Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro
Само за регистроване кориснике
2009
Аутори
Bufan, BiljanaMojsilović, Slavko
Božić, Dragana
Vucević, Dragana
Vasilijić, Saša
Balint, Bela
Čolić, Miodrag
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Acetylsalicilyc acid (aspirin, ASA) is a well known anti-inflammatory drug with immunomodulatory properties. NO-releasing aspirins (NO-ASA) are new compounds with anti-inflammatory properties. We studied the effects of ASA and two NO-ASA (NCX 4016 and NCX 4040) on human monocyte-derived dendritic cells (MoDC). Immature MoDC were generated in vitro from monocytes in the presence of recombinant human granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4. Mature MoDC were obtained by adding lipopolysaccharide (LPS) in cultures of immature MoDC. As we found that ASA at 4-8 mM, NCX 4016 at 400-800 mu M and NCX 4040 at 4-8 mu M stimulated apoptosis of monocytes and immature MoDC, sub-apoptotic concentrations of ASA (2 mM), NCX 4016 (200 mu M) and NCX 4040 (2 mu M) were used in experiments. Examined substances were added at the beginning of MoDC cultivation. MoDC differentiated in the presence of examined compounds had lower expression of HLA-DR, CD80, CD40 and CD54..., decreased allostimulatory activity and lower production of IL-12 p40. ASA and NCX 4016 decreased production of IL-10, whereas NCX 4040 had the opposite effect. ASA inhibited the expression of CD1a and prevented downregulation of 014, NCX 4016 stimulated the differentiation of CD1a(+)CD14(+) and CD1a(-)CD14(+) cells, whereas NCX 4040 decreased the proportion of CD1a(+)CD14(-) and increased the frequency of CD1a(+)CD14(+) cells, compared to control. Maturation, both in ASA and NO-ASA treated MoDC was characterized by decreased allostimulatory activity, lower expression of CD83, HLA-DR, costimulatory molecules and CD54 and decreased production of IL-10 and IL-12 p40. In conclusion, we confirmed that ASA impairs differentiation, maturation and function of MoDC and found that NCX 4016 and NCX 4040 exerted similar, but not identical effects at about 10- and 1000-fold lower concentrations, respectively, compared to ASA.
Кључне речи:
Human monocyte-derived dendritic cells / Aspirin / NO-releasing aspirins / Differentiation / MaturationИзвор:
International Immunopharmacology, 2009, 9, 7-8, 910-917Издавач:
- Elsevier Science BV, Amsterdam
DOI: 10.1016/j.intimp.2009.03.016
ISSN: 1567-5769
PubMed: 19341823
WoS: 000267444800015
Scopus: 2-s2.0-67349165024
Институција/група
PharmacyTY - JOUR AU - Bufan, Biljana AU - Mojsilović, Slavko AU - Božić, Dragana AU - Vucević, Dragana AU - Vasilijić, Saša AU - Balint, Bela AU - Čolić, Miodrag PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1276 AB - Acetylsalicilyc acid (aspirin, ASA) is a well known anti-inflammatory drug with immunomodulatory properties. NO-releasing aspirins (NO-ASA) are new compounds with anti-inflammatory properties. We studied the effects of ASA and two NO-ASA (NCX 4016 and NCX 4040) on human monocyte-derived dendritic cells (MoDC). Immature MoDC were generated in vitro from monocytes in the presence of recombinant human granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4. Mature MoDC were obtained by adding lipopolysaccharide (LPS) in cultures of immature MoDC. As we found that ASA at 4-8 mM, NCX 4016 at 400-800 mu M and NCX 4040 at 4-8 mu M stimulated apoptosis of monocytes and immature MoDC, sub-apoptotic concentrations of ASA (2 mM), NCX 4016 (200 mu M) and NCX 4040 (2 mu M) were used in experiments. Examined substances were added at the beginning of MoDC cultivation. MoDC differentiated in the presence of examined compounds had lower expression of HLA-DR, CD80, CD40 and CD54, decreased allostimulatory activity and lower production of IL-12 p40. ASA and NCX 4016 decreased production of IL-10, whereas NCX 4040 had the opposite effect. ASA inhibited the expression of CD1a and prevented downregulation of 014, NCX 4016 stimulated the differentiation