Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats
2010
Аутори
Milenković, MarinaArsenović-Ranin, Nevena
Stojić-Vukanić, Zorica
Bufan, Biljana
Vučićević, Dragana
Jančić, Ivan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Purpose. Experimental autoimmune myocarditis (EAM) in rats is an animal model of human giant cell myocarditis and post-myocarditis dilated cardiomyopathy. The pathogenesis of EAM has not been elucidated, but there is accumulating evidence that cytokines secreted from monocytes/macrophages and T cells play a crucial role in the induction and progression of disease. Flavonoids are a large group of polyphenolic compounds abundantly present in the human diet, which scavenge oxygen radicals and have anti-inflammatory activities. Having in mind in vivo beneficial effects of flavonoid quercetin in different animal models of immunoinflammatory diseases such as experimental autoimmune encephalomyelitis and adjuvant arthritis, on the one side, and its in vitro suppressive effect on production of tumor necrosis factor-alpha (TNF-alpha), on the other side, we investigated the effects of quercetin on EAM in rats. Methods. Myocarditis was induced in Dark Agouti (DA) rats by injection of porcine card...iac myosin and quercetin at doses of 10 or 20 mg/kg was orally administered from days 0 to 21 after induction of disease. The severity of myocarditis was evaluated by determination of heart weight/body weight ratio (Hw/Bw) and histopathological examination of hearts. The levels of cytokines (TNF-alpha, IL-12, IL-17 and IL-10) in serum and lymph node cells (LNC) culture supernatants were measured by ELISA. Results. The rats treated with 20 mg/kg of quercetin had significantly decreased incidence of EAM, Hw/Bw, macroscopic and microscopic scores of hearts. Further, in EAM rats treated with quercetin levels of TNF-alpha and IL-17 were significantly lower, while the level of IL-10 was significantly higher both in serum and culture supernatants of LNC stimulated with concanavalin A compared with vehicle-treated animals. Conclusions. The present study suggests that quercetin ameliorates EAM, at least in part, by interfering production of proinflammatory (TNF-alpha and IL-17) and/or anti-inflammatory (IL-10) cytokines.
Извор:
Journal of Pharmacy and Pharmaceutical Sciences, 2010, 13, 3, 311-319Издавач:
- Canadian Soc Pharmaceutical Sciences, Edmonton
DOI: 10.18433/J3VS3S
ISSN: 1482-1826
PubMed: 21092705
WoS: 000286930600001
Scopus: 2-s2.0-78650400220
Институција/група
PharmacyTY - JOUR AU - Milenković, Marina AU - Arsenović-Ranin, Nevena AU - Stojić-Vukanić, Zorica AU - Bufan, Biljana AU - Vučićević, Dragana AU - Jančić, Ivan PY - 2010 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1327 AB - Purpose. Experimental autoimmune myocarditis (EAM) in rats is an animal model of human giant cell myocarditis and post-myocarditis dilated cardiomyopathy. The pathogenesis of EAM has not been elucidated, but there is accumulating evidence that cytokines secreted from monocytes/macrophages and T cells play a crucial role in the induction and progression of disease. Flavonoids are a large group of polyphenolic compounds abundantly present in the human diet, which scavenge oxygen radicals and have anti-inflammatory activities. Having in mind in vivo beneficial effects of flavonoid quercetin in different animal models of immunoinflammatory diseases such as experimental autoimmune encephalomyelitis and adjuvant arthritis, on the one side, and its in vitro suppressive effect on production of tumor necrosis factor-alpha (TNF-alpha), on the other side, we investigated the effects of quercetin on EAM in rats. Methods. Myocarditis was induced in Dark Agouti (DA) rats by injection of porcine cardiac myosin and quercetin at doses of 10 or 20 mg/kg was orally administered from days 0 to 21 after induction of disease. The severity of myocarditis was evaluated by determination of heart weight/body weight ratio (Hw/Bw) and histopathological examination of hearts. The levels of cytokines (TNF-alpha, IL-12, IL-17 and IL-10) in serum and lymph node cells (LNC) culture supernatants were measured by