Normal-phase thin-layer chromatography of some angiotensin converting enzyme (ACE) inhibitors and their metabolites
Normalno-fazna tankoslojna hromatografija nekih ACE inhibitora i njihovih metabolita

2009
Authors
Odović, Jadranka
Aleksić, Mirjana
Stojimirović, Biljana
Milojković-Opsenica, Dušanka

Tešić, Živoslav

Article (Published version)
Metadata
Show full item recordAbstract
The separation and chromatographic behaviour of five ACE (angiotensin converting enzyme) inhibitors and their four active metabolites were investigated by normal-phase thin-layer chromatography on silica using several mono- and binary non-aqueous solvent systems. The linear relationship between the RM values and the composition of employed mobile phase was obtained. The hydrophobicity parameters 0M R and C0 were determined from the regression data of the plots, analogous to reversed-phase chromatography. The chromatographically obtained hydrophobicity parameters were correlated with the calculated log P values. The current results were correlated with the lipophilicity of the studied ACE inhibitors and their metabolites, previously estimated by reversed-phase chromatography.
Hromatografsko razdvajanje I ponašanje pet ASE inhibitora (inhibitora angio-tenzin-konvertujućeg enzima) I njihova četiri aktivna metabolita ispitivano je metodom normalno-fazne tankoslojne hromatografije na silika-gelu primenom nekoliko jedno- I dvo-komponentnih nevodenih sistema rastvarača. Dobijena je linearna zavisnost između RM-vrednosti I koncentracije etanola u mobilnoj fazi. Na osnovu odgovarajućih regresionih podataka, po analogiji sa reverzno-faznom hromatografijom, izračunati su parametri lipofilnosti 0M R I C0. Hromatografski dobijeni parametri hidrofobnosti korelisani su sa izračunatim log P vrednostima. Takođe, 0M R -vrednosti dobijene u ovom radu korelisane su sa 0M R -vrednostima dobijenim metodom reverzno-fazne tankoslojne hromatografije.
Keywords:
ACE inhibitors / normal-phase thin-layer chromatography / hydrophobicitySource:
Journal of the Serbian Chemical Society, 2009, 74, 6, 677-688Publisher:
- Srpsko hemijsko društvo, Beograd
Projects:
- Sinteza, analiza i aktivnost novih organskih polidentatnih liganada i njihovih kompleksa sa d-metalima (RS-142062)
DOI: 10.2298/JSC0906677O
ISSN: 0352-5139