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dc.creatorPejić, Nataša
dc.creatorMaksimović, Jelena
dc.creatorBlagojević, Slavica
dc.creatorAnić, Slobodan
dc.creatorČupić, Željko
dc.creatorKolar-Anić, Ljiljana
dc.date.accessioned2019-09-02T11:27:50Z
dc.date.available2019-09-02T11:27:50Z
dc.date.issued2012
dc.identifier.issn0103-5053
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1655
dc.description.abstractSimple and reliable novel methods for the determination of uric acid (UA) are proposed and validated. For quantitative determination of UA, two matrices were used: the Bray-Liebhafsky (BL) oscillatory reaction in a stable non-equilibrium stationary state close to the bifurcation point (method A) as well as, the BL non-oscillating subsystem (mixture KIO3 and H2SO4), i.e., Dushman reaction (DR) in a steady state (method B). The proposed methods are optimized in a continuously fed well stirred tank reactor (CSTR) and applied with excellent results in the determination of UA in human urine samples. The linear relationship between maximal potential shift Delta E-m, and both the logarithm of the UA concentration (procedure A) and UA concentration (procedure B) is obtained in the concentration range 2.98 x 10(-5)-2.68 x 10(-4) mol L-1 and 2.98 x 10(-5)-3.58 x 10(-4) mol L-1, respectively. The methods have an excellent sample throughput of 30 samples h(-1) (method A) and 7 samples h(-1) (method B) with the sensitivity determined to be 1.1 x 10(-5) mol L-1 (method A) and 8.9 x 10(-6) mol L-1 (method B) as well as the precision RSD lt = 3.4% for both methods. Some aspects of the possible mechanism of UA action on the BL oscillating and Duschman non-oscillating reaction systems are discussed in detail.en
dc.publisherSoc Brasileira Quimica, Sao Paulo
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172015/RS//
dc.rightsopenAccess
dc.sourceJournal of the Brazilian Chemical Society
dc.subjecturic aciden
dc.subjectperturbation techniqueen
dc.subjectBray-Liebhafsky oscillatory reactionen
dc.subjectDushman reactionen
dc.subjecturineen
dc.titleKinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Techniqueen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractМаксимовић, Јелена; Колар-Aнић, Љиљана; Благојевић, Славица; Aнић, Слободан; Чупић, Жељко; Пејић, Наташа;
dc.citation.volume23
dc.citation.issue8
dc.citation.spage1450
dc.citation.epage1459
dc.citation.other23(8): 1450-1459
dc.citation.rankM22
dc.identifier.wos000309162200007
dc.identifier.scopus2-s2.0-84866410166
dc.identifier.rcubconv_2693
dc.type.versionpublishedVersion


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