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Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats

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2010
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Аутори
Milenković, Marina
Arsenović-Ranin, Nevena
Stojić-Vukanić, Zorica
Bufan, Biljana
Vučićević, Dragana
Jančić, Ivan
Чланак у часопису (Објављена верзија)
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Апстракт
Purpose. Experimental autoimmune myocarditis (EAM) in rats is an animal model of human giant cell myocarditis and post-myocarditis dilated cardiomyopathy. The pathogenesis of EAM has not been elucidated, but there is accumulating evidence that cytokines secreted from monocytes/macrophages and T cells play a crucial role in the induction and progression of disease. Flavonoids are a large group of polyphenolic compounds abundantly present in the human diet, which scavenge oxygen radicals and have anti-inflammatory activities. Having in mind in vivo beneficial effects of flavonoid quercetin in different animal models of immunoinflammatory diseases such as experimental autoimmune encephalomyelitis and adjuvant arthritis, on the one side, and its in vitro suppressive effect on production of tumor necrosis factor-alpha (TNF-alpha), on the other side, we investigated the effects of quercetin on EAM in rats. Methods. Myocarditis was induced in Dark Agouti (DA) rats by injection of porcine card...iac myosin and quercetin at doses of 10 or 20 mg/kg was orally administered from days 0 to 21 after induction of disease. The severity of myocarditis was evaluated by determination of heart weight/body weight ratio (Hw/Bw) and histopathological examination of hearts. The levels of cytokines (TNF-alpha, IL-12, IL-17 and IL-10) in serum and lymph node cells (LNC) culture supernatants were measured by ELISA. Results. The rats treated with 20 mg/kg of quercetin had significantly decreased incidence of EAM, Hw/Bw, macroscopic and microscopic scores of hearts. Further, in EAM rats treated with quercetin levels of TNF-alpha and IL-17 were significantly lower, while the level of IL-10 was significantly higher both in serum and culture supernatants of LNC stimulated with concanavalin A compared with vehicle-treated animals. Conclusions. The present study suggests that quercetin ameliorates EAM, at least in part, by interfering production of proinflammatory (TNF-alpha and IL-17) and/or anti-inflammatory (IL-10) cytokines.

Извор:
Journal of Pharmacy and Pharmaceutical Sciences, 2010, 13, 3, 311-319
Издавач:
  • Canadian Soc Pharmaceutical Sciences, Edmonton

