Stability evaluation of temoporfin-loaded liposomal gels for topical application
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2010
Authors
Dragičević-Curić, NinaWinter, Sven
Krajišnik, Danina

Stupar, Mirjana
Milić, Jela
Graefe, Susanna
Fahr, Alfred
Article (Published version)

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Temoporfin (mTHPC) is a potent second-generation synthetic photosensitizer. Topical delivery of mTHPC is of great interest for the photodynamic therapy of psoriasis and superficial skin cancer lesions. The aim of this study was to evaluate the stability of hydrophilic gels containing mTHPC-loaded liposomes. Two different mTHPC-loaded liposome dispersions, composed of 15 % (w/w) nonhydrogenated soybean lecithin of different phosphatidylcholine content, were prepared and incorporated (2:1 w/w) into hydrogels of different carbomer concentrations (1.5, 2.25, and 3%; w/w). Obtained liposomal hydrogels, containing 0.15% (w/w) mTHPC, 10% (w/w) phospholipids, and 0, 0.5, or 1% (w/w) carbomer, were analyzed for flow properties, liposome particle size, and polydispersity index (PDI), pH value, and mTHPC content after their preparation and at predetermined time intervals during 6 months of storage at 4 and 23 degrees C. All hydrogels showed, during the whole period of investigation, adequate char...acteristics for topical application (i.e., they revealed shear-thinning plastic flow behavior). Rheological parameters, particle size, and PDI of liposomes in hydrogels, mTHPC content, and pH value did not show remarkable changes during the storage of gels, which could make them unacceptable for topical use. The obtained results indicated physical and chemical stability of liposomal gels containing mTHPC during 6 months of storage at both temperatures.
Keywords:
Temoporfin / liposome / liposomal hydrogel / stability / carbomer / rheologySource:
Journal of Liposome Research, 2010, 20, 1, 38-48Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- biolitec AG
- German Academic Exchange Service (DAAD)
DOI: 10.3109/08982100903030263
ISSN: 0898-2104
PubMed: 19558347
WoS: 000274879200006
Scopus: 2-s2.0-77149141801
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Institution/Community
PharmacyTY - JOUR AU - Dragičević-Curić, Nina AU - Winter, Sven AU - Krajišnik, Danina AU - Stupar, Mirjana AU - Milić, Jela AU - Graefe, Susanna AU - Fahr, Alfred PY - 2010 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1386 AB - Temoporfin (mTHPC) is a potent second-generation synthetic photosensitizer. Topical delivery of mTHPC is of great interest for the photodynamic therapy of psoriasis and superficial skin cancer lesions. The aim of this study was to evaluate the stability of hydrophilic gels containing mTHPC-loaded liposomes. Two different mTHPC-loaded liposome dispersions, composed of 15 % (w/w) nonhydrogenated soybean lecithin of different phosphatidylcholine content, were prepared and incorporated (2:1 w/w) into hydrogels of different carbomer concentrations (1.5, 2.25, and 3%; w/w). Obtained liposomal hydrogels, containing 0.15% (w/w) mTHPC, 10% (w/w) phospholipids, and 0, 0.5, or 1% (w/w) carbomer, were analyzed for flow properties, liposome particle size, and polydispersity index (PDI), pH value, and mTHPC content after their preparation and at predetermined time intervals during 6 months of storage at 4 and 23 degrees C. All hydrogels showed, during the whole period of investigation, adequate characteristics for topical application (i.e., they revealed shear-thinning plastic flow behavior). Rheological parameters, particle size, and PDI of liposomes in hydrogels, mTHPC content, and pH value did not show remarkable changes during the storage of gels, which could make them unacceptable for topical use. The obtained results indicated physical and chemical stability of liposomal gels containing mTHPC during 6 months of storage at both temperatures. PB - Taylor & Francis Ltd, Abingdon T2 - Journal of Liposome Research T1 - Stability evaluation of temoporfin-loaded liposomal gels for topical application VL - 20 IS - 1 SP - 38 EP - 48 DO - 10.3109/08982100903030263 ER -
@article{ author = "Dragičević-Curić, Nina and Winter, Sven and Krajišnik, Danina and Stupar, Mirjana and Milić, Jela and Graefe, Susanna and Fahr, Alfred", year = "2010", abstract = "Temoporfin (mTHPC) is a potent second-generation synthetic photosensitizer. Topical delivery of mTHPC is of great interest for the photodynamic therapy of psoriasis and superficial skin cancer lesions. The aim of this study was to evaluate the stability of hydrophilic gels containing mTHPC-loaded liposomes. Two different mTHPC-loaded liposome dispersions, composed of 15 % (w/w) nonhydrogenated soybean lecithin of different phosphatidylcholine content, were prepared and incorporated (2:1 w/w) into hydrogels of different carbomer concentrations (1.5, 2.25, and 3%; w/w). Obtained liposomal hydrogels, containing 0.15% (w/w) mTHPC, 10% (w/w) phospholipids, and 0, 0.5, or 1% (w/w) carbomer, were analyzed for flow properties, liposome particle size, and polydispersity index (PDI), pH value, and mTHPC content after their preparation and at predetermined time intervals during 6 months of storage at 4 and 23 degrees C. All hydrogels showed, during the whole period of investigation, adequate characteristics for topical application (i.e., they revealed shear-thinning plastic flow behavior). Rheological parameters, particle size, and PDI of liposomes in hydrogels, mTHPC content, and pH value did not show remarkable changes during the storage of gels, which could make them unacceptable for topical use. The obtained results indicated physical and chemical stability of liposomal gels containing mTHPC during 6 months of storage at both temperatures.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Journal of Liposome Research", title = "Stability evaluation of temoporfin-loaded liposomal gels for topical application", volume = "20", number = "1", pages = "38-48", doi = "10.3109/08982100903030263" }
Dragičević-Curić, N., Winter, S., Krajišnik, D., Stupar, M., Milić, J., Graefe, S.,& Fahr, A.. (2010). Stability evaluation of temoporfin-loaded liposomal gels for topical application. in Journal of Liposome Research Taylor & Francis Ltd, Abingdon., 20(1), 38-48. https://doi.org/10.3109/08982100903030263
Dragičević-Curić N, Winter S, Krajišnik D, Stupar M, Milić J, Graefe S, Fahr A. Stability evaluation of temoporfin-loaded liposomal gels for topical application. in Journal of Liposome Research. 2010;20(1):38-48. doi:10.3109/08982100903030263 .
Dragičević-Curić, Nina, Winter, Sven, Krajišnik, Danina, Stupar, Mirjana, Milić, Jela, Graefe, Susanna, Fahr, Alfred, "Stability evaluation of temoporfin-loaded liposomal gels for topical application" in Journal of Liposome Research, 20, no. 1 (2010):38-48, https://doi.org/10.3109/08982100903030263 . .