of CD1a(+)CD14(+) and CD1a(-)CD14(+) cells, whereas NCX 4040 decreased the proportion of CD1a(+)CD14(-) and increased the frequency of CD1a(+)CD14(+) cells, compared to control. Maturation, both in ASA and NO-ASA treated MoDC was characterized by decreased allostimulatory activity, lower expression of CD83, HLA-DR, costimulatory molecules and CD54 and decreased production of IL-10 and IL-12 p40. In conclusion, we confirmed that ASA impairs differentiation, maturation and function of MoDC and found that NCX 4016 and NCX 4040 exerted similar, but not identical effects at about 10- and 1000-fold lower concentrations, respectively, compared to ASA. PB - Elsevier Science BV, Amsterdam T2 - International Immunopharmacology T1 - Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro VL - 9 IS - 7-8 SP - 910 EP - 917 DO - 10.1016/j.intimp.2009.03.016 ER -
@article{ author = "Bufan, Biljana and Mojsilović, Slavko and Božić, Dragana and Vucević, Dragana and Vasilijić, Saša and Balint, Bela and Čolić, Miodrag", year = "2009", abstract = "Acetylsalicilyc acid (aspirin, ASA) is a well known anti-inflammatory drug with immunomodulatory properties. NO-releasing aspirins (NO-ASA) are new compounds with anti-inflammatory properties. We studied the effects of ASA and two NO-ASA (NCX 4016 and NCX 4040) on human monocyte-derived dendritic cells (MoDC). Immature MoDC were generated in vitro from monocytes in the presence of recombinant human granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4. Mature MoDC were obtained by adding lipopolysaccharide (LPS) in cultures of immature MoDC. As we found that ASA at 4-8 mM, NCX 4016 at 400-800 mu M and NCX 4040 at 4-8 mu M stimulated apoptosis of monocytes and immature MoDC, sub-apoptotic concentrations of ASA (2 mM), NCX 4016 (200 mu M) and NCX 4040 (2 mu M) were used in experiments. Examined substances were added at the beginning of MoDC cultivation. MoDC differentiated in the presence of examined compounds had lower expression of HLA-DR, CD80, CD40 and CD54, decreased allostimulatory activity and lower production of IL-12 p40. ASA and NCX 4016 decreased production of IL-10, whereas NCX 4040 had the opposite effect. ASA inhibited the expression of CD1a and prevented downregulation of 014, NCX 4016 stimulated the differentiation of CD1a(+)CD14(+) and CD1a(-)CD14(+) cells, whereas NCX 4040 decreased the proportion of CD1a(+)CD14(-) and increased the frequency of CD1a(+)CD14(+) cells, compared to control. Maturation, both in ASA and NO-ASA treated MoDC was characterized by decreased allostimulatory activity, lower expression of CD83, HLA-DR, costimulatory molecules and CD54 and decreased production of IL-10 and IL-12 p40. In conclusion, we confirmed that ASA impairs differentiation, maturation and function of MoDC and found that NCX 4016 and NCX 4040 exerted similar, but not identical effects at about 10- and 1000-fold lower concentrations, respectively, compared to ASA.", publisher = "Elsevier Science BV, Amsterdam", journal = "International Immunopharmacology", title = "Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro", volume = "9", number = "7-8", pages = "910-917", doi = "10.1016/j.intimp.2009.03.016" }
Bufan, B., Mojsilović, S., Božić, D., Vucević, D., Vasilijić, S., Balint, B.,& Čolić, M.. (2009). Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro. in International Immunopharmacology Elsevier Science BV, Amsterdam., 9(7-8), 910-917. https://doi.org/10.1016/j.intimp.2009.03.016
Bufan B, Mojsilović S, Božić D, Vucević D, Vasilijić S, Balint B, Čolić M. Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro. in International Immunopharmacology. 2009;9(7-8):910-917. doi:10.1016/j.intimp.2009.03.016 .
Bufan, Biljana, Mojsilović, Slavko, Božić, Dragana, Vucević, Dragana, Vasilijić, Saša, Balint, Bela, Čolić, Miodrag, "Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro" in International Immunopharmacology, 9, no. 7-8 (2009):910-917, https://doi.org/10.1016/j.intimp.2009.03.016 . .