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dc.creatorBufan, Biljana
dc.creatorMojsilović, Slavko
dc.creatorBožić, Dragana
dc.creatorVucević, Dragana
dc.creatorVasilijić, Saša
dc.creatorBalint, Bela
dc.creatorČolić, Miodrag
dc.date.accessioned2019-09-02T11:18:43Z
dc.date.available2019-09-02T11:18:43Z
dc.date.issued2009
dc.identifier.issn1567-5769
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1276
dc.description.abstractAcetylsalicilyc acid (aspirin, ASA) is a well known anti-inflammatory drug with immunomodulatory properties. NO-releasing aspirins (NO-ASA) are new compounds with anti-inflammatory properties. We studied the effects of ASA and two NO-ASA (NCX 4016 and NCX 4040) on human monocyte-derived dendritic cells (MoDC). Immature MoDC were generated in vitro from monocytes in the presence of recombinant human granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4. Mature MoDC were obtained by adding lipopolysaccharide (LPS) in cultures of immature MoDC. As we found that ASA at 4-8 mM, NCX 4016 at 400-800 mu M and NCX 4040 at 4-8 mu M stimulated apoptosis of monocytes and immature MoDC, sub-apoptotic concentrations of ASA (2 mM), NCX 4016 (200 mu M) and NCX 4040 (2 mu M) were used in experiments. Examined substances were added at the beginning of MoDC cultivation. MoDC differentiated in the presence of examined compounds had lower expression of HLA-DR, CD80, CD40 and CD54, decreased allostimulatory activity and lower production of IL-12 p40. ASA and NCX 4016 decreased production of IL-10, whereas NCX 4040 had the opposite effect. ASA inhibited the expression of CD1a and prevented downregulation of 014, NCX 4016 stimulated the differentiation of CD1a(+)CD14(+) and CD1a(-)CD14(+) cells, whereas NCX 4040 decreased the proportion of CD1a(+)CD14(-) and increased the frequency of CD1a(+)CD14(+) cells, compared to control. Maturation, both in ASA and NO-ASA treated MoDC was characterized by decreased allostimulatory activity, lower expression of CD83, HLA-DR, costimulatory molecules and CD54 and decreased production of IL-10 and IL-12 p40. In conclusion, we confirmed that ASA impairs differentiation, maturation and function of MoDC and found that NCX 4016 and NCX 4040 exerted similar, but not identical effects at about 10- and 1000-fold lower concentrations, respectively, compared to ASA.en
dc.publisherElsevier Science BV, Amsterdam
dc.rightsrestrictedAccess
dc.sourceInternational Immunopharmacology
dc.subjectHuman monocyte-derived dendritic cellsen
dc.subjectAspirinen
dc.subjectNO-releasing aspirinsen
dc.subjectDifferentiationen
dc.subjectMaturationen
dc.titleComparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitroen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВуцевић, Драгана; Буфан, Биљана; Мојсиловић, Славко; Божић, Драгана; Чолић, Миодраг; Балинт, Бела; Василијић, Саша;
dc.citation.volume9
dc.citation.issue7-8
dc.citation.spage910
dc.citation.epage917
dc.citation.other9(7-8): 910-917
dc.citation.rankM22
dc.identifier.wos000267444800015
dc.identifier.doi10.1016/j.intimp.2009.03.016
dc.identifier.pmid19341823
dc.identifier.scopus2-s2.0-67349165024
dc.identifier.rcubconv_2166
dc.type.versionpublishedVersion


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