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dc.creatorStojić-Vukanić, Zorica
dc.creatorČolić, Miodrag
dc.creatorBacković, A.
dc.creatorAntić-Stanković, Jelena
dc.creatorBufan, Biljana
dc.creatorDimitrijević, M.
dc.date.accessioned2019-09-02T11:26:01Z
dc.date.available2019-09-02T11:26:01Z
dc.date.issued2011
dc.identifier.issn0354-4664
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1580
dc.description.abstractLeflunomide is an immunosuppressive drug effective in experimental models of transplantation and autoimmune diseases and in the treatment of active rheumatoid arthritis (RA). Having in mind that it has been shown that some other immunosuppressive drugs (glucocorticoids, mycophenolate mofetil, sirolimus etc.) impair dendritic cell (DC) phenotype and function, we investigated the effect of A77 1726, an active metabolite of leflunomide, on the differentiation and function of human monocyte-derived dendritic cells (MDDC) in vitro. Immature MDDC were generated by cultivating monocytes in medium supplemented with GM-CSF and IL-4. To induce maturation, immature MDDC were cultured for 2 additional days with LPS. A77 1726 (100 μM) was added at the beginning of cultivation. Flow cytometric analysis showed that MDDC differentiated in the presence of A77 1726 exhibited an altered phenotype, with a down-regulated surface expression of CD80, CD86, CD54 and CD40 molecules. Furthermore, the continuous presence of A77 1726 during differentiation and maturation prevented successful maturation, judging by the decreased expression of maturation marker CD83, costimulatory and adhesive molecules on A77 1726-treated mature MDDC. In addition, A77 1726-pretreated MDDC exhibited a poor stimulatory capacity of the allogeneic T cells and a low production of IL-10 and IL-18. These data suggest that leflunomide impairs the differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect.en
dc.publisherSrpsko biološko društvo, Beograd, i dr.
dc.rightsopenAccess
dc.sourceArchives of Biological Sciences
dc.subjectAlloreactivityen
dc.subjectcytokine productionen
dc.subjecthuman dendritic cellsen
dc.subjectleflunomideen
dc.subjectphenotypeen
dc.titleDifferentiation and function of human monocyte-derived dendritic cells under the influence of leflunomideen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractЧолић, Миодраг; Бацковић, A.; Димитријевић, М.; Aнтић-Станковић, Јелена; Стојић-Вуканић, Зорица; Буфан, Биљана;
dc.citation.volume63
dc.citation.issue2
dc.citation.spage353
dc.citation.epage364
dc.citation.other63(2): 353-364
dc.citation.rankM23
dc.identifier.wos000291130000008
dc.identifier.doi10.2298/ABS1102353S
dc.identifier.scopus2-s2.0-79961187950
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/428/1578.pdf
dc.identifier.rcubconv_885
dc.type.versionpublishedVersion


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