Background: To investigate the association between clinical and serological features of patients with primary antiphospholipid syndrome (PAPS) and TNF-alpha, interleukin 6 (IL-6), and soluble IL-2 receptor (sIL-2R). Methods: ELISA was used for measurement of antibodies (Abs) and TNF-alpha, while IL-6 and sIL-2R were measured by chemiluminescence. Results: PAPS patients with pulmonary emboli showed positive correlation between IgM isotype of anti-annexin A5 antibodies and TNF-alpha (r = 0.894, p = 0.041) and IgM class of anticardiolipin antibodies and sIL-2R (r = 0.900, p = 0.037). In PAPS with cerebrovascular insults, positive correlation was noticed between TNF-alpha and IgG isotype of anticardiolipin (r = 0.624, p = 0.040) and anti-annexinA5 Abs (r = 0.768, p = 0.006). Conclusions: Isotype analysis of antiphospholipid and anti-annexin A5 Abs and investigation of their association with TNF-alpha is important for differentiation of PAPS patients that are prone to further deterioration ...of arterial and venous thromboses. (Clin. Lab. 2012;58:1079-1084. DOI: 10.7754/Clin.Lab.2012.110918)
TY - JOUR
AU - Becarević, Mirjana
AU - Ignjatović, Svetlana
AU - Majkić-Singh, Nada
PY - 2012
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1632
AB - Background: To investigate the association between clinical and serological features of patients with primary antiphospholipid syndrome (PAPS) and TNF-alpha, interleukin 6 (IL-6), and soluble IL-2 receptor (sIL-2R). Methods: ELISA was used for measurement of antibodies (Abs) and TNF-alpha, while IL-6 and sIL-2R were measured by chemiluminescence. Results: PAPS patients with pulmonary emboli showed positive correlation between IgM isotype of anti-annexin A5 antibodies and TNF-alpha (r = 0.894, p = 0.041) and IgM class of anticardiolipin antibodies and sIL-2R (r = 0.900, p = 0.037). In PAPS with cerebrovascular insults, positive correlation was noticed between TNF-alpha and IgG isotype of anticardiolipin (r = 0.624, p = 0.040) and anti-annexinA5 Abs (r = 0.768, p = 0.006). Conclusions: Isotype analysis of antiphospholipid and anti-annexin A5 Abs and investigation of their association with TNF-alpha is important for differentiation of PAPS patients that are prone to further deterioration of arterial and venous thromboses. (Clin. Lab. 2012;58:1079-1084. DOI: 10.7754/Clin.Lab.2012.110918)
PB - Clin Lab Publ, Heidelberg
T2 - Clinical Laboratory
T1 - Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies
VL - 58
IS - 9-10
SP - 1079
EP - 1084
DO - 10.7754/Clin.Lab.2012.110918
ER -
@article{
author = "Becarević, Mirjana and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2012",
abstract = "Background: To investigate the association between clinical and serological features of patients with primary antiphospholipid syndrome (PAPS) and TNF-alpha, interleukin 6 (IL-6), and soluble IL-2 receptor (sIL-2R). Methods: ELISA was used for measurement of antibodies (Abs) and TNF-alpha, while IL-6 and sIL-2R were measured by chemiluminescence. Results: PAPS patients with pulmonary emboli showed positive correlation between IgM isotype of anti-annexin A5 antibodies and TNF-alpha (r = 0.894, p = 0.041) and IgM class of anticardiolipin antibodies and sIL-2R (r = 0.900, p = 0.037). In PAPS with cerebrovascular insults, positive correlation was noticed between TNF-alpha and IgG isotype of anticardiolipin (r = 0.624, p = 0.040) and anti-annexinA5 Abs (r = 0.768, p = 0.006). Conclusions: Isotype analysis of antiphospholipid and anti-annexin A5 Abs and investigation of their association with TNF-alpha is important for differentiation of PAPS patients that are prone to further deterioration of arterial and venous thromboses. (Clin. Lab. 2012;58:1079-1084. DOI: 10.7754/Clin.Lab.2012.110918)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies",
volume = "58",
number = "9-10",
pages = "1079-1084",
doi = "10.7754/Clin.Lab.2012.110918"
}
Becarević, M., Ignjatović, S.,& Majkić-Singh, N.. (2012). Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 58(9-10), 1079-1084.
https://doi.org/10.7754/Clin.Lab.2012.110918
Becarević M, Ignjatović S, Majkić-Singh N. Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies. in Clinical Laboratory. 2012;58(9-10):1079-1084.
doi:10.7754/Clin.Lab.2012.110918 .
Becarević, Mirjana, Ignjatović, Svetlana, Majkić-Singh, Nada, "Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies" in Clinical Laboratory, 58, no. 9-10 (2012):1079-1084,
https://doi.org/10.7754/Clin.Lab.2012.110918 . .
