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Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors
The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed ...by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface.
TY - JOUR
AU - Vovk, Irena
AU - Popović, Gordana
AU - Simonovska, Breda
AU - Albreht, Alen
AU - Agbaba, Danica
PY - 2011
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1570
AB - The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface.
PB - Elsevier Science BV, Amsterdam
T2 - Journal of Chromatography A
T1 - Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors
VL - 1218
IS - 20
SP - 3089
EP - 3094
DO - 10.1016/j.chroma.2011.03.039
UR - conv_2435
ER -
@article{
author = "Vovk, Irena and Popović, Gordana and Simonovska, Breda and Albreht, Alen and Agbaba, Danica",
year = "2011",
abstract = "The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography A",
title = "Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors",
volume = "1218",
number = "20",
pages = "3089-3094",
doi = "10.1016/j.chroma.2011.03.039",
url = "conv_2435"
}
Vovk, I., Popović, G., Simonovska, B., Albreht, A.,& Agbaba, D.. (2011). Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors. in Journal of Chromatography A
Elsevier Science BV, Amsterdam., 1218(20), 3089-3094.
https://doi.org/10.1016/j.chroma.2011.03.039
conv_2435
Vovk I, Popović G, Simonovska B, Albreht A, Agbaba D. Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors. in Journal of Chromatography A. 2011;1218(20):3089-3094.
doi:10.1016/j.chroma.2011.03.039
conv_2435 .
Vovk, Irena, Popović, Gordana, Simonovska, Breda, Albreht, Alen, Agbaba, Danica, "Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors" in Journal of Chromatography A, 1218, no. 20 (2011):3089-3094,
https://doi.org/10.1016/j.chroma.2011.03.039 .,
conv_2435 .
