Objectives: In this study we examined the relationship of oxidative stress and hyperglycaemia to antioxidative capacity of high-density cholesterol (HDL-C) particles in type 2 diabetes mellitus (DM). Design and methods: Oxidative stress status parameters (superoxide anion (O-2(-)), superoxide dismutase (SOD) activity and paraoxonase (PON1) status were assessed in 114 patients with type 2 DM and 91 healthy subjects. HDL particle diameters were determined by non-denaturing polyacrylamide gradient (3-31%) gel electrophoresis. Results: Patients had significantly higher concentrations of oxidative stress parameter O-2(-)(p lt 0.001) and antioxidative defence, SOD activity (p lt 0.001). Paraoxonase activity was significantly lower in diabetics (p lt 0.001). The PON1(192) phenotype distribution among study groups was not significantly different. HDL 3 phenotype was significantly prevalent among patients (p lt 0.001). Paraoxonase activity was significantly lower in patients with predominantly ...HDL 2 particles than in controls. Conclusions: The results of our current study indicate that the diabetic HDL 2 phenotype is associated with hyperglycaemia, lower PON1 activity and elevated oxidative stress.
TY - JOUR
AU - Stefanović, Aleksandra
AU - Kotur-Stevuljević, Jelena
AU - Spasić, Slavica
AU - Vekić, Jelena
AU - Zeljković, Aleksandra
AU - Spasojević-Kalimanovska, Vesna
AU - Jelić-Ivanović, Zorana
PY - 2010
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1367
AB - Objectives: In this study we examined the relationship of oxidative stress and hyperglycaemia to antioxidative capacity of high-density cholesterol (HDL-C) particles in type 2 diabetes mellitus (DM). Design and methods: Oxidative stress status parameters (superoxide anion (O-2(-)), superoxide dismutase (SOD) activity and paraoxonase (PON1) status were assessed in 114 patients with type 2 DM and 91 healthy subjects. HDL particle diameters were determined by non-denaturing polyacrylamide gradient (3-31%) gel electrophoresis. Results: Patients had significantly higher concentrations of oxidative stress parameter O-2(-)(p lt 0.001) and antioxidative defence, SOD activity (p lt 0.001). Paraoxonase activity was significantly lower in diabetics (p lt 0.001). The PON1(192) phenotype distribution among study groups was not significantly different. HDL 3 phenotype was significantly prevalent among patients (p lt 0.001). Paraoxonase activity was significantly lower in patients with predominantly HDL 2 particles than in controls. Conclusions: The results of our current study indicate that the diabetic HDL 2 phenotype is associated with hyperglycaemia, lower PON1 activity and elevated oxidative stress.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Clinical Biochemistry
T1 - HDL 2 Particles are associated with hyperglycaemia, lower PON1 activity and oxidative stress in type 2 diabetes mellitus patients
VL - 43
IS - 15
SP - 1230
EP - 1235
DO - 10.1016/j.clinbiochem.2010.08.005
ER -
@article{
author = "Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Spasić, Slavica and Vekić, Jelena and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana",
year = "2010",
abstract = "Objectives: In this study we examined the relationship of oxidative stress and hyperglycaemia to antioxidative capacity of high-density cholesterol (HDL-C) particles in type 2 diabetes mellitus (DM). Design and methods: Oxidative stress status parameters (superoxide anion (O-2(-)), superoxide dismutase (SOD) activity and paraoxonase (PON1) status were assessed in 114 patients with type 2 DM and 91 healthy subjects. HDL particle diameters were determined by non-denaturing polyacrylamide gradient (3-31%) gel electrophoresis. Results: Patients had significantly higher concentrations of oxidative stress parameter O-2(-)(p lt 0.001) and antioxidative defence, SOD activity (p lt 0.001). Paraoxonase activity was significantly lower in diabetics (p lt 0.001). The PON1(192) phenotype distribution among study groups was not significantly different. HDL 3 phenotype was significantly prevalent among patients (p lt 0.001). Paraoxonase activity was significantly lower in patients with predominantly HDL 2 particles than in controls. Conclusions: The results of our current study indicate that the diabetic HDL 2 phenotype is associated with hyperglycaemia, lower PON1 activity and elevated oxidative stress.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "HDL 2 Particles are associated with hyperglycaemia, lower PON1 activity and oxidative stress in type 2 diabetes mellitus patients",
volume = "43",
number = "15",
pages = "1230-1235",
doi = "10.1016/j.clinbiochem.2010.08.005"
}
Stefanović, A., Kotur-Stevuljević, J., Spasić, S., Vekić, J., Zeljković, A., Spasojević-Kalimanovska, V.,& Jelić-Ivanović, Z.. (2010). HDL 2 Particles are associated with hyperglycaemia, lower PON1 activity and oxidative stress in type 2 diabetes mellitus patients. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 43(15), 1230-1235.
https://doi.org/10.1016/j.clinbiochem.2010.08.005
Stefanović A, Kotur-Stevuljević J, Spasić S, Vekić J, Zeljković A, Spasojević-Kalimanovska V, Jelić-Ivanović Z. HDL 2 Particles are associated with hyperglycaemia, lower PON1 activity and oxidative stress in type 2 diabetes mellitus patients. in Clinical Biochemistry. 2010;43(15):1230-1235.
doi:10.1016/j.clinbiochem.2010.08.005 .
Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Spasić, Slavica, Vekić, Jelena, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, "HDL 2 Particles are associated with hyperglycaemia, lower PON1 activity and oxidative stress in type 2 diabetes mellitus patients" in Clinical Biochemistry, 43, no. 15 (2010):1230-1235,
https://doi.org/10.1016/j.clinbiochem.2010.08.005 . .
