Spectrofluorimetric and HPLC Determination of Morin in Human Serum

2009
Authors
Pavun, Leposava
Đikanović, Daniela
Đurđević, Predrag

Jelikić-Stankov, Milena
Malešev, Dušan
Ćirić, Andrija

Article (Published version)
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Morin is a flavonol antioxidant. In ethanol-water mixtures (70 wt% of ethanol) it reacts with Al(3+) to give Al(Morin)(2) in the pH range 3-6. The conditional stability constant of this complex at 298 K was found to be log beta(2) = 16.96 +/- 0.02 at pH 4.40. The complex shows strong fluorescence emission at 500 nm upon excitation at 410 nm. The fluorescence intensity is pH dependent with maximum emission at pH 4.40. Since the complexation reaction enhances the fluorescence of morin, this property was used for the determination of morin in human serum. A linear dependence of the intensity of fluorescence of the complex on the concentration of morin was obtained in morin concentration range from 1.5-30.5 ng mL(-1), relative standard error of measurements was 1.4%. The LOD was 0.02 ng mL(-1) while LOQ was 1.0 ng mL(-1). Serum concentration of morin was also determined using HPLC as a reference method. A C-18 Hypersil Gold AQ column was used with acetonitrile-0.1% v/v phosphoric acid (30:...70% v/v) as the mobile phase at 1.0 mL min(-1) flow rate and UV detection at 250 nm. Acceptable relative standard errors (less than 5%) between determinations obtained by the two methods indicate that the fluorescence method is reliable.
Keywords:
Morin / aluminium / determination / spectrofluorimetry / serumSource:
Acta Chimica Slovenica, 2009, 56, 4, 967-972Publisher:
- Slovensko Kemijsko Drustvo, Ljubljana
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PharmacyTY - JOUR AU - Pavun, Leposava AU - Đikanović, Daniela AU - Đurđević, Predrag AU - Jelikić-Stankov, Milena AU - Malešev, Dušan AU - Ćirić, Andrija PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1205 AB - Morin is a flavonol antioxidant. In ethanol-water mixtures (70 wt% of ethanol) it reacts with Al(3+) to give Al(Morin)(2) in the pH range 3-6. The conditional stability constant of this complex at 298 K was found to be log beta(2) = 16.96 +/- 0.02 at pH 4.40. The complex shows strong fluorescence emission at 500 nm upon excitation at 410 nm. The fluorescence intensity is pH dependent with maximum emission at pH 4.40. Since the complexation reaction enhances the fluorescence of morin, this property was used for the determination of morin in human serum. A linear dependence of the intensity of fluorescence of the complex on the concentration of morin was obtained in morin concentration range from 1.5-30.5 ng mL(-1), relative standard error of measurements was 1.4%. The LOD was 0.02 ng mL(-1) while LOQ was 1.0 ng mL(-1). Serum concentration of morin was also determined using HPLC as a reference method. A C-18 Hypersil Gold AQ column was used with acetonitrile-0.1% v/v phosphoric acid (30:70% v/v) as the mobile phase at 1.0 mL min(-1) flow rate and UV detection at 250 nm. Acceptable relative standard errors (less than 5%) between determinations obtained by the two methods indicate that the fluorescence method is reliable. PB - Slovensko Kemijsko Drustvo, Ljubljana T2 - Acta Chimica Slovenica T1 - Spectrofluorimetric and HPLC Determination of Morin in Human Serum VL - 56 IS - 4 SP - 967 EP - 972 UR - https://hdl.handle.net/21.15107/rcub_farfar_1205 ER -
@article{ author = "Pavun, Leposava and Đikanović, Daniela and Đurđević, Predrag and Jelikić-Stankov, Milena and Malešev, Dušan and Ćirić, Andrija", year = "2009", abstract = "Morin is a flavonol antioxidant. In ethanol-water mixtures (70 wt% of ethanol) it reacts with Al(3+) to give Al(Morin)(2) in the pH range 3-6. The conditional stability constant of this complex at 298 K was found to be log beta(2) = 16.96 +/- 0.02 at pH 4.40. The complex shows strong fluorescence emission at 500 nm upon excitation at 410 nm. The fluorescence intensity is pH dependent with maximum emission at pH 4.40. Since the complexation reaction enhances the fluorescence of morin, this property was used for the determination of morin in human serum. A linear dependence of the intensity of fluorescence of the complex on the concentration of morin was obtained in morin concentration range from 1.5-30.5 ng mL(-1), relative standard error of measurements was 1.4%. The LOD was 0.02 ng mL(-1) while LOQ was 1.0 ng mL(-1). Serum concentration of morin was also determined using HPLC as a reference method. A C-18 Hypersil Gold AQ column was used with acetonitrile-0.1% v/v phosphoric acid (30:70% v/v) as the mobile phase at 1.0 mL min(-1) flow rate and UV detection at 250 nm. Acceptable relative standard errors (less than 5%) between determinations obtained by the two methods indicate that the fluorescence method is reliable.", publisher = "Slovensko Kemijsko Drustvo, Ljubljana", journal = "Acta Chimica Slovenica", title = "Spectrofluorimetric and HPLC Determination of Morin in Human Serum", volume = "56", number = "4", pages = "967-972", url = "https://hdl.handle.net/21.15107/rcub_farfar_1205" }
Pavun, L., Đikanović, D., Đurđević, P., Jelikić-Stankov, M., Malešev, D.,& Ćirić, A.. (2009). Spectrofluorimetric and HPLC Determination of Morin in Human Serum. in Acta Chimica Slovenica Slovensko Kemijsko Drustvo, Ljubljana., 56(4), 967-972. https://hdl.handle.net/21.15107/rcub_farfar_1205
Pavun L, Đikanović D, Đurđević P, Jelikić-Stankov M, Malešev D, Ćirić A. Spectrofluorimetric and HPLC Determination of Morin in Human Serum. in Acta Chimica Slovenica. 2009;56(4):967-972. https://hdl.handle.net/21.15107/rcub_farfar_1205 .
Pavun, Leposava, Đikanović, Daniela, Đurđević, Predrag, Jelikić-Stankov, Milena, Malešev, Dušan, Ćirić, Andrija, "Spectrofluorimetric and HPLC Determination of Morin in Human Serum" in Acta Chimica Slovenica, 56, no. 4 (2009):967-972, https://hdl.handle.net/21.15107/rcub_farfar_1205 .