Determination of Moclobemide and its Metabolites in Human Plasma by SPE-HPLC-UV: Evaluation of Critical Experimental Conditions and QSRR Study

Abstract

A SPE-HPLC-UV method for determination of moclobemide and its major metabolites, Ro 12-5637 and Ro 12-8095, in human plasma had been developed previously and its selectivity was evaluated against the most frequently coadministered drugs. The objective of the present work was to develop a quantitative structure retention relationship (QSRR) model capable of providing good predictions of chromatographic behaviour of other related potentially interfering drugs, based on the previously generated data, in order to further improve the clinical applicability of the existing method. Moreover, the most critical factors affecting SPE and chromatographic separations of moclobemide and its metabolites were evaluated and discussed, taking into account molecular properties of the analyzed compounds.