dc.creator | Đukić, Mirjana | |
dc.creator | Jovanović, Marina | |
dc.creator | Ninković, Milica | |
dc.creator | Stevanović, Ivana | |
dc.creator | Ćurčić, Marijana | |
dc.creator | Topić, Aleksandra | |
dc.creator | Vujanović, Dragana | |
dc.creator | Đurđević, Dragan | |
dc.date.accessioned | 2019-09-02T11:28:03Z | |
dc.date.available | 2019-09-02T11:28:03Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 1232-1966 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/1663 | |
dc.description.abstract | Introduction: Contact herbicide diquat (DQ), redox cycling compound, mediates its systemic toxicity throughout the enlarged production of free radicals. Target organs are liver and kidney in humans. To-date, the mechanism of DQ-induced neurotoxicity has not been rationalized. Objective: The objectives of the study were to examine the ability of DQ to induce oxidative stress (OS) and/or nitrosative stress (NS) upon intrastriatal (i.s.) administration and to investigate the role of nitric oxide (NOx) using NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of DQ i.s. administration. Material and Methods: The experiment was conducted on Wistar rats, randomly divided in experimental groups, receiving different treatments i.s. applied. Parameters of OS/NS such as: superoxide anion radical (O-2(center dot-)), superoxide dismutase (SOD), malondialdehyde (MDA) and nitrates (NO3-) were measured in the cortex (bilaterally), at 30th min, 24 hours and 7 days after the treatments. Results: Lethargy and high mortality rate were observed only in the DQ group (within 24 hours and 2-3 hours, respectively) after awakening from anesthesia. Markedly increased production of NOx and O-2(center dot-) along with elevated lipid peroxidation altogether contributed to DQ neurotoxicity. The most importantly, the L-NAME i.s. pretreatment protected treated animals from dying and diminished OS/NS response against DQ-induced neurotoxicity. Conclusion: The i.s. pretreatment with L-NAME resulted in neuroprotection against DQ neurotoxity, based on animal survival and reduced LPO in the cortex. | en |
dc.publisher | Inst Agricultural Medicine, Lublin | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41018/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173008/RS// | |
dc.relation | Ministry of Defense of the Republic of Serbia (Project No.
MMA/06-10/B.3). | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.source | Annals of Agricultural and Environmental Medicine | |
dc.subject | diquat | en |
dc.subject | oxidative stress | en |
dc.subject | nitric oxide | en |
dc.subject | L-NAME | en |
dc.subject | Wistar rats | en |
dc.subject | brain | en |
dc.title | Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity | en |
dc.type | article | |
dc.rights.license | BY-NC | |
dcterms.abstract | Топић, Aлександра; Ђурђевић, Драган; Нинковић, Милица; Вујановић, Драгана; Јовановић, Марина; Ђукић, Мирјана; Ћурчић, Маријана; Стевановић, Ивана; | |
dc.citation.volume | 19 | |
dc.citation.issue | 4 | |
dc.citation.spage | 666 | |
dc.citation.epage | 672 | |
dc.citation.other | 19(4): 666-672 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000313298600011 | |
dc.identifier.pmid | 23311786 | |
dc.identifier.scopus | 2-s2.0-84871589433 | |
dc.identifier.fulltext | http://farfar.pharmacy.bg.ac.rs/bitstream/id/11869/Intrastriatal_pre_treatment_pub_2012.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_farfar_1663 | |
dc.type.version | publishedVersion | |