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dc.creatorRakić, Tijana
dc.creatorJančić-Stojanović, Biljana
dc.creatorMalenović, Anđelija
dc.creatorIvanović, Darko
dc.creatorMedenica, Mirjana
dc.date.accessioned2019-09-02T11:27:35Z
dc.date.available2019-09-02T11:27:35Z
dc.date.issued2012
dc.identifier.issn0039-9140
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1645
dc.description.abstractThis paper presents exploration of chromatographic behavior in HILIC system by experimental design and improved chromatographic response function denoted as N-CRF*. As a model mixture six antidepressants were chosen: selegiline, mianserine, sertraline, moclobemide, fluoxetine and maprotiline. Due to complexity of retention mechanisms in HILIC system, detailed examination of experimental space assessing the influence of important factors (acetonitrile content in the mobile phase, buffer concentration and pH of the mobile phase) and their interactions was done by applying 3(3) experimental design. N-CRF*. is developed and designed to be the only output of the system which simultaneously measures the separation of all the examined substances, the chromatographic run duration and the quality of the obtained peaks shape. It allowed objective estimation of overall chromatogram quality and excluded the arbitrary judgment in ambiguous situations. The applied function highlighted the influence of investigated factors on entire mixture and enabled identification of experimental regions where the chromatographic behavior was satisfactory. Applied experimental design strategy combined with N-CRF* proved to be valuable assistance in HILIC separation of complex mixtures.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceTalanta
dc.subjectHydrophilic interaction liquiden
dc.subjectchromatography (HILIC)en
dc.subjectChromatographic response function (N-CRF*)en
dc.subject3(3) Experimental designen
dc.subjectAntidepressantsen
dc.titleImproved chromatographic response function in HILIC analysis: Application to mixture of antidepressantsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаленовић, Aнђелија; Ракић, Тијана; Ивановић, Дарко; Јанчић-Стојановић, Биљана; Меденица, Мирјана;
dc.citation.volume98
dc.citation.spage54
dc.citation.epage61
dc.citation.other98: 54-61
dc.citation.rankM21
dc.identifier.wos000309327600009
dc.identifier.doi10.1016/j.talanta.2012.06.040
dc.identifier.pmid22939128
dc.identifier.scopus2-s2.0-84865689490
dc.identifier.rcubconv_2698
dc.type.versionpublishedVersion


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