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Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa

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2012
1627.pdf (592.4Kb)
Authors
Lukić, Jovanka
Strahinić, Ivana
Jovčić, Branko
Filipić, Brankica
Topisirović, Ljubiša
Kojić, Milan
Begović, Jelena
Article (Published version)
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Abstract
Adhesion of bacteria to mucosal surfaces and epithelial cells is one of the key features for the selection of probiotics. In this study, we assessed the adhesion property of Lactococcus lactis subsp. lactis BGKP1 based on its strong autoaggregation phenotype and the presence of the mucin binding protein (MbpL). Genes involved in aggregation (aggL) and possible interaction with mucin (mbpL), present on the same plasmid pKP1, were previously separately cloned in the plasmid pAZIL. In vivo and in vitro experiments revealed potentially different physiological roles of these two proteins in the process of adherence to the intestine during the passage of the strain through the gastrointestinal tract. We correlated the in vitro and in vivo aggregation of the BGKP1-20 carrying plasmid with aggL to binding to the colonic mucus through nonspecific hydrophobic interactions. The expression of AggL on the bacterial cell surface significantly increased the hydrophobicity of the strain. On the other ...hand, the presence of AggL in the strain reduced its ability to adhere to the ileum. Moreover, MbpL protein showed an affinity to bind gastric type mucin proteins such as MUC5AC. This protein did not contribute to the binding of the strain to the ileal or colonic part of the intestine. Different potential functions of lactococcal AggL and MbpL proteins in the process of adhesion to the gastrointestinal tract are proposed.

Source:
Applied and Environmental Microbiology, 2012, 78, 22, 7993-8000
Publisher:
  • Amer Soc Microbiology, Washington
Funding / projects:
  • Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan (RS-173019)

DOI: 10.1128/AEM.02141-12

ISSN: 0099-2240

PubMed: 22961901

WoS: 000310427500022

Scopus: 2-s2.0-84868618717
[ Google Scholar ]
32
29
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/1629
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
AU  - Begović, Jelena
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1629
AB  - Adhesion of bacteria to mucosal surfaces and epithelial cells is one of the key features for the selection of probiotics. In this study, we assessed the adhesion property of Lactococcus lactis subsp. lactis BGKP1 based on its strong autoaggregation phenotype and the presence of the mucin binding protein (MbpL). Genes involved in aggregation (aggL) and possible interaction with mucin (mbpL), present on the same plasmid pKP1, were previously separately cloned in the plasmid pAZIL. In vivo and in vitro experiments revealed potentially different physiological roles of these two proteins in the process of adherence to the intestine during the passage of the strain through the gastrointestinal tract. We correlated the in vitro and in vivo aggregation of the BGKP1-20 carrying plasmid with aggL to binding to the colonic mucus through nonspecific hydrophobic interactions. The expression of AggL on the bacterial cell surface significantly increased the hydrophobicity of the strain. On the other hand, the presence of AggL in the strain reduced its ability to adhere to the ileum. Moreover, MbpL protein showed an affinity to bind gastric type mucin proteins such as MUC5AC. This protein did not contribute to the binding of the strain to the ileal or colonic part of the intestine. Different potential functions of lactococcal AggL and MbpL proteins in the process of adhesion to the gastrointestinal tract are proposed.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa
VL  - 78
IS  - 22
SP  - 7993
EP  - 8000
DO  - 10.1128/AEM.02141-12
ER  - 
@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Jovčić, Branko and Filipić, Brankica and Topisirović, Ljubiša and Kojić, Milan and Begović, Jelena",
year = "2012",
abstract = "Adhesion of bacteria to mucosal surfaces and epithelial cells is one of the key features for the selection of probiotics. In this study, we assessed the adhesion property of Lactococcus lactis subsp. lactis BGKP1 based on its strong autoaggregation phenotype and the presence of the mucin binding protein (MbpL). Genes involved in aggregation (aggL) and possible interaction with mucin (mbpL), present on the same plasmid pKP1, were previously separately cloned in the plasmid pAZIL. In vivo and in vitro experiments revealed potentially different physiological roles of these two proteins in the process of adherence to the intestine during the passage of the strain through the gastrointestinal tract. We correlated the in vitro and in vivo aggregation of the BGKP1-20 carrying plasmid with aggL to binding to the colonic mucus through nonspecific hydrophobic interactions. The expression of AggL on the bacterial cell surface significantly increased the hydrophobicity of the strain. On the other hand, the presence of AggL in the strain reduced its ability to adhere to the ileum. Moreover, MbpL protein showed an affinity to bind gastric type mucin proteins such as MUC5AC. This protein did not contribute to the binding of the strain to the ileal or colonic part of the intestine. Different potential functions of lactococcal AggL and MbpL proteins in the process of adhesion to the gastrointestinal tract are proposed.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa",
volume = "78",
number = "22",
pages = "7993-8000",
doi = "10.1128/AEM.02141-12"
}
Lukić, J., Strahinić, I., Jovčić, B., Filipić, B., Topisirović, L., Kojić, M.,& Begović, J.. (2012). Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 78(22), 7993-8000.
https://doi.org/10.1128/AEM.02141-12
Lukić J, Strahinić I, Jovčić B, Filipić B, Topisirović L, Kojić M, Begović J. Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa. in Applied and Environmental Microbiology. 2012;78(22):7993-8000.
doi:10.1128/AEM.02141-12 .
Lukić, Jovanka, Strahinić, Ivana, Jovčić, Branko, Filipić, Brankica, Topisirović, Ljubiša, Kojić, Milan, Begović, Jelena, "Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa" in Applied and Environmental Microbiology, 78, no. 22 (2012):7993-8000,
https://doi.org/10.1128/AEM.02141-12 . .

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