An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness.
Source:
Journal of AOAC International, 2010, 93, 1, 102-107
TY - JOUR
AU - Jančić-Stojanović, Biljana
AU - Malenović, Anđelija
AU - Marković, Slavko
AU - Ivanović, Darko
AU - Medenica, Mirjana
PY - 2010
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1371
AB - An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness.
PB - AOAC Int, Gaithersburg
T2 - Journal of AOAC International
T1 - Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories
VL - 93
IS - 1
SP - 102
EP - 107
UR - https://hdl.handle.net/21.15107/rcub_farfar_1371
ER -
@article{
author = "Jančić-Stojanović, Biljana and Malenović, Anđelija and Marković, Slavko and Ivanović, Darko and Medenica, Mirjana",
year = "2010",
abstract = "An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness.",
publisher = "AOAC Int, Gaithersburg",
journal = "Journal of AOAC International",
title = "Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories",
volume = "93",
number = "1",
pages = "102-107",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1371"
}
Jančić-Stojanović, B., Malenović, A., Marković, S., Ivanović, D.,& Medenica, M.. (2010). Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories. in Journal of AOAC International
AOAC Int, Gaithersburg., 93(1), 102-107.
https://hdl.handle.net/21.15107/rcub_farfar_1371
Jančić-Stojanović B, Malenović A, Marković S, Ivanović D, Medenica M. Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories. in Journal of AOAC International. 2010;93(1):102-107.
https://hdl.handle.net/21.15107/rcub_farfar_1371 .
Jančić-Stojanović, Biljana, Malenović, Anđelija, Marković, Slavko, Ivanović, Darko, Medenica, Mirjana, "Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories" in Journal of AOAC International, 93, no. 1 (2010):102-107,
https://hdl.handle.net/21.15107/rcub_farfar_1371 .
