Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization
Abstract
A method for separation of three antidepressants, maprotiline, desipramine, and moclobemide, by reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated. To find optimal conditions and estimate the impact of individual parameters on the separation, a complete set of 23 interdependent relationships of the mobile phase composition, temperature, and the volume flow rate were examined. Full separation of the investigated components from a laboratory mixture was achieved on a Supelcosil LC-18 (120mmx4.6mm, 5 mu m) column, using two solvent systems, 3% ammonium ion in water/ethanol and acetonitrile, and alternating isocratic gradient-isocratic elution modes. Relevance of the proposed method for therapeutic drug monitoring is anticipated.
Keywords:
Antidepressants / desipramine / maprotiline / moclobemide / pharmaceutical formulations / RP-HPLC / statistical optimizationSource:
Analytical Letters, 2009, 42, 13, 2060-2070Publisher:
- Taylor & Francis Inc, Philadelphia
Funding / projects:
DOI: 10.1080/00032710903082747
ISSN: 0003-2719
WoS: 000269770400010
Scopus: 2-s2.0-70350005086
Collections
Institution/Community
PharmacyTY - JOUR AU - Vujić, Zorica AU - Uskoković-Marković, Snežana AU - Kuntić, Vesna PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1266 AB - A method for separation of three antidepressants, maprotiline, desipramine, and moclobemide, by reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated. To find optimal conditions and estimate the impact of individual parameters on the separation, a complete set of 23 interdependent relationships of the mobile phase composition, temperature, and the volume flow rate were examined. Full separation of the investigated components from a laboratory mixture was achieved on a Supelcosil LC-18 (120mmx4.6mm, 5 mu m) column, using two solvent systems, 3% ammonium ion in water/ethanol and acetonitrile, and alternating isocratic gradient-isocratic elution modes. Relevance of the proposed method for therapeutic drug monitoring is anticipated. PB - Taylor & Francis Inc, Philadelphia T2 - Analytical Letters T1 - Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization VL - 42 IS - 13 SP - 2060 EP - 2070 DO - 10.1080/00032710903082747 ER -
@article{ author = "Vujić, Zorica and Uskoković-Marković, Snežana and Kuntić, Vesna", year = "2009", abstract = "A method for separation of three antidepressants, maprotiline, desipramine, and moclobemide, by reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated. To find optimal conditions and estimate the impact of individual parameters on the separation, a complete set of 23 interdependent relationships of the mobile phase composition, temperature, and the volume flow rate were examined. Full separation of the investigated components from a laboratory mixture was achieved on a Supelcosil LC-18 (120mmx4.6mm, 5 mu m) column, using two solvent systems, 3% ammonium ion in water/ethanol and acetonitrile, and alternating isocratic gradient-isocratic elution modes. Relevance of the proposed method for therapeutic drug monitoring is anticipated.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Analytical Letters", title = "Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization", volume = "42", number = "13", pages = "2060-2070", doi = "10.1080/00032710903082747" }
Vujić, Z., Uskoković-Marković, S.,& Kuntić, V.. (2009). Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization. in Analytical Letters Taylor & Francis Inc, Philadelphia., 42(13), 2060-2070. https://doi.org/10.1080/00032710903082747
Vujić Z, Uskoković-Marković S, Kuntić V. Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization. in Analytical Letters. 2009;42(13):2060-2070. doi:10.1080/00032710903082747 .
Vujić, Zorica, Uskoković-Marković, Snežana, Kuntić, Vesna, "Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization" in Analytical Letters, 42, no. 13 (2009):2060-2070, https://doi.org/10.1080/00032710903082747 . .