HPLC method for determination of oximes HI-6 and trimedoxime in plasma
HPLC metoda za odredivanje oksima hi-6 i trimedoksima u plazmi
dc.creator | Milić, B | |
dc.creator | Nedeljković, M | |
dc.creator | Đukić, Mirjana | |
dc.date.accessioned | 2019-09-02T10:49:04Z | |
dc.date.available | 2019-09-02T10:49:04Z | |
dc.date.issued | 1995 | |
dc.identifier.issn | 0352-1311 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/124 | |
dc.description.abstract | A high-performance liquid chromatographic (HPLC) assay was developed to determine HI-6 and trimedoxime concentrations in small volumes of plasma. The HPLC system comprised a high-pressure pump (model 2150), a multiwavelenght detector (model 2140) set at 284 nm and 295 nm for detection of trimedoxime and HI-6, respectively, and a sample injector (Rheodyne, model 7125) with a 20 μL sample loop. A Superpac starter kit 5 μm ODS 2; 4 x 250 mm (Pharmacia LKB) stainless steel column was used. To plasma sample, 10 μL of 20% trichlorocetic acid, was added. From the supernatant, 20 μL was injected directly into HPLC. The solvent was 25% acetonitrile in 0.08 mol/L acetic acid (pH 3) containing 0.01 mol/L heptane-1-sulfonic acid (sodium salt) and 0.0025 mol/L tetramethylammonium chloride. The flow rate was 0.8 mL/min HI-6, and trimedoxime eluted at about 6.0 min and 8.0 min, respectively. The limit of sensitivity was 1.4 μmol/L of trimedoxime and 2.5 μmol/L of HI-6. Recovery rates were 99.02% in determination of HI-6 and 100.6% in determination of trimedoxime. Coefficients of variation for HI-6 and trimedoxime ranged from 1.4% to 8.2% and 0.9% to 3.4%, respectively. Our results indicate that the method is sensitive, accurate and precise. | en |
dc.rights | restrictedAccess | |
dc.source | Jugoslovenska medicinska biohemija | |
dc.subject | Estimation | en |
dc.subject | HI-6 | en |
dc.subject | HPLC method | en |
dc.subject | Trimedoxine | en |
dc.title | HPLC method for determination of oximes HI-6 and trimedoxime in plasma | en |
dc.title | HPLC metoda za odredivanje oksima hi-6 i trimedoksima u plazmi | sr |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Милић, Б; Ђукић, Мирјана; Недељковић, М; ХПЛЦ метода за одредивање оксима хи-6 и тримедоксима у плазми; ХПЛЦ метода за одредивање оксима хи-6 и тримедоксима у плазми; | |
dc.citation.volume | 14 | |
dc.citation.issue | 3-4 | |
dc.citation.spage | 125 | |
dc.citation.epage | 129 | |
dc.citation.other | 14(3-4): 125-129 | |
dc.identifier.scopus | 2-s2.0-0029558212 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_farfar_124 | |
dc.type.version | publishedVersion |
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