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dc.creatorStevanović, Magdalena
dc.creatorSkapin, Sreco D.
dc.creatorBracko, Ines
dc.creatorMilenković, Marina
dc.creatorPetković, Jana
dc.creatorFilipić, Metka
dc.creatorUskoković, Dragan P.
dc.date.accessioned2019-09-02T11:29:45Z
dc.date.available2019-09-02T11:29:45Z
dc.date.issued2012
dc.identifier.issn0032-3861
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1733
dc.description.abstractSilver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (alpha, gamma, L-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV-VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsrestrictedAccess
dc.sourcePolymer
dc.subjectSilver nanoparticlesen
dc.subjectPLGAen
dc.subjectNanocompositesen
dc.titlePoly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potentialen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМиленковић, Марина; Ускоковић, Драган П.; Брацко, Инес; Скапин, Срецо Д.; Филипић, Метка; Петковић, Јана; Стевановић, Магдалена;
dc.citation.volume53
dc.citation.issue14
dc.citation.spage2818
dc.citation.epage2828
dc.citation.other53(14): 2818-2828
dc.citation.rankM21
dc.identifier.wos000305590000003
dc.identifier.doi10.1016/j.polymer.2012.04.057
dc.identifier.scopus2-s2.0-84862005815
dc.identifier.rcubconv_2654
dc.type.versionpublishedVersion


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