc Medicine Press Ltd, London | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/145049/RS// | |
dc.rights | restrictedAccess | |
dc.source | Experimental Biology and Medicine | |
dc.subject | adrenalectomy | en |
dc.subject | thymic hypercellularity | en |
dc.subject | thymocyte apoptosis | en |
dc.subject | thymocyte development | en |
dc.title | Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Рауски, Aлександра; Стојић-Вуканић, Зорица; Радојевић, Катарина; Косец, Душко; Пилиповић, Иван; Лепосавић, Гордана; | |
dc.citation.volume | 234 | |
dc.citation.issue | 9 | |
dc.citation.spage | 1067 | |
dc.citation.epage | 1074 | |
dc.citation.other | 234(9): 1067-1074 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000269966600008 | |
dc.identifier.doi | 10.3181/0902-RM-63 | |
dc.identifier.pmid | 19546352 | |
dc.identifier.scopus | 2-s2.0-70349128618 | |
dc.type.version | publishedVersion | |