Physicochemical Factors Governing the Partition of Pramipexole and Its Five Impurities in Microemulsion Liquid Chromatographic Systems
2012
Authors
Vemić, AnaMalenović, Anđelija
Rakić, Tijana
Kostić, Nada M.
Jančić-Stojanović, Biljana
Ivanović, Darko
Medenica, Mirjana
Article (Published version)
Metadata
Show full item recordAbstract
Microemulsions when used as mobile phases represent a very powerful chromatographic tool due to their polyphase structure. The aim of this study was to investigate the influence of the mobile phase composition and resulting microemulsion droplet's properties, as well as the stationary phase lipophilicity on the chromatographic behavior of pramipexole and its five impurities. Investigation was carried out according to the experimental plan defined by a full factorial design 2(4) with four central point replications. While the most affected by column characteristics was the last eluting, most lipophilic substance, the composition and characteristics of microemulsion droplets were equally important, so the partition system microemulsion droplets/stationary phase proved to be the most significant. Stationary phase lipophilicity did not affect the most hydrophilic component and only the partition system microemulsion droplets/bulk of the eluent impacted its behavior. For the separation of t...he critical pair, almost equal effects of the microemulsion droplets/stationary phase and bulk of the eluent/stationary phase partition systems were noted.
Keywords:
microemulsion liquid chromatography / MELC / partition behavior / experimental design / column lipophilicity / pramipexoleSource:
Journal of the Brazilian Chemical Society, 2012, 23, 11, 2084-2092Publisher:
- Soc Brasileira Quimica, Sao Paulo
Funding / projects:
- Modelling of different chromatographic systems with chemometrical approach in pharmaceutical analysis (RS-MESTD-Basic Research (BR or ON)-172052)
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Institution/Community
PharmacyTY - JOUR AU - Vemić, Ana AU - Malenović, Anđelija AU - Rakić, Tijana AU - Kostić, Nada M. AU - Jančić-Stojanović, Biljana AU - Ivanović, Darko AU - Medenica, Mirjana PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1681 AB - Microemulsions when used as mobile phases represent a very powerful chromatographic tool due to their polyphase structure. The aim of this study was to investigate the influence of the mobile phase composition and resulting microemulsion droplet's properties, as well as the stationary phase lipophilicity on the chromatographic behavior of pramipexole and its five impurities. Investigation was carried out according to the experimental plan defined by a full factorial design 2(4) with four central point replications. While the most affected by column characteristics was the last eluting, most lipophilic substance, the composition and characteristics of microemulsion droplets were equally important, so the partition system microemulsion droplets/stationary phase proved to be the most significant. Stationary phase lipophilicity did not affect the most hydrophilic component and only the partition system microemulsion droplets/bulk of the eluent impacted its behavior. For the separation of the critical pair, almost equal effects of the microemulsion droplets/stationary phase and bulk of the eluent/stationary phase partition systems were noted. PB - Soc Brasileira Quimica, Sao Paulo T2 - Journal of the Brazilian Chemical Society T1 - Physicochemical Factors Governing the Partition of Pramipexole and Its Five Impurities in Microemulsion Liquid Chromatographic Systems VL - 23 IS - 11 SP - 2084 EP - 2092 UR - https://hdl.handle.net/21.15107/rcub_farfar_1681 ER -
@article{ author = "Vemić, Ana and Malenović, Anđelija and Rakić, Tijana and Kostić, Nada M. and Jančić-Stojanović, Biljana and Ivanović, Darko and Medenica, Mirjana", year = "2012", abstract = "Microemulsions when used as mobile phases represent a very powerful chromatographic tool due to their polyphase structure. The aim of this study was to investigate the influence of the mobile phase composition and resulting microemulsion droplet's properties, as well as the stationary phase lipophilicity on the chromatographic behavior of pramipexole and its five impurities. Investigation was carried out according to the experimental plan defined by a full factorial design 2(4) with four central point replications. While the most affected by column characteristics was the last eluting, most lipophilic substance, the composition and characteristics of microemulsion droplets were equally important, so the partition system microemulsion droplets/stationary phase proved to be the most significant. Stationary phase lipophilicity did not affect the most hydrophilic component and only the partition system microemulsion droplets/bulk of the eluent impacted its behavior. For the separation of the critical pair, almost equal effects of the microemulsion droplets/stationary phase and bulk of the eluent/stationary phase partition systems were noted.", publisher = "Soc Brasileira Quimica, Sao Paulo", journal = "Journal of the Brazilian Chemical Society", title = "Physicochemical Factors Governing the Partition of Pramipexole and Its Five Impurities in Microemulsion Liquid Chromatographic Systems", volume = "23", number = "11", pages = "2084-2092", url = "https://hdl.handle.net/21.15107/rcub_farfar_1681" }
Vemić, A., Malenović, A., Rakić, T., Kostić, N. M., Jančić-Stojanović, B., Ivanović, D.,& Medenica, M.. (2012). Physicochemical Factors Governing the Partition of Pramipexole and Its Five Impurities in Microemulsion Liquid Chromatographic Systems. in Journal of the Brazilian Chemical Society Soc Brasileira Quimica, Sao Paulo., 23(11), 2084-2092. https://hdl.handle.net/21.15107/rcub_farfar_1681
Vemić A, Malenović A, Rakić T, Kostić NM, Jančić-Stojanović B, Ivanović D, Medenica M. Physicochemical Factors Governing the Partition of Pramipexole and Its Five Impurities in Microemulsion Liquid Chromatographic Systems. in Journal of the Brazilian Chemical Society. 2012;23(11):2084-2092. https://hdl.handle.net/21.15107/rcub_farfar_1681 .
Vemić, Ana, Malenović, Anđelija, Rakić, Tijana, Kostić, Nada M., Jančić-Stojanović, Biljana, Ivanović, Darko, Medenica, Mirjana, "Physicochemical Factors Governing the Partition of Pramipexole and Its Five Impurities in Microemulsion Liquid Chromatographic Systems" in Journal of the Brazilian Chemical Society, 23, no. 11 (2012):2084-2092, https://hdl.handle.net/21.15107/rcub_farfar_1681 .