ELISA. Results. The rats treated with 20 mg/kg of quercetin had significantly decreased incidence of EAM, Hw/Bw, macroscopic and microscopic scores of hearts. Further, in EAM rats treated with quercetin levels of TNF-alpha and IL-17 were significantly lower, while the level of IL-10 was significantly higher both in serum and culture supernatants of LNC stimulated with concanavalin A compared with vehicle-treated animals. Conclusions. The present study suggests that quercetin ameliorates EAM, at least in part, by interfering production of proinflammatory (TNF-alpha and IL-17) and/or anti-inflammatory (IL-10) cytokines. PB - Canadian Soc Pharmaceutical Sciences, Edmonton T2 - Journal of Pharmacy and Pharmaceutical Sciences T1 - Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats VL - 13 IS - 3 SP - 311 EP - 319 DO - 10.18433/J3VS3S ER -
@article{ author = "Milenković, Marina and Arsenović-Ranin, Nevena and Stojić-Vukanić, Zorica and Bufan, Biljana and Vučićević, Dragana and Jančić, Ivan", year = "2010", abstract = "Purpose. Experimental autoimmune myocarditis (EAM) in rats is an animal model of human giant cell myocarditis and post-myocarditis dilated cardiomyopathy. The pathogenesis of EAM has not been elucidated, but there is accumulating evidence that cytokines secreted from monocytes/macrophages and T cells play a crucial role in the induction and progression of disease. Flavonoids are a large group of polyphenolic compounds abundantly present in the human diet, which scavenge oxygen radicals and have anti-inflammatory activities. Having in mind in vivo beneficial effects of flavonoid quercetin in different animal models of immunoinflammatory diseases such as experimental autoimmune encephalomyelitis and adjuvant arthritis, on the one side, and its in vitro suppressive effect on production of tumor necrosis factor-alpha (TNF-alpha), on the other side, we investigated the effects of quercetin on EAM in rats. Methods. Myocarditis was induced in Dark Agouti (DA) rats by injection of porcine cardiac myosin and quercetin at doses of 10 or 20 mg/kg was orally administered from days 0 to 21 after induction of disease. The severity of myocarditis was evaluated by determination of heart weight/body weight ratio (Hw/Bw) and histopathological examination of hearts. The levels of cytokines (TNF-alpha, IL-12, IL-17 and IL-10) in serum and lymph node cells (LNC) culture supernatants were measured by ELISA. Results. The rats treated with 20 mg/kg of quercetin had significantly decreased incidence of EAM, Hw/Bw, macroscopic and microscopic scores of hearts. Further, in EAM rats treated with quercetin levels of TNF-alpha and IL-17 were significantly lower, while the level of IL-10 was significantly higher both in serum and culture supernatants of LNC stimulated with concanavalin A compared with vehicle-treated animals. Conclusions. The present study suggests that quercetin ameliorates EAM, at least in part, by interfering production of proinflammatory (TNF-alpha and IL-17) and/or anti-inflammatory (IL-10) cytokines.", publisher = "Canadian Soc Pharmaceutical Sciences, Edmonton", journal = "Journal of Pharmacy and Pharmaceutical Sciences", title = "Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats", volume = "13", number = "3", pages = "311-319", doi = "10.18433/J3VS3S" }
Milenković, M., Arsenović-Ranin, N., Stojić-Vukanić, Z., Bufan, B., Vučićević, D.,& Jančić, I.. (2010). Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats. in Journal of Pharmacy and Pharmaceutical Sciences Canadian Soc Pharmaceutical Sciences, Edmonton., 13(3), 311-319. https://doi.org/10.18433/J3VS3S
Milenković M, Arsenović-Ranin N, Stojić-Vukanić Z, Bufan B, Vučićević D, Jančić I. Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats. in Journal of Pharmacy and Pharmaceutical Sciences. 2010;13(3):311-319. doi:10.18433/J3VS3S .
Milenković, Marina, Arsenović-Ranin, Nevena, Stojić-Vukanić, Zorica, Bufan, Biljana, Vučićević, Dragana, Jančić, Ivan, "Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats" in Journal of Pharmacy and Pharmaceutical Sciences, 13, no. 3 (2010):311-319, https://doi.org/10.18433/J3VS3S . .