Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories
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Jančić-Stojanović, Biljana
Malenović, Anđelija

Marković, Slavko
Ivanović, Darko
Medenica, Mirjana
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An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness.
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Journal of AOAC International, 2010, 93, 1, 102-107Publisher:
- AOAC Int, Gaithersburg
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PharmacyTY - JOUR AU - Jančić-Stojanović, Biljana AU - Malenović, Anđelija AU - Marković, Slavko AU - Ivanović, Darko AU - Medenica, Mirjana PY - 2010 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1371 AB - An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness. PB - AOAC Int, Gaithersburg T2 - Journal of AOAC International T1 - Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories VL - 93 IS - 1 SP - 102 EP - 107 UR - https://hdl.handle.net/21.15107/rcub_farfar_1371 ER -
@article{ author = "Jančić-Stojanović, Biljana and Malenović, Anđelija and Marković, Slavko and Ivanović, Darko and Medenica, Mirjana", year = "2010", abstract = "An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness.", publisher = "AOAC Int, Gaithersburg", journal = "Journal of AOAC International", title = "Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories", volume = "93", number = "1", pages = "102-107", url = "https://hdl.handle.net/21.15107/rcub_farfar_1371" }
Jančić-Stojanović, B., Malenović, A., Marković, S., Ivanović, D.,& Medenica, M.. (2010). Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories. in Journal of AOAC International AOAC Int, Gaithersburg., 93(1), 102-107. https://hdl.handle.net/21.15107/rcub_farfar_1371
Jančić-Stojanović B, Malenović A, Marković S, Ivanović D, Medenica M. Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories. in Journal of AOAC International. 2010;93(1):102-107. https://hdl.handle.net/21.15107/rcub_farfar_1371 .
Jančić-Stojanović, Biljana, Malenović, Anđelija, Marković, Slavko, Ivanović, Darko, Medenica, Mirjana, "Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories" in Journal of AOAC International, 93, no. 1 (2010):102-107, https://hdl.handle.net/21.15107/rcub_farfar_1371 .