Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms
Apstrakt
A sensitive spectrophotometric method for the determination of metoprolol tartrate in tablets and ampoules is presented. Using spectrophotometric measurements, it was found that metoprolol tartrate and benzyl orange form a chloroform soluble ion-pair complex with an absorption maximum at 401 nm. The composition of the ion-pair complex was determined by applying Job's method to equimolar solutions of metoprolol tartrate: benzyl orange (1:2); molar absorptivity 7.39 x 103 mol-1 cm-1. Extraction of the ion-pair complex in chloroform was accomplished easily at a Britton-Robinson's buffered optimum pH = 5.2, μ = 0.1 mol/dm3. The relative stability constant, calculated according to the method of Sommer and Job's non-equimolar solutions, was log K = 9.72 (avg. value). Beer's law was obeyed up to 3.42 μg/ml of metoprolol tartrate (the detection limit was also 3.42 μg/ml). The precision of the method was checked at three different concentrations. The RSD (n = 7) varied from 0.51 to 2.03%. Repro...ducibility was examined by analysing Lopresor® tablets and ampoules. Recoveries varied from 99-101%. The reported method, applied to the assay of metoprolol tartrate in tablets and ampoules, gives precise and reproducible results.
Izvor:
Farmaco, 1995, 50, 4, 281-284Institucija/grupa
PharmacyTY - JOUR AU - Vujić, Zorica AU - Radulović, Dušanka AU - Živanović, Ljiljana PY - 1995 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/119 AB - A sensitive spectrophotometric method for the determination of metoprolol tartrate in tablets and ampoules is presented. Using spectrophotometric measurements, it was found that metoprolol tartrate and benzyl orange form a chloroform soluble ion-pair complex with an absorption maximum at 401 nm. The composition of the ion-pair complex was determined by applying Job's method to equimolar solutions of metoprolol tartrate: benzyl orange (1:2); molar absorptivity 7.39 x 103 mol-1 cm-1. Extraction of the ion-pair complex in chloroform was accomplished easily at a Britton-Robinson's buffered optimum pH = 5.2, μ = 0.1 mol/dm3. The relative stability constant, calculated according to the method of Sommer and Job's non-equimolar solutions, was log K = 9.72 (avg. value). Beer's law was obeyed up to 3.42 μg/ml of metoprolol tartrate (the detection limit was also 3.42 μg/ml). The precision of the method was checked at three different concentrations. The RSD (n = 7) varied from 0.51 to 2.03%. Reproducibility was examined by analysing Lopresor® tablets and ampoules. Recoveries varied from 99-101%. The reported method, applied to the assay of metoprolol tartrate in tablets and ampoules, gives precise and reproducible results. T2 - Farmaco T1 - Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms VL - 50 IS - 4 SP - 281 EP - 284 UR - https://hdl.handle.net/21.15107/rcub_farfar_119 ER -
@article{ author = "Vujić, Zorica and Radulović, Dušanka and Živanović, Ljiljana", year = "1995", abstract = "A sensitive spectrophotometric method for the determination of metoprolol tartrate in tablets and ampoules is presented. Using spectrophotometric measurements, it was found that metoprolol tartrate and benzyl orange form a chloroform soluble ion-pair complex with an absorption maximum at 401 nm. The composition of the ion-pair complex was determined by applying Job's method to equimolar solutions of metoprolol tartrate: benzyl orange (1:2); molar absorptivity 7.39 x 103 mol-1 cm-1. Extraction of the ion-pair complex in chloroform was accomplished easily at a Britton-Robinson's buffered optimum pH = 5.2, μ = 0.1 mol/dm3. The relative stability constant, calculated according to the method of Sommer and Job's non-equimolar solutions, was log K = 9.72 (avg. value). Beer's law was obeyed up to 3.42 μg/ml of metoprolol tartrate (the detection limit was also 3.42 μg/ml). The precision of the method was checked at three different concentrations. The RSD (n = 7) varied from 0.51 to 2.03%. Reproducibility was examined by analysing Lopresor® tablets and ampoules. Recoveries varied from 99-101%. The reported method, applied to the assay of metoprolol tartrate in tablets and ampoules, gives precise and reproducible results.", journal = "Farmaco", title = "Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms", volume = "50", number = "4", pages = "281-284", url = "https://hdl.handle.net/21.15107/rcub_farfar_119" }
Vujić, Z., Radulović, D.,& Živanović, L.. (1995). Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms. in Farmaco, 50(4), 281-284. https://hdl.handle.net/21.15107/rcub_farfar_119
Vujić Z, Radulović D, Živanović L. Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms. in Farmaco. 1995;50(4):281-284. https://hdl.handle.net/21.15107/rcub_farfar_119 .
Vujić, Zorica, Radulović, Dušanka, Živanović, Ljiljana, "Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms" in Farmaco, 50, no. 4 (1995):281-284, https://hdl.handle.net/21.15107/rcub_farfar_119 .