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Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors

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2011
Authors
Vovk, Irena
Popović, Gordana
Simonovska, Breda
Albreht, Alen
Agbaba, Danica
Article (Published version)
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Abstract
The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed ...by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface.

Keywords:
UTLC / UTLC-MS / TLC / TLC-MS / Densitometry / Image analysis / Prils / Prilates
Source:
Journal of Chromatography A, 2011, 1218, 20, 3089-3094
Publisher:
  • Elsevier Science BV, Amsterdam
Projects:
  • Slovenian Research Agency
  • EN(Right arrow)FIST Centre of Excellence, Slovenia

DOI: 10.1016/j.chroma.2011.03.039

ISSN: 0021-9673

PubMed: 21496825

WoS: 000291065500034

Scopus: 2-s2.0-79955124869
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1570
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Institution
Pharmacy

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