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dc.creatorMarković, Bojan
dc.creatorVladimirov, Sote
dc.creatorČudina, Olivera
dc.creatorSavić, Vladimir
dc.creatorKarljiković-Rajić, Katarina
dc.date.accessioned2019-09-02T11:21:30Z
dc.date.available2019-09-02T11:21:30Z
dc.date.issued2010
dc.identifier.issn1386-1425
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1388
dc.description.abstractA novel topical corticosteroid FA-21-PhP, 2-phenoxypropionate ester of fluocinolone acetonide, has been synthesized in order to investigate the possibility of decreasing systemic side effects. In this study model system for in vitro solvolytic reaction of FA-21-PhP has been analyzed in ethanol/water (90:10, v/v) with excess of sodium hydrogen carbonate. The selected conditions have been used as in vitro model for activation of corticosteroid C-21 ester prodrug. The second-order derivative spectrophotometric method (DS) using zero-crossing technique was developed for monitoring ternary mixture of solvolysis. Fluocinolone acetonide (FA) as a solvolyte was determined in the mixture in the concentration range 0.062-0.312 mM using amplitude (2)D(274.9G). Experimentally determined LOD value was 0.0295 mM. The accuracy of proposed DS method was confirmed with HPLC referent method. Peak area of parent ester FA-21-PhP was used for solvolysis monitoring to ensure the initial stage of changes. Linear relationship in HPLC assay for parent ester was obtained in the concentration range 0.054-0.54 mM, with experimentally determined LOD value of 0.0041 mM. Investigated solvolytic reaction in the presence of excess of NaHCO(3) proceeded via a pseudo-first-order kinetic with significant correlation coefficients 0.9891 and 0.9997 for DS and HPLC, respectively. The values of solvolysis rate constant calculated according to DS and HPLC methods are in good accordance 0.038 and 0.043 h(-1), respectively.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142072/RS//
dc.rightsrestrictedAccess
dc.sourceSpectroscopy Letters
dc.subjectFluocinolone acetonide esteren
dc.subjectDerivative spectrophotometryen
dc.subjectSimultaneous determinationen
dc.subjectSolvolysisen
dc.subjectPseudo-first kinetic orderen
dc.titleAn application of second-order UV-derivative spectrophotometry for study of solvolysis of a novel fluocinolone acetonide esteren
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМарковић, Бојан; Чудина, Оливера; Владимиров, Соте; Савић, Владимир; Карљиковић-Рајић, Катарина;
dc.citation.volume75
dc.citation.issue2
dc.citation.spage930
dc.citation.epage935
dc.citation.other75(2): 930-935
dc.citation.rankM22
dc.identifier.wos000274591200068
dc.identifier.doi10.1016/j.saa.2009.12.043
dc.identifier.pmid20044302
dc.identifier.scopus2-s2.0-73849117515
dc.identifier.rcubconv_2274
dc.type.versionpublishedVersion


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