Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products
Abstract
Multiresponse optimization methodology in combination with experimental design was employed as a powerful technique for simultaneous optimization of input variables significant for evaluation of chromatographic behaviour of zolpidem tartrate, zolpacid, oxozolpidem, zolpyridine and zolpaldehyde towards various responses. In the first stage of the investigation fractional factorial design was used to decrease the number of variables that should be studied in detail. Among examined variables, pH of the mobile phase, percentage of organic modifier and buffer concentration showed to be statistically important and were consequently optimized with central composite design and Derringer's desirability function. Four responses were considered, the retention factors of zolpacid and zolpaldehyde (the first and last peak) and the resolutions between critical peaks. Optimal conditions included Luna C-18(2) analytical column (250 mm x 4.6 mm, 5 mu m particle size), mobile phase consisted of methanol...-10 mM ammonium acetate (68.4:31.6, v/v, pH 5.4) and column temperature of 35 degrees C. The flow rate of the mobile phase was 1 mL min(-1) and the detection was performed at 254 nm. At the end, the method was successfully validated in accordance with ICH guideline and subsequently applied to the analysis of commercially available zolpidem tartrate tablets.
Keywords:
Column liquid chromatography / Fractional factorial design / Central composite design / Derringer's desirability function / Zolpidem tartrateSource:
Chromatographia, 2011, 74, 3-4, 197-208Publisher:
- Springer Heidelberg, Heidelberg
DOI: 10.1007/s10337-011-2064-9
ISSN: 0009-5893
WoS: 000294068100003
Scopus: 2-s2.0-80051547869
Collections
Institution/Community
PharmacyTY - JOUR AU - Malesevic, Marija AU - Živanović, Ljiljana AU - Protić, Ana AU - Jović, Žarko PY - 2011 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1485 AB - Multiresponse optimization methodology in combination with experimental design was employed as a powerful technique for simultaneous optimization of input variables significant for evaluation of chromatographic behaviour of zolpidem tartrate, zolpacid, oxozolpidem, zolpyridine and zolpaldehyde towards various responses. In the first stage of the investigation fractional factorial design was used to decrease the number of variables that should be studied in detail. Among examined variables, pH of the mobile phase, percentage of organic modifier and buffer concentration showed to be statistically important and were consequently optimized with central composite design and Derringer's desirability function. Four responses were considered, the retention factors of zolpacid and zolpaldehyde (the first and last peak) and the resolutions between critical peaks. Optimal conditions included Luna C-18(2) analytical column (250 mm x 4.6 mm, 5 mu m particle size), mobile phase consisted of methanol-10 mM ammonium acetate (68.4:31.6, v/v, pH 5.4) and column temperature of 35 degrees C. The flow rate of the mobile phase was 1 mL min(-1) and the detection was performed at 254 nm. At the end, the method was successfully validated in accordance with ICH guideline and subsequently applied to the analysis of commercially available zolpidem tartrate tablets. PB - Springer Heidelberg, Heidelberg T2 - Chromatographia T1 - Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products VL - 74 IS - 3-4 SP - 197 EP - 208 DO - 10.1007/s10337-011-2064-9 ER -
@article{ author = "Malesevic, Marija and Živanović, Ljiljana and Protić, Ana and Jović, Žarko", year = "2011", abstract = "Multiresponse optimization methodology in combination with experimental design was employed as a powerful technique for simultaneous optimization of input variables significant for evaluation of chromatographic behaviour of zolpidem tartrate, zolpacid, oxozolpidem, zolpyridine and zolpaldehyde towards various responses. In the first stage of the investigation fractional factorial design was used to decrease the number of variables that should be studied in detail. Among examined variables, pH of the mobile phase, percentage of organic modifier and buffer concentration showed to be statistically important and were consequently optimized with central composite design and Derringer's desirability function. Four responses were considered, the retention factors of zolpacid and zolpaldehyde (the first and last peak) and the resolutions between critical peaks. Optimal conditions included Luna C-18(2) analytical column (250 mm x 4.6 mm, 5 mu m particle size), mobile phase consisted of methanol-10 mM ammonium acetate (68.4:31.6, v/v, pH 5.4) and column temperature of 35 degrees C. The flow rate of the mobile phase was 1 mL min(-1) and the detection was performed at 254 nm. At the end, the method was successfully validated in accordance with ICH guideline and subsequently applied to the analysis of commercially available zolpidem tartrate tablets.", publisher = "Springer Heidelberg, Heidelberg", journal = "Chromatographia", title = "Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products", volume = "74", number = "3-4", pages = "197-208", doi = "10.1007/s10337-011-2064-9" }
Malesevic, M., Živanović, L., Protić, A.,& Jović, Ž.. (2011). Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products. in Chromatographia Springer Heidelberg, Heidelberg., 74(3-4), 197-208. https://doi.org/10.1007/s10337-011-2064-9
Malesevic M, Živanović L, Protić A, Jović Ž. Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products. in Chromatographia. 2011;74(3-4):197-208. doi:10.1007/s10337-011-2064-9 .
Malesevic, Marija, Živanović, Ljiljana, Protić, Ana, Jović, Žarko, "Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products" in Chromatographia, 74, no. 3-4 (2011):197-208, https://doi.org/10.1007/s10337-011-2064-9 . .