Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique
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Simple and reliable novel methods for the determination of uric acid (UA) are proposed and validated. For quantitative determination of UA, two matrices were used: the Bray-Liebhafsky (BL) oscillatory reaction in a stable non-equilibrium stationary state close to the bifurcation point (method A) as well as, the BL non-oscillating subsystem (mixture KIO3 and H2SO4), i.e., Dushman reaction (DR) in a steady state (method B). The proposed methods are optimized in a continuously fed well stirred tank reactor (CSTR) and applied with excellent results in the determination of UA in human urine samples. The linear relationship between maximal potential shift Delta E-m, and both the logarithm of the UA concentration (procedure A) and UA concentration (procedure B) is obtained in the concentration range 2.98 x 10(-5)-2.68 x 10(-4) mol L-1 and 2.98 x 10(-5)-3.58 x 10(-4) mol L-1, respectively. The methods have an excellent sample throughput of 30 samples h(-1) (method A) and 7 samples h(-1) (metho...d B) with the sensitivity determined to be 1.1 x 10(-5) mol L-1 (method A) and 8.9 x 10(-6) mol L-1 (method B) as well as the precision RSD lt = 3.4% for both methods. Some aspects of the possible mechanism of UA action on the BL oscillating and Duschman non-oscillating reaction systems are discussed in detail.
Keywords:uric acid / perturbation technique / Bray-Liebhafsky oscillatory reaction / Dushman reaction / urine
Source:Journal of the Brazilian Chemical Society, 2012, 23, 8, 1450-1459
- Soc Brasileira Quimica, Sao Paulo