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Role of ovarian hormones in age-associated thymic involution revisited
dc.creator | Perišić, Milica | |
dc.creator | Arsenović-Ranin, Nevena | |
dc.creator | Pilipović, Ivan | |
dc.creator | Kosec, Duško | |
dc.creator | Pešić, Vesna | |
dc.creator | Radojević, Katarina | |
dc.creator | Leposavić, Gordana | |
dc.date.accessioned | 2019-09-02T11:21:52Z | |
dc.date.available | 2019-09-02T11:21:52Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0171-2985 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/1403 | |
dc.description.abstract | A commonly held view that ovarian hormones are causally involved in age-associated thymic involution has been recently challenged. In particular, their relevance in the progression of thymic involution has been disputed. To reassess this issue 10-month-old rats with well advanced thymic involutive changes were ovariectomized (Ovx), and after 1 month thymic cellularity, thymocyte development and levels of recent thymic emigrants (RTEs) were examined in peripheral blood and spleen. In addition, the distribution of major conventional and regulatory T-cell subsets was analyzed in the same peripheral lymphocyte compartments. Ovariectomy increased thymic weight and cellularity above the levels in both 10-month-old and age-matched controls indicating that ovarian hormone ablation not only prevented further progression of thymic involution, hut also reversed it. The increased thymic cellularity was accompanied by altered thymocyte differentiation/maturation culminating in increased thymic output of nave T cells as indicated by elevated levels of both CD4 + and CD8 + RTEs in peripheral blood and spleen. The changes in T-cell development produced: (i) a disproportional increase in cellularity across thymocyte subsets, so that relative proportions of cells at all maturational stages preceding the CD4 + CD8 + T cell receptor (TCR)alpha beta(low) stage were reduced; the relative numbers of CD4 + CD8 + TCR alpha beta(low) cells entering positive selection and their immediate CD4 + CD8 + TCR alpha beta(high) descendents were increased, while those of the most mature CD4 + CD8 and CD4 CD8 + TCR alpha beta(high) cells remained unaltered; (ii) enhanced cell proliferation across all thymocyte subsets and (iii) reduced apoptosis of cells within the CD4 + CD8 + thymocyte subset. The augmented thymic output of naive T cells in Ovx rats most likely reflected an early disinhibition of thymocyte development followed by increased positive/reduced negative selection, at least partly, due to raised thymocyte surface Thy-1 expression. The greater number of CD4 + CD25 + Foxp3 + cells in both thymus and peripheral blood suggested augmented thymic production of these cells. In addition, an increased CD4 + /CD8 + cell ratio was found in the spleen of Ovx rats. Thus, ovarian hormone ablation led not only to increased diversity of the T-cell repertoire, but also to a new balance among distinct T-cell subsets in the periphery. | en |
dc.publisher | Elsevier Gmbh, Urban & Fischer Verlag, Jena | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/145049/RS// | |
dc.rights | restrictedAccess | |
dc.source | Immunobiology | |
dc.subject | Ovarian hormones | en |
dc.subject | Recent thymic emigrants | en |
dc.subject | Thymic involution | en |
dc.subject | Thymopoiesis | en |
dc.subject | T regulatory cells | en |
dc.title | Role of ovarian hormones in age-associated thymic involution revisited | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Aрсеновић-Ранин, Невена; Лепосавић, Гордана; Радојевић, Катарина; Косец, Душко; Пилиповић, Иван; Перишић, Милица; Пешић, Весна; | |
dc.citation.volume | 215 | |
dc.citation.issue | 4 | |
dc.citation.spage | 275 | |
dc.citation.epage | 293 | |
dc.citation.other | 215(4): 275-293 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000276612400003 | |
dc.identifier.doi | 10.1016/j.imbio.2009.06.012 | |
dc.identifier.pmid | 19577818 | |
dc.identifier.scopus | 2-s2.0-77649234731 | |
dc.type.version | publishedVersion |