Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application
Imunonefelometrija i imunoturbidimetrija - teorijski principi, instrumenti i primena
Apstrakt
Nephelometry and turbidimetry are methods based on the phenomenon whereby light, passing through a medium with dispersed particles is attenuated in intensity by scattering. The intensity of the scattered light is measured at right angle (usually, but not necessarily) to the incident light beam (nephelometry) or at a forward angle (turbidimetry). Based on nephelometry and turbidimetry, immunonephelometric and immunoturbidimetric assays for the measurement of proteins have developed and expanded rapidly in recent years, progressively replacing the time-honored gel precipitation techniques. In this study, the theoretical background of light scattering theory, instrumental systems for nephelometry and turbidimetry, the nature and kinetics of the antibody-antigen reaction in fluid, and the application of immunonephelometric and immunoturbidimetric assays for specific proteins in the clinical laboratory are described.
Nefelometrija i turbidimetrija su metode koje sa zasnivaju na rasipanju svetlosti: pri prolasku kroz disperzionu sredinu, svetlost se rasipa na česticama disperzione faze. Intenzitet rasute svetlosti može se meriti pod nekim uglom (uobičajeno, ali ne i obavezno, pod pravim uglom), što predstavlja nefelometriju ili u pravcu upadne svetlosti, što predstavlja turbidimetriju. Primenom nefelometrije i turbidimetrije u imunologiji, razvile su se imunonefelometrija i imunoturbidimetrija, kao savremene tehnike za određivanje proteina i peptida, koje su zamenile klasične, dugotrajne, precipitacione tehnike u gelu. U ovom radu objašnjene su teorijske osnove rasipanja svetlosti, prikazane su sheme i delovi instrumenata nefelometra i turbidimetra i opisane su imunonefelometrija i imunoturbidimetrija koje su u širokoj upotrebi u savremenim kliničkim laboratorijama.
Ključne reči:
nephelometry / turbidimetry / scattered light / immunonephelometric and immunoturbidimetric assays / nefelometrija / turbidimetrija / rasuta svetlost / imunonefelometrijski i imunoturbidimetrijski testoviIzvor:
Arhiv za farmaciju, 2009, 59, 6, 536-550Izdavač:
- Savez farmaceutskih udruženja Srbije, Beograd
Institucija/grupa
PharmacyTY - JOUR AU - Kuntić, Vesna AU - Stojić-Vukanić, Zorica PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1308 AB - Nephelometry and turbidimetry are methods based on the phenomenon whereby light, passing through a medium with dispersed particles is attenuated in intensity by scattering. The intensity of the scattered light is measured at right angle (usually, but not necessarily) to the incident light beam (nephelometry) or at a forward angle (turbidimetry). Based on nephelometry and turbidimetry, immunonephelometric and immunoturbidimetric assays for the measurement of proteins have developed and expanded rapidly in recent years, progressively replacing the time-honored gel precipitation techniques. In this study, the theoretical background of light scattering theory, instrumental systems for nephelometry and turbidimetry, the nature and kinetics of the antibody-antigen reaction in fluid, and the application of immunonephelometric and immunoturbidimetric assays for specific proteins in the clinical laboratory are described. AB - Nefelometrija i turbidimetrija su metode koje sa zasnivaju na rasipanju svetlosti: pri prolasku kroz disperzionu sredinu, svetlost se rasipa na česticama disperzione faze. Intenzitet rasute svetlosti može se meriti pod nekim uglom (uobičajeno, ali ne i obavezno, pod pravim uglom), što predstavlja nefelometriju ili u pravcu upadne svetlosti, što predstavlja turbidimetriju. Primenom nefelometrije i turbidimetrije u imunologiji, razvile su se imunonefelometrija i imunoturbidimetrija, kao savremene tehnike za određivanje proteina i peptida, koje su zamenile klasične, dugotrajne, precipitacione tehnike u gelu. U ovom radu objašnjene su teorijske osnove rasipanja svetlosti, prikazane su sheme i delovi instrumenata nefelometra i turbidimetra i opisane su imunonefelometrija i imunoturbidimetrija koje su u širokoj upotrebi u savremenim kliničkim laboratorijama. PB - Savez farmaceutskih udruženja Srbije, Beograd T2 - Arhiv za farmaciju T1 - Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application T1 - Imunonefelometrija i imunoturbidimetrija - teorijski principi, instrumenti i primena VL - 59 IS - 6 SP - 536 EP - 550 UR - https://hdl.handle.net/21.15107/rcub_farfar_1308 ER -
@article{ author = "Kuntić, Vesna and Stojić-Vukanić, Zorica", year = "2009", abstract = "Nephelometry and turbidimetry are methods based on the phenomenon whereby light, passing through a medium with dispersed particles is attenuated in intensity by scattering. The intensity of the scattered light is measured at right angle (usually, but not necessarily) to the incident light beam (nephelometry) or at a forward angle (turbidimetry). Based on nephelometry and turbidimetry, immunonephelometric and immunoturbidimetric assays for the measurement of proteins have developed and expanded rapidly in recent years, progressively replacing the time-honored gel precipitation techniques. In this study, the theoretical background of light scattering theory, instrumental systems for nephelometry and turbidimetry, the nature and kinetics of the antibody-antigen reaction in fluid, and the application of immunonephelometric and immunoturbidimetric assays for specific proteins in the clinical laboratory are described., Nefelometrija i turbidimetrija su metode koje sa zasnivaju na rasipanju svetlosti: pri prolasku kroz disperzionu sredinu, svetlost se rasipa na česticama disperzione faze. Intenzitet rasute svetlosti može se meriti pod nekim uglom (uobičajeno, ali ne i obavezno, pod pravim uglom), što predstavlja nefelometriju ili u pravcu upadne svetlosti, što predstavlja turbidimetriju. Primenom nefelometrije i turbidimetrije u imunologiji, razvile su se imunonefelometrija i imunoturbidimetrija, kao savremene tehnike za određivanje proteina i peptida, koje su zamenile klasične, dugotrajne, precipitacione tehnike u gelu. U ovom radu objašnjene su teorijske osnove rasipanja svetlosti, prikazane su sheme i delovi instrumenata nefelometra i turbidimetra i opisane su imunonefelometrija i imunoturbidimetrija koje su u širokoj upotrebi u savremenim kliničkim laboratorijama.", publisher = "Savez farmaceutskih udruženja Srbije, Beograd", journal = "Arhiv za farmaciju", title = "Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application, Imunonefelometrija i imunoturbidimetrija - teorijski principi, instrumenti i primena", volume = "59", number = "6", pages = "536-550", url = "https://hdl.handle.net/21.15107/rcub_farfar_1308" }
Kuntić, V.,& Stojić-Vukanić, Z.. (2009). Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application. in Arhiv za farmaciju Savez farmaceutskih udruženja Srbije, Beograd., 59(6), 536-550. https://hdl.handle.net/21.15107/rcub_farfar_1308
Kuntić V, Stojić-Vukanić Z. Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application. in Arhiv za farmaciju. 2009;59(6):536-550. https://hdl.handle.net/21.15107/rcub_farfar_1308 .
Kuntić, Vesna, Stojić-Vukanić, Zorica, "Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application" in Arhiv za farmaciju, 59, no. 6 (2009):536-550, https://hdl.handle.net/21.15107/rcub_farfar_1308 .