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Spectrofluorimetric and HPLC Determination of Morin in Human Serum

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2009
1203.pdf (78.27Kb)
Authors
Pavun, Leposava
Đikanović, Daniela
Đurđević, Predrag
Jelikić-Stankov, Milena
Malešev, Dušan
Ćirić, Andrija
Article (Published version)
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Abstract
Morin is a flavonol antioxidant. In ethanol-water mixtures (70 wt% of ethanol) it reacts with Al(3+) to give Al(Morin)(2) in the pH range 3-6. The conditional stability constant of this complex at 298 K was found to be log beta(2) = 16.96 +/- 0.02 at pH 4.40. The complex shows strong fluorescence emission at 500 nm upon excitation at 410 nm. The fluorescence intensity is pH dependent with maximum emission at pH 4.40. Since the complexation reaction enhances the fluorescence of morin, this property was used for the determination of morin in human serum. A linear dependence of the intensity of fluorescence of the complex on the concentration of morin was obtained in morin concentration range from 1.5-30.5 ng mL(-1), relative standard error of measurements was 1.4%. The LOD was 0.02 ng mL(-1) while LOQ was 1.0 ng mL(-1). Serum concentration of morin was also determined using HPLC as a reference method. A C-18 Hypersil Gold AQ column was used with acetonitrile-0.1% v/v phosphoric acid (30:...70% v/v) as the mobile phase at 1.0 mL min(-1) flow rate and UV detection at 250 nm. Acceptable relative standard errors (less than 5%) between determinations obtained by the two methods indicate that the fluorescence method is reliable.

Keywords:
Morin / aluminium / determination / spectrofluorimetry / serum
Source:
Acta Chimica Slovenica, 2009, 56, 4, 967-972
Publisher:
  • Slovensko Kemijsko Drustvo, Ljubljana
Funding / projects:
  • Bioaktivni kompleksi p i d jona metala sa ligandima farmakoterapijskog značaja (RS-142013)

ISSN: 1318-0207

WoS: 000273279400026

Scopus: 2-s2.0-74849114036
[ Google Scholar ]
20
17
Handle
https://hdl.handle.net/21.15107/rcub_farfar_1205
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/1205
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Pavun, Leposava
AU  - Đikanović, Daniela
AU  - Đurđević, Predrag
AU  - Jelikić-Stankov, Milena
AU  - Malešev, Dušan
AU  - Ćirić, Andrija
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1205
AB  - Morin is a flavonol antioxidant. In ethanol-water mixtures (70 wt% of ethanol) it reacts with Al(3+) to give Al(Morin)(2) in the pH range 3-6. The conditional stability constant of this complex at 298 K was found to be log beta(2) = 16.96 +/- 0.02 at pH 4.40. The complex shows strong fluorescence emission at 500 nm upon excitation at 410 nm. The fluorescence intensity is pH dependent with maximum emission at pH 4.40. Since the complexation reaction enhances the fluorescence of morin, this property was used for the determination of morin in human serum. A linear dependence of the intensity of fluorescence of the complex on the concentration of morin was obtained in morin concentration range from 1.5-30.5 ng mL(-1), relative standard error of measurements was 1.4%. The LOD was 0.02 ng mL(-1) while LOQ was 1.0 ng mL(-1). Serum concentration of morin was also determined using HPLC as a reference method. A C-18 Hypersil Gold AQ column was used with acetonitrile-0.1% v/v phosphoric acid (30:70% v/v) as the mobile phase at 1.0 mL min(-1) flow rate and UV detection at 250 nm. Acceptable relative standard errors (less than 5%) between determinations obtained by the two methods indicate that the fluorescence method is reliable.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Spectrofluorimetric and HPLC Determination of Morin in Human Serum
VL  - 56
IS  - 4
SP  - 967
EP  - 972
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1205
ER  - 
@article{
author = "Pavun, Leposava and Đikanović, Daniela and Đurđević, Predrag and Jelikić-Stankov, Milena and Malešev, Dušan and Ćirić, Andrija",
year = "2009",
abstract = "Morin is a flavonol antioxidant. In ethanol-water mixtures (70 wt% of ethanol) it reacts with Al(3+) to give Al(Morin)(2) in the pH range 3-6. The conditional stability constant of this complex at 298 K was found to be log beta(2) = 16.96 +/- 0.02 at pH 4.40. The complex shows strong fluorescence emission at 500 nm upon excitation at 410 nm. The fluorescence intensity is pH dependent with maximum emission at pH 4.40. Since the complexation reaction enhances the fluorescence of morin, this property was used for the determination of morin in human serum. A linear dependence of the intensity of fluorescence of the complex on the concentration of morin was obtained in morin concentration range from 1.5-30.5 ng mL(-1), relative standard error of measurements was 1.4%. The LOD was 0.02 ng mL(-1) while LOQ was 1.0 ng mL(-1). Serum concentration of morin was also determined using HPLC as a reference method. A C-18 Hypersil Gold AQ column was used with acetonitrile-0.1% v/v phosphoric acid (30:70% v/v) as the mobile phase at 1.0 mL min(-1) flow rate and UV detection at 250 nm. Acceptable relative standard errors (less than 5%) between determinations obtained by the two methods indicate that the fluorescence method is reliable.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Spectrofluorimetric and HPLC Determination of Morin in Human Serum",
volume = "56",
number = "4",
pages = "967-972",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1205"
}
Pavun, L., Đikanović, D., Đurđević, P., Jelikić-Stankov, M., Malešev, D.,& Ćirić, A.. (2009). Spectrofluorimetric and HPLC Determination of Morin in Human Serum. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 56(4), 967-972.
https://hdl.handle.net/21.15107/rcub_farfar_1205
Pavun L, Đikanović D, Đurđević P, Jelikić-Stankov M, Malešev D, Ćirić A. Spectrofluorimetric and HPLC Determination of Morin in Human Serum. in Acta Chimica Slovenica. 2009;56(4):967-972.
https://hdl.handle.net/21.15107/rcub_farfar_1205 .
Pavun, Leposava, Đikanović, Daniela, Đurđević, Predrag, Jelikić-Stankov, Milena, Malešev, Dušan, Ćirić, Andrija, "Spectrofluorimetric and HPLC Determination of Morin in Human Serum" in Acta Chimica Slovenica, 56, no. 4 (2009):967-972,
https://hdl.handle.net/21.15107/rcub_farfar_1205 .

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