Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine
Abstract
A sensitive and simple spectrophotometric method for the determination of fluorometholone in pharmaceutical formulations using 1,4-dihydrazinophthalazine (1,4-DHPHT; dihydralazine) as the reagent is presented. The yellow coloured dihydralazinohydrazone of fluorometholone was obtained, after heating at 85 degrees C for 2 hrs in acidified 1-propanol as a solvent. The prepared dihydralazinophthalazone shows a maximum at 380 nm with molar absorptivity of 1.91x10(4) lmol(-1)cm(-1). Beers law is obeyed up to 12 mu g/ml of fluorometholone. The method applied for the determination of fluorometholone in pharmaceutical formulations gave precise and reproducible results; recoveries were 97.43% (RSD=2.67%) and 98.25% (RSD=2.56%) for ointment and suppositories, respectively.
Keywords:
1,4-dihydrazinophthalazine / Fluorometholone / Pharmaceuticals / SpectrophotometrySource:
Analytical Letters, 1996, 29, 6, 921-927Publisher:
- Marcel Dekker Inc, New York
DOI: 10.1080/00032719608001444
ISSN: 0003-2719
WoS: A1996UJ04100006
Scopus: 2-s2.0-2742530210
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Institution/Community
PharmacyTY - JOUR AU - Vladimirov, Sote AU - Čudina, Olivera AU - Agbaba, Danica AU - Živanov-Stakić, Dobrila PY - 1996 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/138 AB - A sensitive and simple spectrophotometric method for the determination of fluorometholone in pharmaceutical formulations using 1,4-dihydrazinophthalazine (1,4-DHPHT; dihydralazine) as the reagent is presented. The yellow coloured dihydralazinohydrazone of fluorometholone was obtained, after heating at 85 degrees C for 2 hrs in acidified 1-propanol as a solvent. The prepared dihydralazinophthalazone shows a maximum at 380 nm with molar absorptivity of 1.91x10(4) lmol(-1)cm(-1). Beers law is obeyed up to 12 mu g/ml of fluorometholone. The method applied for the determination of fluorometholone in pharmaceutical formulations gave precise and reproducible results; recoveries were 97.43% (RSD=2.67%) and 98.25% (RSD=2.56%) for ointment and suppositories, respectively. PB - Marcel Dekker Inc, New York T2 - Analytical Letters T1 - Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine VL - 29 IS - 6 SP - 921 EP - 927 DO - 10.1080/00032719608001444 UR - https://hdl.handle.net/21.15107/rcub_farfar_138 ER -
@article{ author = "Vladimirov, Sote and Čudina, Olivera and Agbaba, Danica and Živanov-Stakić, Dobrila", year = "1996", abstract = "A sensitive and simple spectrophotometric method for the determination of fluorometholone in pharmaceutical formulations using 1,4-dihydrazinophthalazine (1,4-DHPHT; dihydralazine) as the reagent is presented. The yellow coloured dihydralazinohydrazone of fluorometholone was obtained, after heating at 85 degrees C for 2 hrs in acidified 1-propanol as a solvent. The prepared dihydralazinophthalazone shows a maximum at 380 nm with molar absorptivity of 1.91x10(4) lmol(-1)cm(-1). Beers law is obeyed up to 12 mu g/ml of fluorometholone. The method applied for the determination of fluorometholone in pharmaceutical formulations gave precise and reproducible results; recoveries were 97.43% (RSD=2.67%) and 98.25% (RSD=2.56%) for ointment and suppositories, respectively.", publisher = "Marcel Dekker Inc, New York", journal = "Analytical Letters", title = "Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine", volume = "29", number = "6", pages = "921-927", doi = "10.1080/00032719608001444", url = "https://hdl.handle.net/21.15107/rcub_farfar_138" }
Vladimirov, S., Čudina, O., Agbaba, D.,& Živanov-Stakić, D.. (1996). Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine. in Analytical Letters Marcel Dekker Inc, New York., 29(6), 921-927. https://doi.org/10.1080/00032719608001444 https://hdl.handle.net/21.15107/rcub_farfar_138
Vladimirov S, Čudina O, Agbaba D, Živanov-Stakić D. Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine. in Analytical Letters. 1996;29(6):921-927. doi:10.1080/00032719608001444 https://hdl.handle.net/21.15107/rcub_farfar_138 .
Vladimirov, Sote, Čudina, Olivera, Agbaba, Danica, Živanov-Stakić, Dobrila, "Spectrophotometric determination of fluorometholone in pharmaceuticals using 1,4-dihydrazinophthalazine" in Analytical Letters, 29, no. 6 (1996):921-927, https://doi.org/10.1080/00032719608001444 ., https://hdl.handle.net/21.15107/rcub_farfar_138 .