HPLC method for determination of oximes HI-6 and trimedoxime in plasma
HPLC metoda za odredivanje oksima hi-6 i trimedoksima u plazmi
Апстракт
A high-performance liquid chromatographic (HPLC) assay was developed to determine HI-6 and trimedoxime concentrations in small volumes of plasma. The HPLC system comprised a high-pressure pump (model 2150), a multiwavelenght detector (model 2140) set at 284 nm and 295 nm for detection of trimedoxime and HI-6, respectively, and a sample injector (Rheodyne, model 7125) with a 20 μL sample loop. A Superpac starter kit 5 μm ODS 2; 4 x 250 mm (Pharmacia LKB) stainless steel column was used. To plasma sample, 10 μL of 20% trichlorocetic acid, was added. From the supernatant, 20 μL was injected directly into HPLC. The solvent was 25% acetonitrile in 0.08 mol/L acetic acid (pH 3) containing 0.01 mol/L heptane-1-sulfonic acid (sodium salt) and 0.0025 mol/L tetramethylammonium chloride. The flow rate was 0.8 mL/min HI-6, and trimedoxime eluted at about 6.0 min and 8.0 min, respectively. The limit of sensitivity was 1.4 μmol/L of trimedoxime and 2.5 μmol/L of HI-6. Recovery rates were 99.02% in d...etermination of HI-6 and 100.6% in determination of trimedoxime. Coefficients of variation for HI-6 and trimedoxime ranged from 1.4% to 8.2% and 0.9% to 3.4%, respectively. Our results indicate that the method is sensitive, accurate and precise.
Кључне речи:
Estimation / HI-6 / HPLC method / TrimedoxineИзвор:
Jugoslovenska medicinska biohemija, 1995, 14, 3-4, 125-129Институција/група
PharmacyTY - JOUR AU - Milić, B AU - Nedeljković, M AU - Đukić, Mirjana PY - 1995 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/124 AB - A high-performance liquid chromatographic (HPLC) assay was developed to determine HI-6 and trimedoxime concentrations in small volumes of plasma. The HPLC system comprised a high-pressure pump (model 2150), a multiwavelenght detector (model 2140) set at 284 nm and 295 nm for detection of trimedoxime and HI-6, respectively, and a sample injector (Rheodyne, model 7125) with a 20 μL sample loop. A Superpac starter kit 5 μm ODS 2; 4 x 250 mm (Pharmacia LKB) stainless steel column was used. To plasma sample, 10 μL of 20% trichlorocetic acid, was added. From the supernatant, 20 μL was injected directly into HPLC. The solvent was 25% acetonitrile in 0.08 mol/L acetic acid (pH 3) containing 0.01 mol/L heptane-1-sulfonic acid (sodium salt) and 0.0025 mol/L tetramethylammonium chloride. The flow rate was 0.8 mL/min HI-6, and trimedoxime eluted at about 6.0 min and 8.0 min, respectively. The limit of sensitivity was 1.4 μmol/L of trimedoxime and 2.5 μmol/L of HI-6. Recovery rates were 99.02% in determination of HI-6 and 100.6% in determination of trimedoxime. Coefficients of variation for HI-6 and trimedoxime ranged from 1.4% to 8.2% and 0.9% to 3.4%, respectively. Our results indicate that the method is sensitive, accurate and precise. T2 - Jugoslovenska medicinska biohemija T1 - HPLC method for determination of oximes HI-6 and trimedoxime in plasma T1 - HPLC metoda za odredivanje oksima hi-6 i trimedoksima u plazmi VL - 14 IS - 3-4 SP - 125 EP - 129 UR - https://hdl.handle.net/21.15107/rcub_farfar_124 ER -
@article{ author = "Milić, B and Nedeljković, M and Đukić, Mirjana", year = "1995", abstract = "A high-performance liquid chromatographic (HPLC) assay was developed to determine HI-6 and trimedoxime concentrations in small volumes of plasma. The HPLC system comprised a high-pressure pump (model 2150), a multiwavelenght detector (model 2140) set at 284 nm and 295 nm for detection of trimedoxime and HI-6, respectively, and a sample injector (Rheodyne, model 7125) with a 20 μL sample loop. A Superpac starter kit 5 μm ODS 2; 4 x 250 mm (Pharmacia LKB) stainless steel column was used. To plasma sample, 10 μL of 20% trichlorocetic acid, was added. From the supernatant, 20 μL was injected directly into HPLC. The solvent was 25% acetonitrile in 0.08 mol/L acetic acid (pH 3) containing 0.01 mol/L heptane-1-sulfonic acid (sodium salt) and 0.0025 mol/L tetramethylammonium chloride. The flow rate was 0.8 mL/min HI-6, and trimedoxime eluted at about 6.0 min and 8.0 min, respectively. The limit of sensitivity was 1.4 μmol/L of trimedoxime and 2.5 μmol/L of HI-6. Recovery rates were 99.02% in determination of HI-6 and 100.6% in determination of trimedoxime. Coefficients of variation for HI-6 and trimedoxime ranged from 1.4% to 8.2% and 0.9% to 3.4%, respectively. Our results indicate that the method is sensitive, accurate and precise.", journal = "Jugoslovenska medicinska biohemija", title = "HPLC method for determination of oximes HI-6 and trimedoxime in plasma, HPLC metoda za odredivanje oksima hi-6 i trimedoksima u plazmi", volume = "14", number = "3-4", pages = "125-129", url = "https://hdl.handle.net/21.15107/rcub_farfar_124" }
Milić, B., Nedeljković, M.,& Đukić, M.. (1995). HPLC method for determination of oximes HI-6 and trimedoxime in plasma. in Jugoslovenska medicinska biohemija, 14(3-4), 125-129. https://hdl.handle.net/21.15107/rcub_farfar_124
Milić B, Nedeljković M, Đukić M. HPLC method for determination of oximes HI-6 and trimedoxime in plasma. in Jugoslovenska medicinska biohemija. 1995;14(3-4):125-129. https://hdl.handle.net/21.15107/rcub_farfar_124 .
Milić, B, Nedeljković, M, Đukić, Mirjana, "HPLC method for determination of oximes HI-6 and trimedoxime in plasma" in Jugoslovenska medicinska biohemija, 14, no. 3-4 (1995):125-129, https://hdl.handle.net/21.15107/rcub_farfar_124 .