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Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor
dc.creator | Sajc, L | |
dc.creator | Vunjak-Novaković, G | |
dc.creator | Grubišić, Dragoljub | |
dc.creator | Kovačević, Nada | |
dc.creator | Vuković, D | |
dc.creator | Bugarski, Branko | |
dc.date.accessioned | 2019-09-02T10:49:06Z | |
dc.date.available | 2019-09-02T10:49:06Z | |
dc.date.issued | 1995 | |
dc.identifier.issn | 0175-7598 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/126 | |
dc.description.abstract | The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture. | en |
dc.rights | restrictedAccess | |
dc.source | Applied Microbiology and Biotechnology | |
dc.title | Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Вуњак-Новаковић, Г; Сајц, Л; Грубишић, Драгољуб; Ковачевић, Нада; Вуковић, Д; Бугарски, Бранко; | |
dc.citation.volume | 43 | |
dc.citation.issue | 3 | |
dc.citation.spage | 416 | |
dc.citation.epage | 423 | |
dc.citation.other | 43(3): 416-423 | |
dc.identifier.doi | 10.1007/BF00218443 | |
dc.identifier.scopus | 2-s2.0-0029164342 | |
dc.type.version | publishedVersion |