High performance liquid chromatographic determination of clindamycin in pharmaceutical formulations
Apstrakt
A specific and simple HPLC procedure was developed, su table for the determination of dindamycin hydrochloride monohydrate (CLHC) and clindamycin 2-dihydrogenphosphate (CLPH) in pharmaceutical formulations. Rp-HPLC analysis was performed with a LCD-Analytical Consta Metric 3000 system, equipped with a Spectro Monitor 3100 UV/VIS detector. Lincosamides assay procedures were carried out on a Supelcosil R LC-18-DB (250 × 4,6 mm, 5 μm particles) column in an acetonitrile-potassium dihydrogenphosphate buffer (0.1 M; pH 2.50) (225:775 v/v) as mobile phase. Column effluents were monitored at 206 nm for clindamycin. The calibration curves in concentration ranges from 200 to 1800 μg/ml were established for the investigated clindamycins. The HPLC method was applied for the determination of clindamycin in pharmaceutical formulations. The obtained results and statistical parameters for both the investigated clindamycins in pharmaceutical formulations, were satisfactory and ranged from 99.99% to 10...4.18% (R.S.D. was ranged from 0.79% to 0.99%).
Ključne reči:
Clindamycin / HPLC determination / Pharmaceutical formulationsIzvor:
Journal of the Serbian Chemical Society, 1997, 62, 2, 177-181Izdavač:
- Srpsko hemijsko društvo, Beograd
Institucija/grupa
PharmacyTY - JOUR AU - Vladimirov, S AU - Marković, L AU - Agbaba, Danica AU - Živanov-Stakić, Dobrila AU - Brborić, Jasmina PY - 1997 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/166 AB - A specific and simple HPLC procedure was developed, su table for the determination of dindamycin hydrochloride monohydrate (CLHC) and clindamycin 2-dihydrogenphosphate (CLPH) in pharmaceutical formulations. Rp-HPLC analysis was performed with a LCD-Analytical Consta Metric 3000 system, equipped with a Spectro Monitor 3100 UV/VIS detector. Lincosamides assay procedures were carried out on a Supelcosil R LC-18-DB (250 × 4,6 mm, 5 μm particles) column in an acetonitrile-potassium dihydrogenphosphate buffer (0.1 M; pH 2.50) (225:775 v/v) as mobile phase. Column effluents were monitored at 206 nm for clindamycin. The calibration curves in concentration ranges from 200 to 1800 μg/ml were established for the investigated clindamycins. The HPLC method was applied for the determination of clindamycin in pharmaceutical formulations. The obtained results and statistical parameters for both the investigated clindamycins in pharmaceutical formulations, were satisfactory and ranged from 99.99% to 104.18% (R.S.D. was ranged from 0.79% to 0.99%). PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - High performance liquid chromatographic determination of clindamycin in pharmaceutical formulations VL - 62 IS - 2 SP - 177 EP - 181 UR - https://hdl.handle.net/21.15107/rcub_farfar_166 ER -
@article{ author = "Vladimirov, S and Marković, L and Agbaba, Danica and Živanov-Stakić, Dobrila and Brborić, Jasmina", year = "1997", abstract = "A specific and simple HPLC procedure was developed, su table for the determination of dindamycin hydrochloride monohydrate (CLHC) and clindamycin 2-dihydrogenphosphate (CLPH) in pharmaceutical formulations. Rp-HPLC analysis was performed with a LCD-Analytical Consta Metric 3000 system, equipped with a Spectro Monitor 3100 UV/VIS detector. Lincosamides assay procedures were carried out on a Supelcosil R LC-18-DB (250 × 4,6 mm, 5 μm particles) column in an acetonitrile-potassium dihydrogenphosphate buffer (0.1 M; pH 2.50) (225:775 v/v) as mobile phase. Column effluents were monitored at 206 nm for clindamycin. The calibration curves in concentration ranges from 200 to 1800 μg/ml were established for the investigated clindamycins. The HPLC method was applied for the determination of clindamycin in pharmaceutical formulations. The obtained results and statistical parameters for both the investigated clindamycins in pharmaceutical formulations, were satisfactory and ranged from 99.99% to 104.18% (R.S.D. was ranged from 0.79% to 0.99%).", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "High performance liquid chromatographic determination of clindamycin in pharmaceutical formulations", volume = "62", number = "2", pages = "177-181", url = "https://hdl.handle.net/21.15107/rcub_farfar_166" }
Vladimirov, S., Marković, L., Agbaba, D., Živanov-Stakić, D.,& Brborić, J.. (1997). High performance liquid chromatographic determination of clindamycin in pharmaceutical formulations. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 62(2), 177-181. https://hdl.handle.net/21.15107/rcub_farfar_166
Vladimirov S, Marković L, Agbaba D, Živanov-Stakić D, Brborić J. High performance liquid chromatographic determination of clindamycin in pharmaceutical formulations. in Journal of the Serbian Chemical Society. 1997;62(2):177-181. https://hdl.handle.net/21.15107/rcub_farfar_166 .
Vladimirov, S, Marković, L, Agbaba, Danica, Živanov-Stakić, Dobrila, Brborić, Jasmina, "High performance liquid chromatographic determination of clindamycin in pharmaceutical formulations" in Journal of the Serbian Chemical Society, 62, no. 2 (1997):177-181, https://hdl.handle.net/21.15107/rcub_farfar_166 .