Role of TRPC3 Channel in Human Internal Mammary Artery
Authorized Users Only
2012
Authors
Gao, GeBai, Xiao-Yan
Xuan, Chao
Liu, Xiao-Cheng
Jing, Wen-Bin
Novaković, Aleksandra

Yang, Qin

He, Guo-Wei
Article (Published version)

Metadata
Show full item recordAbstract
Background and Aims. Intracellular calcium regulation in endothelial cells depends on transient receptor potential channels (TRPs). Canonical TRPs (TRPCs) are now recognized as the most important Ca2+-permeable cation channels in vascular endothelium and TRPC3 channel is reported to play a role in vasodilation in animal vessels. However, little is known about the role of TRPCs in human arteries. We therefore tested the hypothesis that TRPCs play a role in human arteries. Methods. Cumulative concentration-relaxation curves to acetylcholine (-11 to -4.5 log M) were established in the human internal mammary artery (IMA) rings (n = 42) taken from 28 patients undergoing coronary artery bypass grafting in precontraction induced by U46619 (-8 log M) in the absence or presence of SKF96365 (10 mu mol/L) or Pyr3 (3 mu mol/L). Protein expressions of TRPC3 were determined by Western blot and immunohistochemistry staining. Results. The maximal relaxation induced by acetylcholine was significantly a...ttenuated by the nonspecific cation channels inhibitor, SKF96365 (48.2 +/- 3.7 vs. 66.0 +/- 0.9% in control, p lt 0.01) or the selective TRPC3 blocker, Pyr3 (58.4 +/- 2.3% vs. 67.7 +/- 1.1% in control, p lt 0.01). Protein expression of TRPC3 was detected in human 1MA. Conclusions. TRPC3 exists and plays a role in the acetylcholine-induced endothelium-dependent relaxation in the human IMA. This study suggests that TRPC3 may be a potential new target in endothelial protection in patients with endothelial dysfunction such as in patients with coronary artery disease in order to improve the long-term patency of the grafting vessels.
Keywords:
TRPC3 / Ion channel / Vasorelaxation / VasospasmSource:
Archives of Medical Research, 2012, 43, 6, 431-437Publisher:
- Elsevier Science Inc, New York
Funding / projects:
- International S & T Cooperation Program of China - 2009DFB30560
DOI: 10.1016/j.arcmed.2012.08.010
ISSN: 0188-4409
PubMed: 22960861
WoS: 000311766200003
Scopus: 2-s2.0-84867849716
Collections
Institution/Community
PharmacyTY - JOUR AU - Gao, Ge AU - Bai, Xiao-Yan AU - Xuan, Chao AU - Liu, Xiao-Cheng AU - Jing, Wen-Bin AU - Novaković, Aleksandra AU - Yang, Qin AU - He, Guo-Wei PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1680 AB - Background and Aims. Intracellular calcium regulation in endothelial cells depends on transient receptor potential channels (TRPs). Canonical TRPs (TRPCs) are now recognized as the most important Ca2+-permeable cation channels in vascular endothelium and TRPC3 channel is reported to play a role in vasodilation in animal vessels. However, little is known about the role of TRPCs in human arteries. We therefore tested the hypothesis that TRPCs play a role in human arteries. Methods. Cumulative concentration-relaxation curves to acetylcholine (-11 to -4.5 log M) were established in the human internal mammary artery (IMA) rings (n = 42) taken from 28 patients undergoing coronary artery bypass grafting in precontraction induced by U46619 (-8 log M) in the absence or presence of SKF96365 (10 mu mol/L) or Pyr3 (3 mu mol/L). Protein expressions of TRPC3 were determined by Western blot and immunohistochemistry staining. Results. The maximal relaxation induced by acetylcholine was significantly attenuated by the nonspecific cation channels inhibitor, SKF96365 (48.2 +/- 3.7 vs. 66.0 +/- 0.9% in control, p lt 0.01) or the selective TRPC3 blocker, Pyr3 (58.4 +/- 2.3% vs. 67.7 +/- 1.1% in control, p lt 0.01). Protein expression of TRPC3 was detected in human 1MA. Conclusions. TRPC3 exists and plays a role in the acetylcholine-induced endothelium-dependent relaxation in the human IMA. This study suggests that TRPC3 may be a potential new target in endothelial protection in patients with endothelial dysfunction such as in patients with coronary artery disease in order to improve the long-term patency of the grafting vessels. PB - Elsevier Science Inc, New York T2 - Archives of Medical Research T1 - Role of TRPC3 Channel in Human Internal Mammary Artery VL - 43 IS - 6 SP - 431 EP - 437 DO - 10.1016/j.arcmed.2012.08.010 ER -
@article{ author = "Gao, Ge and Bai, Xiao-Yan and Xuan, Chao and Liu, Xiao-Cheng and Jing, Wen-Bin and Novaković, Aleksandra and Yang, Qin and He, Guo-Wei", year = "2012", abstract = "Background and Aims. Intracellular calcium regulation in endothelial cells depends on transient receptor potential channels (TRPs). Canonical TRPs (TRPCs) are now recognized as the most important Ca2+-permeable cation channels in vascular endothelium and TRPC3 channel is reported to play a role in vasodilation in animal vessels. However, little is known about the role of TRPCs in human arteries. We therefore tested the hypothesis that TRPCs play a role in human arteries. Methods. Cumulative concentration-relaxation curves to acetylcholine (-11 to -4.5 log M) were established in the human internal mammary artery (IMA) rings (n = 42) taken from 28 patients undergoing coronary artery bypass grafting in precontraction induced by U46619 (-8 log M) in the absence or presence of SKF96365 (10 mu mol/L) or Pyr3 (3 mu mol/L). Protein expressions of TRPC3 were determined by Western blot and immunohistochemistry staining. Results. The maximal relaxation induced by acetylcholine was significantly attenuated by the nonspecific cation channels inhibitor, SKF96365 (48.2 +/- 3.7 vs. 66.0 +/- 0.9% in control, p lt 0.01) or the selective TRPC3 blocker, Pyr3 (58.4 +/- 2.3% vs. 67.7 +/- 1.1% in control, p lt 0.01). Protein expression of TRPC3 was detected in human 1MA. Conclusions. TRPC3 exists and plays a role in the acetylcholine-induced endothelium-dependent relaxation in the human IMA. This study suggests that TRPC3 may be a potential new target in endothelial protection in patients with endothelial dysfunction such as in patients with coronary artery disease in order to improve the long-term patency of the grafting vessels.", publisher = "Elsevier Science Inc, New York", journal = "Archives of Medical Research", title = "Role of TRPC3 Channel in Human Internal Mammary Artery", volume = "43", number = "6", pages = "431-437", doi = "10.1016/j.arcmed.2012.08.010" }
Gao, G., Bai, X., Xuan, C., Liu, X., Jing, W., Novaković, A., Yang, Q.,& He, G.. (2012). Role of TRPC3 Channel in Human Internal Mammary Artery. in Archives of Medical Research Elsevier Science Inc, New York., 43(6), 431-437. https://doi.org/10.1016/j.arcmed.2012.08.010
Gao G, Bai X, Xuan C, Liu X, Jing W, Novaković A, Yang Q, He G. Role of TRPC3 Channel in Human Internal Mammary Artery. in Archives of Medical Research. 2012;43(6):431-437. doi:10.1016/j.arcmed.2012.08.010 .
Gao, Ge, Bai, Xiao-Yan, Xuan, Chao, Liu, Xiao-Cheng, Jing, Wen-Bin, Novaković, Aleksandra, Yang, Qin, He, Guo-Wei, "Role of TRPC3 Channel in Human Internal Mammary Artery" in Archives of Medical Research, 43, no. 6 (2012):431-437, https://doi.org/10.1016/j.arcmed.2012.08.010 . .