Assessment of beta-lactams retention in hydrophilic interaction chromatography applying Box-Behnken Design
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2012
Authors
Jovanović, MarkoRakić, Tijana
Jančić-Stojanović, Biljana

Malenović, Anđelija

Ivanović, Darko
Medenica, Mirjana
Article (Published version)

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In this paper, the retention prediction models for mixture of beta-lactam antibiotics analyzed by hydrophilic interaction chromatography (HILIC) are presented. The aim of the study was to investigate the retention behavior of some organic acids and amphoteric compounds including cephalosporins (cefotaxime, cefalexin, cefaclor, cefuroxime, and cefuroxime axetil) and penicillins (ampicillin and amoxicillin). Retention of substances with acidic functional group in HILIC is considered to be interesting since the majority of publications in literature are related to basic compounds. In the beginning of the study, classical silica columns were chosen for the retention analysis. Then, preliminary study was done and factors with the most significant influence on the retention factors were selected. These factors with the impact on the retention factors were investigated employing Box-Behnken design as a tool. On the basis of the obtained results the mathematical models were created and tested ...using ANOVA test and finally verified. This approach enables the presentation of chromatographic retention in many ways (three-dimensional (3-D) graphs and simple two-dimensional graphical presentations). All of these gave the possibility to predict the chromatographic retention under different conditions. Furthermore, regarding the structure of the analyzed compounds, the potential retention mechanisms in HILIC were suggested.
Keywords:
Box-Behnken design / Cephalosporins / Hydrophilic interaction chromatography / Penicillins / Retention prediction modelsSource:
Journal of Separation Science, 2012, 35, 12, 1424-1431Publisher:
- Wiley-VCH Verlag GMBH, Weinheim
Funding / projects:
- Modelling of different chromatographic systems with chemometrical approach in pharmaceutical analysis (RS-172052)
DOI: 10.1002/jssc.201200099
ISSN: 1615-9306
PubMed: 22740252
WoS: 000306010900004
Scopus: 2-s2.0-84863436523
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PharmacyTY - JOUR AU - Jovanović, Marko AU - Rakić, Tijana AU - Jančić-Stojanović, Biljana AU - Malenović, Anđelija AU - Ivanović, Darko AU - Medenica, Mirjana PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1714 AB - In this paper, the retention prediction models for mixture of beta-lactam antibiotics analyzed by hydrophilic interaction chromatography (HILIC) are presented. The aim of the study was to investigate the retention behavior of some organic acids and amphoteric compounds including cephalosporins (cefotaxime, cefalexin, cefaclor, cefuroxime, and cefuroxime axetil) and penicillins (ampicillin and amoxicillin). Retention of substances with acidic functional group in HILIC is considered to be interesting since the majority of publications in literature are related to basic compounds. In the beginning of the study, classical silica columns were chosen for the retention analysis. Then, preliminary study was done and factors with the most significant influence on the retention factors were selected. These factors with the impact on the retention factors were investigated employing Box-Behnken design as a tool. On the basis of the obtained results the mathematical models were created and tested using ANOVA test and finally verified. This approach enables the presentation of chromatographic retention in many ways (three-dimensional (3-D) graphs and simple two-dimensional graphical presentations). All of these gave the possibility to predict the chromatographic retention under different conditions. Furthermore, regarding the structure of the analyzed compounds, the potential retention mechanisms in HILIC were suggested. PB - Wiley-VCH Verlag GMBH, Weinheim T2 - Journal of Separation Science T1 - Assessment of beta-lactams retention in hydrophilic interaction chromatography applying Box-Behnken Design VL - 35 IS - 12 SP - 1424 EP - 1431 DO - 10.1002/jssc.201200099 ER -
@article{ author = "Jovanović, Marko and Rakić, Tijana and Jančić-Stojanović, Biljana and Malenović, Anđelija and Ivanović, Darko and Medenica, Mirjana", year = "2012", abstract = "In this paper, the retention prediction models for mixture of beta-lactam antibiotics analyzed by hydrophilic interaction chromatography (HILIC) are presented. The aim of the study was to investigate the retention behavior of some organic acids and amphoteric compounds including cephalosporins (cefotaxime, cefalexin, cefaclor, cefuroxime, and cefuroxime axetil) and penicillins (ampicillin and amoxicillin). Retention of substances with acidic functional group in HILIC is considered to be interesting since the majority of publications in literature are related to basic compounds. In the beginning of the study, classical silica columns were chosen for the retention analysis. Then, preliminary study was done and factors with the most significant influence on the retention factors were selected. These factors with the impact on the retention factors were investigated employing Box-Behnken design as a tool. On the basis of the obtained results the mathematical models were created and tested using ANOVA test and finally verified. This approach enables the presentation of chromatographic retention in many ways (three-dimensional (3-D) graphs and simple two-dimensional graphical presentations). All of these gave the possibility to predict the chromatographic retention under different conditions. Furthermore, regarding the structure of the analyzed compounds, the potential retention mechanisms in HILIC were suggested.", publisher = "Wiley-VCH Verlag GMBH, Weinheim", journal = "Journal of Separation Science", title = "Assessment of beta-lactams retention in hydrophilic interaction chromatography applying Box-Behnken Design", volume = "35", number = "12", pages = "1424-1431", doi = "10.1002/jssc.201200099" }
Jovanović, M., Rakić, T., Jančić-Stojanović, B., Malenović, A., Ivanović, D.,& Medenica, M.. (2012). Assessment of beta-lactams retention in hydrophilic interaction chromatography applying Box-Behnken Design. in Journal of Separation Science Wiley-VCH Verlag GMBH, Weinheim., 35(12), 1424-1431. https://doi.org/10.1002/jssc.201200099
Jovanović M, Rakić T, Jančić-Stojanović B, Malenović A, Ivanović D, Medenica M. Assessment of beta-lactams retention in hydrophilic interaction chromatography applying Box-Behnken Design. in Journal of Separation Science. 2012;35(12):1424-1431. doi:10.1002/jssc.201200099 .
Jovanović, Marko, Rakić, Tijana, Jančić-Stojanović, Biljana, Malenović, Anđelija, Ivanović, Darko, Medenica, Mirjana, "Assessment of beta-lactams retention in hydrophilic interaction chromatography applying Box-Behnken Design" in Journal of Separation Science, 35, no. 12 (2012):1424-1431, https://doi.org/10.1002/jssc.201200099 . .