Zinc binding to P113 (or demegen), a 12 amino acid (AKRHHGYKRKFH-NH2) fragment of histatin 5, was investigated by means of NMR and CD techniques, yielding delineation of the metal binding site and the 3D structure of the complex in water and in DMSO as well. The three His imidazole and the N-terminus nitrogens were found to act as the zinc coordinating atoms. A comparison with the previously reported Cu(II)-P113 complex disclosed that the two structures were rather diverse, in spite of an identical donor set. The two complexes were also tested for their antimicrobial activity in vitro against seven bacteria and two yeast strains: a minor activity of both complexes vs that of free ligand was given evidence, suggesting both metal ions may possibly play a negative role in vivo.
Source:
Inorganic Chemistry, 2010, 49, 19, 8690-8698
Publisher:
Amer Chemical Soc, Washington
Funding / projects:
CIRMMP (Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine Paramagnetiche)
TY - JOUR
AU - Porciatti, Elena
AU - Milenković, Marina
AU - Gaggelli, Elena
AU - Valensin, Gianni
AU - Kozlowski, Henryk
AU - Kamysz, Wojciech
AU - Valensin, Daniela
PY - 2010
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1328
AB - Zinc binding to P113 (or demegen), a 12 amino acid (AKRHHGYKRKFH-NH2) fragment of histatin 5, was investigated by means of NMR and CD techniques, yielding delineation of the metal binding site and the 3D structure of the complex in water and in DMSO as well. The three His imidazole and the N-terminus nitrogens were found to act as the zinc coordinating atoms. A comparison with the previously reported Cu(II)-P113 complex disclosed that the two structures were rather diverse, in spite of an identical donor set. The two complexes were also tested for their antimicrobial activity in vitro against seven bacteria and two yeast strains: a minor activity of both complexes vs that of free ligand was given evidence, suggesting both metal ions may possibly play a negative role in vivo.
PB - Amer Chemical Soc, Washington
T2 - Inorganic Chemistry
T1 - Structural Characterization and Antimicrobial Activity of the Zn(II) Complex with P113 (Demegen), a Derivative of Histatin 5
VL - 49
IS - 19
SP - 8690
EP - 8698
DO - 10.1021/ic902547t
ER -
@article{
author = "Porciatti, Elena and Milenković, Marina and Gaggelli, Elena and Valensin, Gianni and Kozlowski, Henryk and Kamysz, Wojciech and Valensin, Daniela",
year = "2010",
abstract = "Zinc binding to P113 (or demegen), a 12 amino acid (AKRHHGYKRKFH-NH2) fragment of histatin 5, was investigated by means of NMR and CD techniques, yielding delineation of the metal binding site and the 3D structure of the complex in water and in DMSO as well. The three His imidazole and the N-terminus nitrogens were found to act as the zinc coordinating atoms. A comparison with the previously reported Cu(II)-P113 complex disclosed that the two structures were rather diverse, in spite of an identical donor set. The two complexes were also tested for their antimicrobial activity in vitro against seven bacteria and two yeast strains: a minor activity of both complexes vs that of free ligand was given evidence, suggesting both metal ions may possibly play a negative role in vivo.",
publisher = "Amer Chemical Soc, Washington",
journal = "Inorganic Chemistry",
title = "Structural Characterization and Antimicrobial Activity of the Zn(II) Complex with P113 (Demegen), a Derivative of Histatin 5",
volume = "49",
number = "19",
pages = "8690-8698",
doi = "10.1021/ic902547t"
}
Porciatti, E., Milenković, M., Gaggelli, E., Valensin, G., Kozlowski, H., Kamysz, W.,& Valensin, D.. (2010). Structural Characterization and Antimicrobial Activity of the Zn(II) Complex with P113 (Demegen), a Derivative of Histatin 5. in Inorganic Chemistry
Amer Chemical Soc, Washington., 49(19), 8690-8698.
https://doi.org/10.1021/ic902547t
Porciatti E, Milenković M, Gaggelli E, Valensin G, Kozlowski H, Kamysz W, Valensin D. Structural Characterization and Antimicrobial Activity of the Zn(II) Complex with P113 (Demegen), a Derivative of Histatin 5. in Inorganic Chemistry. 2010;49(19):8690-8698.
doi:10.1021/ic902547t .
Porciatti, Elena, Milenković, Marina, Gaggelli, Elena, Valensin, Gianni, Kozlowski, Henryk, Kamysz, Wojciech, Valensin, Daniela, "Structural Characterization and Antimicrobial Activity of the Zn(II) Complex with P113 (Demegen), a Derivative of Histatin 5" in Inorganic Chemistry, 49, no. 19 (2010):8690-8698,
https://doi.org/10.1021/ic902547t . .