DOI: 10.18433/J3VS3S

ISSN: 1482-1826

PubMed: 21092705

WoS: 000286930600001

Scopus: 2-s2.0-78650400220
[ Google Scholar ]
34
31
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1327
Колекције
  • Radovi istraživača / Researchers’ publications
Институција
Pharmacy
TY  - JOUR
AU  - Milenković, Marina
AU  - Arsenović-Ranin, Nevena
AU  - Stojić-Vukanić, Zorica
AU  - Bufan, Biljana
AU  - Vučićević, Dragana
AU  - Jančić, Ivan
PY  - 2010
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1327
AB  - Purpose. Experimental autoimmune myocarditis (EAM) in rats is an animal model of human giant cell myocarditis and post-myocarditis dilated cardiomyopathy. The pathogenesis of EAM has not been elucidated, but there is accumulating evidence that cytokines secreted from monocytes/macrophages and T cells play a crucial role in the induction and progression of disease. Flavonoids are a large group of polyphenolic compounds abundantly present in the human diet, which scavenge oxygen radicals and have anti-inflammatory activities. Having in mind in vivo beneficial effects of flavonoid quercetin in different animal models of immunoinflammatory diseases such as experimental autoimmune encephalomyelitis and adjuvant arthritis, on the one side, and its in vitro suppressive effect on production of tumor necrosis factor-alpha (TNF-alpha), on the other side, we investigated the effects of quercetin on EAM in rats. Methods. Myocarditis was induced in Dark Agouti (DA) rats by injection of porcine cardiac myosin and quercetin at doses of 10 or 20 mg/kg was orally administered from days 0 to 21 after induction of disease. The severity of myocarditis was evaluated by determination of heart weight/body weight ratio (Hw/Bw) and histopathological examination of hearts. The levels of cytokines (TNF-alpha, IL-12, IL-17 and IL-10) in serum and lymph node cells (LNC) culture supernatants were measured by ELISA. Results. The rats treated with 20 mg/kg of quercetin had significantly decreased incidence of EAM, Hw/Bw, macroscopic and microscopic scores of hearts. Further, in EAM rats treated with quercetin levels of TNF-alpha and IL-17 were significantly lower, while the level of IL-10 was significantly higher both in serum and culture supernatants of LNC stimulated with concanavalin A compared with vehicle-treated animals. Conclusions. The present study suggests that quercetin ameliorates EAM, at least in part, by interfering production of proinflammatory (TNF-alpha and IL-17) and/or anti-inflammatory (IL-10) cytokines.
PB  - Canadian Soc Pharmaceutical Sciences, Edmonton
T2  - Journal of Pharmacy and Pharmaceutical Sciences
T1  - Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats
VL  - 13
IS  - 3
SP  - 311
EP  - 319
DO  - 10.18433/J3VS3S
ER  - 
@article{
author = "Milenković, Marina and Arsenović-Ranin, Nevena and Stojić-Vukanić, Zorica and Bufan, Biljana and Vučićević, Dragana and Jančić, Ivan",
year = "2010",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/1327",
abstract = "Purpose. Experimental autoimmune myocarditis (EAM) in rats is an animal model of human giant cell myocarditis and post-myocarditis dilated cardiomyopathy. The pathogenesis of EAM has not been elucidated, but there is accumulating evidence that cytokines secreted from monocytes/macrophages and T cells play a crucial role in the induction and progression of disease. Flavonoids are a large group of polyphenolic compounds abundantly present in the human diet, which scavenge oxygen radicals and have anti-inflammatory activities. Having in mind in vivo beneficial effects of flavonoid quercetin in different animal models of immunoinflammatory diseases such as experimental autoimmune encephalomyelitis and adjuvant arthritis, on the one side, and its in vitro suppressive effect on production of tumor necrosis factor-alpha (TNF-alpha), on the other side, we investigated the effects of quercetin on EAM in rats. Methods. Myocarditis was induced in Dark Agouti (DA) rats by injection of porcine cardiac myosin and quercetin at doses of 10 or 20 mg/kg was orally administered from days 0 to 21 after induction of disease. The severity of myocarditis was evaluated by determination of heart weight/body weight ratio (Hw/Bw) and histopathological examination of hearts. The levels of cytokines (TNF-alpha, IL-12, IL-17 and IL-10) in serum and lymph node cells (LNC) culture supernatants were measured by ELISA. Results. The rats treated with 20 mg/kg of quercetin had significantly decreased incidence of EAM, Hw/Bw, macroscopic and microscopic scores of hearts. Further, in EAM rats treated with quercetin levels of TNF-alpha and IL-17 were significantly lower, while the level of IL-10 was significantly higher both in serum and culture supernatants of LNC stimulated with concanavalin A compared with vehicle-treated animals. Conclusions. The present study suggests that quercetin ameliorates EAM, at least in part, by interfering production of proinflammatory (TNF-alpha and IL-17) and/or anti-inflammatory (IL-10) cytokines.",
publisher = "Canadian Soc Pharmaceutical Sciences, Edmonton",
journal = "Journal of Pharmacy and Pharmaceutical Sciences",
title = "Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats",
volume = "13",
number = "3",
pages = "311-319",
doi = "10.18433/J3VS3S"
}
Milenković M, Arsenović-Ranin N, Stojić-Vukanić Z, Bufan B, Vučićević D, Jančić I. Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats. Journal of Pharmacy and Pharmaceutical Sciences. 2010;13(3):311-319
Milenković, M., Arsenović-Ranin, N., Stojić-Vukanić, Z., Bufan, B., Vučićević, D.,& Jančić, I. (2010). Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats.
Journal of Pharmacy and Pharmaceutical SciencesCanadian Soc Pharmaceutical Sciences, Edmonton., 13(3), 311-319.
https://doi.org/10.18433/J3VS3S
Milenković Marina, Arsenović-Ranin Nevena, Stojić-Vukanić Zorica, Bufan Biljana, Vučićević Dragana, Jančić Ivan, "Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats" 13, no. 3 (2010):311-319,
https://doi.org/10.18433/J3VS3S .

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