A sensitive and simple spectrophotometric method for the determination of fluorometholone in pharmaceutical formulations using 1,4-dihydrazinophthalazine (1,4-DHPHT; dihydralazine) as the reagent is presented. The yellow coloured dihydralazinohydrazone of fluorometholone was obtained, after heating at 85 degrees C for 2 hrs in acidified 1-propanol as a solvent. The prepared dihydralazinophthalazone shows a maximum at 380 nm with molar absorptivity of 1.91x10(4) lmol(-1)cm(-1). Beers law is obeyed up to 12 mu g/ml of fluorometholone. The method applied for the determination of fluorometholone in pharmaceutical formulations gave precise and reproducible results; recoveries were 97.43% (RSD=2.67%) and 98.25% (RSD=2.56%) for ointment and suppositories, respectively.
TY - JOUR
AU - Vladimirov, Sote
AU - Čudina, Olivera
AU - Agbaba, Danica
AU - Živanov-Stakić, Dobrila
PY - 1996
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/138
AB - A sensitive and simple spectrophotometric method for the determination of fluorometholone in pharmaceutical formulations using 1,4-dihydrazinophthalazine (1,4-DHPHT; dihydralazine) as the reagent is presented. The yellow coloured dihydralazinohydrazone of fluorometholone was obtained, after heating at 85 degrees C for 2 hrs in acidified 1-propanol as a solvent. The prepared dihydralazinophthalazone shows a maximum at 380 nm with molar absorptivity of 1.91x10(4) lmol(-1)cm(-1). Beers law is obeyed up to 12 mu g/ml of fluorometholone. The method applied for the determination of fluorometholone in pharmaceutical formulations gave precise and reproducible results; recoveries were 97.43% (RSD=2.67%) and 98.25% (RSD=2.56%) for ointment and suppositories, respectively.
PB - Marcel Dekker Inc, New York
T2 - Analytical Letters
T1 - Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine
VL - 29
IS - 6
SP - 921
EP - 927
DO - 10.1080/00032719608001444
UR - https://hdl.handle.net/21.15107/rcub_farfar_138
ER -
@article{
author = "Vladimirov, Sote and Čudina, Olivera and Agbaba, Danica and Živanov-Stakić, Dobrila",
year = "1996",
abstract = "A sensitive and simple spectrophotometric method for the determination of fluorometholone in pharmaceutical formulations using 1,4-dihydrazinophthalazine (1,4-DHPHT; dihydralazine) as the reagent is presented. The yellow coloured dihydralazinohydrazone of fluorometholone was obtained, after heating at 85 degrees C for 2 hrs in acidified 1-propanol as a solvent. The prepared dihydralazinophthalazone shows a maximum at 380 nm with molar absorptivity of 1.91x10(4) lmol(-1)cm(-1). Beers law is obeyed up to 12 mu g/ml of fluorometholone. The method applied for the determination of fluorometholone in pharmaceutical formulations gave precise and reproducible results; recoveries were 97.43% (RSD=2.67%) and 98.25% (RSD=2.56%) for ointment and suppositories, respectively.",
publisher = "Marcel Dekker Inc, New York",
journal = "Analytical Letters",
title = "Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine",
volume = "29",
number = "6",
pages = "921-927",
doi = "10.1080/00032719608001444",
url = "https://hdl.handle.net/21.15107/rcub_farfar_138"
}
Vladimirov, S., Čudina, O., Agbaba, D.,& Živanov-Stakić, D.. (1996). Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine. in Analytical Letters
Marcel Dekker Inc, New York., 29(6), 921-927.
https://doi.org/10.1080/00032719608001444
https://hdl.handle.net/21.15107/rcub_farfar_138
Vladimirov S, Čudina O, Agbaba D, Živanov-Stakić D. Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine. in Analytical Letters. 1996;29(6):921-927.
doi:10.1080/00032719608001444
https://hdl.handle.net/21.15107/rcub_farfar_138 .
Vladimirov, Sote, Čudina, Olivera, Agbaba, Danica, Živanov-Stakić, Dobrila, "Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine" in Analytical Letters, 29, no. 6 (1996):921-927,
https://doi.org/10.1080/00032719608001444 .,
https://hdl.handle.net/21.15107/rcub_farfar_138 .
