The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phen...olic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.
TY - JOUR
AU - Zdunić, Gordana
AU - Gođevac, Dejan
AU - Šavikin, Katarina
AU - Novaković, Miroslav
AU - Milosavljević, Slobodan
AU - Petrović, Silvana
PY - 2011
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1542
AB - The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.
PB - Taylor & Francis Ltd, Abingdon
T2 - Natural Product Research
T1 - Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity
VL - 25
IS - 3
SP - 175
EP - 187
DO - 10.1080/14786410802401390
ER -
@article{
author = "Zdunić, Gordana and Gođevac, Dejan and Šavikin, Katarina and Novaković, Miroslav and Milosavljević, Slobodan and Petrović, Silvana",
year = "2011",
abstract = "The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity",
volume = "25",
number = "3",
pages = "175-187",
doi = "10.1080/14786410802401390"
}
Zdunić, G., Gođevac, D., Šavikin, K., Novaković, M., Milosavljević, S.,& Petrović, S.. (2011). Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 25(3), 175-187.
https://doi.org/10.1080/14786410802401390
Zdunić G, Gođevac D, Šavikin K, Novaković M, Milosavljević S, Petrović S. Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research. 2011;25(3):175-187.
doi:10.1080/14786410802401390 .
Zdunić, Gordana, Gođevac, Dejan, Šavikin, Katarina, Novaković, Miroslav, Milosavljević, Slobodan, Petrović, Silvana, "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity" in Natural Product Research, 25, no. 3 (2011):175-187,
https://doi.org/10.1080/14786410802401390 . .
