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dc.creatorVovk, Irena
dc.creatorPopović, Gordana
dc.creatorSimonovska, Breda
dc.creatorAlbreht, Alen
dc.creatorAgbaba, Danica
dc.date.accessioned2019-09-02T11:25:42Z
dc.date.available2019-09-02T11:25:42Z
dc.date.issued2011
dc.identifier.issn0021-9673
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/1570
dc.description.abstractThe separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationSlovenian Research Agency
dc.relationEN(Right arrow)FIST Centre of Excellence, Slovenia
dc.rightsrestrictedAccess
dc.sourceJournal of Chromatography A
dc.subjectUTLCen
dc.subjectUTLC-MSen
dc.subjectTLCen
dc.subjectTLC-MSen
dc.subjectDensitometryen
dc.subjectImage analysisen
dc.subjectPrilsen
dc.subjectPrilatesen
dc.titleUltra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitorsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВовк, Ирена; Симоновска, Бреда; Поповић, Гордана; Aлбрехт, Aлен; Aгбаба, Даница;
dc.citation.volume1218
dc.citation.issue20
dc.citation.spage3089
dc.citation.epage3094
dc.citation.other1218(20): 3089-3094
dc.citation.rankaM21
dc.identifier.wos000291065500034
dc.identifier.doi10.1016/j.chroma.2011.03.039
dc.identifier.pmid21496825
dc.identifier.scopus2-s2.0-79955124869
dc.identifier.rcubconv_2435
dc.type.versionpublishedVersion


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