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dc.creatorJovović, M.
dc.creatorKostić, Nada
dc.creatorJančić-Stojanović, Biljana
dc.creatorMalenović, Anđelija
dc.date.accessioned2019-09-02T11:28:49Z
dc.date.available2019-09-02T11:28:49Z
dc.date.issued2012
dc.identifier.issn1082-6076
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1696
dc.description.abstractIn this study, a development of stability indicating method (SIM) that could be used to detect a decrease in the amount of tropicamide and benzalkonium chloride due to their degradation is described. In the method development, stress samples should be used. Benzalkanium chloride proved to be very stable under all stress conditions. Acid stress with 0.1 M HCl C led to 10% degradation of tropicamide alter >= 30 min. The alkaline stress with 1 M NaOH at 70 degrees C induced faster degradation and more than 20% of tropicamide degraded after 30 mm. On the other hand, tropicamide proved to be exceptionally stable to oxidative stress and no degradation was observed with 30% H2O2 even alter heating at 70 degrees C for 90 min. The mobile phase consisted of acetonitrile and water phase 70:30v/v (water phase: 5 mM sodium heptan sulphonate and 1% triethylamine MA) with phi of the mobile phase adjusted to 3.5 with ortophosphoric acid. The flaw rate was 2 mL min(-1). In order to confirm the method adequacy for intended purpose, the proposed method was validated. Finally, the proper validation of developed SIM performed according to the official guidelines proved that the method accomplishes its intended purpose.en
dc.publisherTaylor & Francis Inc, Philadelphia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Liquid Chromatography & Related Technologies
dc.subjectbenzalkonium chlorideen
dc.subjecteye dropsen
dc.subjectforced degradation studiesen
dc.subjectliquid chromatographyen
dc.subjecttropicamideen
dc.subjectvalidationen
dc.titleInvestigation of tropicamide and benzalkonium chloride stability using liquid chromatographyen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаленовић, Aнђелија; Јововић, М.; Костић, Нада; Јанчић-Стојановић, Биљана;
dc.citation.volume35
dc.citation.issue1-4
dc.citation.spage231
dc.citation.epage239
dc.citation.other35(1-4): 231-239
dc.citation.rankM23
dc.identifier.wos000300967800018
dc.identifier.doi10.1080/10826076.2011.597072
dc.identifier.scopus2-s2.0-84856915415
dc.identifier.rcubconv_2600
dc.type.versionpublishedVersion


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